<b>Agave syrup as a substrate for inulinase production by <i>Kluyveromyces marxianus</i> NRRL Y-7571

  • Luana Paula de Azevedo de Oliveira Universidade Federal do Recôncavo da Bahia
  • Tiago Felipe Oliveira Universidade Federal do Recôncavo da Bahia
  • Jonas Contiero Universidade Estadual Paulista "Julio de Mesquita Filho"
  • Márcia Luciana Cazetta Universidade Federal do Recôncavo da Bahia
Palavras-chave: yeast, microbial enzymes, agricultural substrate

Resumo

The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours.

 

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Publicado
2016-12-08
Como Citar
Oliveira, L. P. de A. de, Oliveira, T. F., Contiero, J., & Cazetta, M. L. (2016). <b>Agave syrup as a substrate for inulinase production by <i>Kluyveromyces marxianus</i&gt; NRRL Y-7571. Acta Scientiarum. Biological Sciences, 38(3), 283-289. https://doi.org/10.4025/actascibiolsci.v38i3.31489
Seção
Biotecnologia

 

0.6
2019CiteScore
 
 
31st percentile
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus