This study evaluated the effects of dehydration on the mechanical properties of adhesive resins and dentin, and on the microtensile bond strength (µTBS) of adhesives. Third molars were randomly bonded with Clearfil Mega Bond (MB) or Clearfil SE Bond 2 (SE). After water-storage (37°C; 24 h), µTBS was obtained in ‘wet’ (tested after 5 min of removal from storage) and ‘dehydrated’ (tested after 10, 15 min and 24 h) conditions by a universal tester (crosshead speed: 1 mm/min). Data were analyzed by two-way ANOVA and Duncan’s test. Hardness (H), Elastic modulus (E) and weight-loss of dentin beams and adhesive-resin discs were also monitored over time and analyzed by one-way repeated measures ANOVA and Bonferroni’s test (α=0.05). Significant differences in bond strength were observed for adhesives and for conditions. Except for dentin’s E, dehydration caused significant gradual changes in the H, E and weight of adhesive resins and dentin (p<0.05).