QF-PCR in invasive prenatal diagnosis: a single-center experience in Turkey

Authors

ÖZGE ÖZER KAYA
ALTUĞ KOÇ
TAHA REŞİD ÖZDEMİR
ÖZGÜR KIRBIYIK
BERK ÖZYILMAZ
MEHMET ÖZEREN
DENİZ CAN ÖZTEKİN
CÜNEYT EFTAL TANER
YAŞAR BEKİR KUTBAY

DOI

10.3906/sag-1511-157

Abstract

Background/aim: QF-PCR has been used for more than 20 years. It is based on investigation of polymorphic short tandem repeats (STRs) and is widely used for prenatal rapid aneuploidy detection. Materials and methods: We report retrospectively our prenatal diagnosis results between January 2012 and May 2014 in Tepecik Training and Research Hospital Genetic Diagnostic Center. Prenatal diagnosis was recommended in 6800 high-risk pregnancies and 2883 patients agreed to invasive diagnosis. Chromosome analysis and QF-PCR were performed in all patients. Results: Normal results were reported in 2711 cases by fetal karyotyping and in 2706 cases by QF-PCR. Anomaly detection rates were similar for the two methods (5.09% for karyotyping and 4.02% for QF-PCR). Conclusion: QF-PCR is a fast and reliable prenatal diagnosis method in all indication groups and may be preferred as the sole prenatal investigation in patients without fetal ultrasonographic findings.

Keywords

Amniocentesis, chromosome aberrations, prenatal diagnosis, genetic counseling, polymorphism

First Page

142

Last Page

147

Recommended Citation

KAYA, ÖZGE ÖZER; KOÇ, ALTUĞ; ÖZDEMİR, TAHA REŞİD; KIRBIYIK, ÖZGÜR; ÖZYILMAZ, BERK; ÖZEREN, MEHMET; ÖZTEKİN, DENİZ CAN; TANER, CÜNEYT EFTAL; and KUTBAY, YAŞAR BEKİR (2017) "QF-PCR in invasive prenatal diagnosis: a single-center experience in Turkey," Turkish Journal of Medical Sciences: Vol. 47: No. 1, Article 21. https://doi.org/10.3906/sag-1511-157
Available at: https://journals.tubitak.gov.tr/medical/vol47/iss1/21