This paper describes experimental investigation on the three-dimensional internal flow structures of the acoustic levitated volatile droplets by stereo PIV. The acoustic levitation is one of the effective techniques to levitate and manipulate droplets in air. In order to apply the acoustic levitation to various fields such as the material science, analytical chemistry, drug discovery and so on, it is essential to understand the flow structures and transport phenomena on the acoustically levitated droplet. Our observation shows the internal flow structure is changed with the increase of the saturated vapor pressure of the levitated droplets. In the case of decane, nonane, octane, heptane, hexane and pentane droplets, the rotational motion is generated in each droplet. In the case of ethanol and methanol droplets, there is the flow from the equator toward the center of the droplet in the horizontal plane. In addition, the two or four vortices are generated in the horizontal plane with the increase of the droplet diameter.