Abstract
In order to study the wide scope of structural information of biomolecules in the presence of biocompatible cold atmospheric pressure plasma jets (CAPJ), we used circular dichroism (CD) and fluorescence experiments. Biomolecules are very sensitive and are highly complex systems, exhibiting a substantial degree of structural variability in their folded state. In the present investigation, we compared the compatiblity of α-chymotrypsin (CT) in the presence of biocompatible CAPJ. Using circular dichroism (CD) and fluorescence experiments, we describe the associated structural changes in CT after a 5 min treatment with CAPJ. The modifications in the secondary structure of this β/β protein were quantified by using the CD spectra. A reasonable decrease was observed for β-strands after treatment with CAPJ as compared to buffer, which contributes to its deterioration power. The implications of our results from studies delineating the effects of CAPJ on enzyme solutions are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
M. Laroussi, I. Alexeff, J. P. Richardson and F. F. Dyer, IEEE Trans. Plasma Sci. 30, 158 (2002).
G. Cho, H. Lim, J. H. Kim, D. J. Jin, G. C. Kwon, E. H. Choi and H. S. Uhm, IEEE Trans. Plasma Sci. 39, 1234 (2011).
E. H. Choi, J. C. Ahn, M. W. Moon, J. G. Kim, M. C. Choi, C. G. Ryu, S. H. Choi, T. S. Cho, Y. Jung, G. S. Cho and H. S. Uhm, IEEE Trans. Plasma Sci. 39, 2160 (2011).
M. Moisan, J. Barbeau and S. Moreau, Int. J. Pharm. 226, 1 (2001).
H. X. Liu, J. R. Chen, L. Q. Yang and Y. Zhou, Appl. Surf. Sci. 254, 1815 (2008).
X. Zhang, J. Huang, X. Liu, L. Peng, L. Guo, G. Lv, W. Chen, K. Feng and S. Yang, J. Appl. Phys. 105, 063302 (2009).
J. F. Kolb, A-A. H. Mohamed, R. O. Price, R. J. Swanson, A. Bowman, R. L. Chiavarini, M. Stacey and K. H. Schonenbach, Appl. Phys. Lett. 92, 241501 (2008).
Y. C. Hong and H. S. Uhm, Appl. Phys. Lett. 89, 221504 (2006).
X. Zhang, M. Li, R. Zhou, K. Feng and S. Yang, Appl. Phys. Lett. 93, 021502 (2008).
X. T. Deng, J. J. Shi and M. G. Kong, J. Appl. Phys. 101, 074701 (2007).
Y. C. Hong, W. S. Kang, Y. B. Hong, W. J. Yi and H. S. Uhm, Phys. Plasmas 16, 123502 (2009).
Y. C. Hong, S. C. Cho and H. S. Uhm, Appl. Phys. Lett. 90, 141501 (2007).
P. Venkatesu, M. J. Lee and H. M. Lin, J. Phys. Chem. B 113, 5327 (2009).
P. Attri, P. Venkatesu and M. J. Lee, J. Phys. Chem. B 114, 1471 (2010).
P. Attri, P. Venkatesu, A. Kumar and N. Byrne, Phys. Chem. Chem. Phys. 13, 17023 (2011).
P. Attri, P. Venkatesu and A. Kumar, Phys. Chem. Chem. Phys. 13, 2788 (2011).
C. Branden and J. Tooze, Introduction to Protein Structure (Garland Publishing, Taylor & Francis Group, New York, 1999).
T. E. Creighton, Proteins, Structures and Molecular Properties (W. H. Freeman, New York, 1993).
I. Botos, E. Meyer, M. Nguyen, S. M. Swanson, J. M. Koomen, D. H. Russell and E. F. Meyer, J. Mol. Biol. 298, 895 (2000).
D. M. Blow, Acc. Chem. Res. 9, 145 (1976).
J. Kraut, Annu. Rev. Biochem. 46, 331 (1977).
T. A. Steitz and R. G. Shulman, Annu. Rev. Biophys. Bioeng. 11, 419 (1982).
H. Tsukada and D. W. Blow, J. Mol. Biol. 184, 703 (1985).
K. Takeda, A. Wada and Y. Moriyama, Colloid Polym. Sci. 268, 612 (1990).
V. Y. Levitsky, A. A. Panova and V. V. Mozhaev, Eur. J. Biochem. 219, 231 (1994).
T. de Diego, P. Lozano, S. Gmouh, M. Vaultier and J. L. Iborra, Biotechnol. Bioeng. 88, 916 (2004).
C. N. Pace and D. V. Laurents, Biochemistry 28, 2520 (1989).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Attri, P., Venkatesu, P., Kaushik, N. et al. Effects of atmospheric-pressure non-thermal plasma jets on enzyme solutions. Journal of the Korean Physical Society 60, 959–964 (2012). https://doi.org/10.3938/jkps.60.959
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.3938/jkps.60.959