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Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy
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Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Article DOI: 10.3791/64085-v • 08:13 min • September 16th, 2022
September 16th, 2022 •
1, 1, 2, 1, 1, 1, 1,2
1Department of Pathology and Translational Pathobiology, Louisiana State University Health Sciences Center-Shreveport, 2Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center-Shreveport

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Summary
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The present protocol describes a method of immuno-labeling a protein in the heart tissue sections using quantum dots. This technique provides a useful tool to visualize any protein's subcellular localization and expression at the ultrastructural level.

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Subcellular Localization Protein Heart Quantum Dots-mediated Immuno-labeling Transmission Electron Microscopy Ultrastructure Level Photobleaching Emission Spectra Electron Density Penetration In Tissues Fixation Etching Blocking Quantum Dots Concentration Grids Demonstration Anesthetizing Mice Chest Cavity Glutaraldehyde Sodium Cacodylate Buffer Fixatives
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