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DNA fingerprinting of tabanids (Diptera: Tabanidae) and their respective egg masses using PCR – restriction fragment profiling

Published online by Cambridge University Press:  02 April 2012

M. Iranpour ,
A.M. Schurko ,
G.R. Klassen  and
T.D. Galloway
M. Iranpour*
Affiliation:
Department of Entomology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
A.M. Schurko
Affiliation:
Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
G.R. Klassen
Affiliation:
Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
T.D. Galloway
Affiliation:
Department of Entomology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
*
1Corresponding author (e-mail: iranpour@ms.umanitoba.ca).
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Abstract

Polymerase chain reaction and subsequent restriction fragment profiling analysis were used to associate collected tabanid egg masses with their respective species of adult horse flies and deer flies (Diptera: Tabanidae) in Manitoba, Canada. The ribosomal DNA (rDNA) intergenic spacer between the 28S and 18S ribosomal RNA genes was used successfully to differentiate 34 species of adult tabanids representing five genera: Atylotus (1 sp.), Chrysops (10 spp.), Haematopota (1 sp.), Hybomitra (17 spp.), and Tabanus (5 spp.). rDNA was a suitable molecular target for identifying tabanid species because of the high level of interspecific variation when comparing fragment profiles among different species, and the corresponding minimal intraspecific variation among individuals of the same species. Restriction fragment profiles from 56 field-collected tabanid egg masses were compared with those previously obtained from adults of known species. Egg masses of five species were identified: Hybomitra lasiophthalma (Macquart), Hybomitra nitidifrons nuda (McDunnough), Chrysops aestuans van der Wulp, Chrysops excitans Walker, and Chrysops mitis Osten Sacken. We also provide physical descriptions of these tabanid egg masses along with pictures.

Résumé

La réaction en chaîne de la polymérase et l'analyse subséquente des profils des fragments de restriction nous ont servi à associer des masses d'oeufs de tabanidés récoltées en nature aux espèces correspondantes de taons et de mouches à chevreuil (Diptera: Tabanidae) au Manitoba, Canada. L'analyse de l'espace intergénétique ADNr des gènes 28S et 18S codant pour l'ARNr nous ont permis de distinguer avec succès 34 espèces des taons adultes appartenant à cinq genres (Atylotus (1 spp.), Chrysops (10 spp.), Haematopota (1 sp.), Hybomitra (17 spp.), et Tabanus (5 spp.)). L'ADNr est une bonne cible moléculaire pour l'identification des especès de taons parce qu'il y a un degré considérable de variation interspécifique entre les profils des fragments des différentes espèces et parce que la variation intraspécifique entre les indivus de la même espèce est minimale. Les profils des fragments de restriction des masses des oeufs de 56 espèces récoltées en nature ont ensuite été comparés avec ceux de masses d'oeufs de femelles identifiées à l'espèce. Les masses d'oeufs de cinq espèces ont été identifiées comme appartenant à Hybomitra lasiophthalma (Macquart), Hybomitra nitidifrons nuda (McDunnough), Chrysops aestuans van der Wulp, Chrysops excitans Walker et Chrysops mitis Osten Sacken. On trouvera la description et des photographies de ces masses d'oeufs.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 136 , Issue 5 , October 2004 , pp. 605 - 619
Copyright
Copyright © Entomological Society of Canada 2004

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