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HomeAdvances in Science and TechnologyAdvances in Science and Technology Vol. 102Effect of Dynamic Polyrotaxane Coating on...

Effect of Dynamic Polyrotaxane Coating on Cytoskeletal Signaling Expression of Adhering Stem Cells and Downstream Differentiations

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Abstract:

The effect of molecular mobility of the supramolecular surfaces on the intracellular signaling pathway and the downstream cell functions were investigated. As a supramolecular cell culture platform, polyrotaxanes (PRXs) with a varied number of threaded host molecules were synthesized and coated on glass surfaces. As a result, mesenchymal stem cells (MSCs) adhering on the dynamic PRX surface shows down-regulated RhoA-associated signaling pathway resulting in narrow and protruded adhering morphology. Moreover, mouse induced pluripotent stem (iPS) cells on the dynamic PRX surface shows highly up-regulated Rac1-associated signaling pathway resulting in enhanced N-cadherin expression and cardiomyogenic differentiations. Because MSCs and iPS cells on the polymer surfaces with low molecular mobility show the reverse tendency with those of the dynamic PRX surfaces, it could be concluded that the control of molecular mobility induced by supramolecular PRXs is effective to control cytoskeletal signaling pathway and the downstream stem cell differentiation into the preference cells or tissue.

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Periodical:

Advances in Science and Technology (Volume 102)

Pages:

37-42

DOI:

https://doi.org/10.4028/www.scientific.net/AST.102.37

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Online since:

October 2016

Authors:

Ji Hun Seo, Mitsuhi Hirata, Sachiro Kakinoki, Tetsuji Yamaoka, Nobuhiko Yui

Keywords:

Molecular Mobility, Polyrotaxane, Small GTPase Proteins, Stem Cell Commitment

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