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Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells
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Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Article DOI: 10.3791/63062-v • 08:22 min • December 16th, 2021
December 16th, 2021 •
1, 1, 1, 1,2
1Division of Gastroenterology & Hepatology, Mayo Clinic, 2Division of Pediatric Gastroenterology, Mayo Clinic

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Summary
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Here we outline and demonstrate a protocol for primary mouse liver sinusoidal endothelial cell (LSEC) isolation. The protocol is based on liver collagenase perfusion, nonparenchymal cell purification by low-speed centrifugation, and CD146 magnetic bead selection. We also phenotype and characterize these isolated LSECs using flow cytometry and scanning electron microscopy.

Tags

Isolation Characterization Mouse Primary Liver Sinusoidal Endothelial Cells LSECs Protocol Collagenase Perfusion Ultracentrifugation Non-parenchymal Cells CD146 Magnetic Bead Selection Efficiency Applicability Purity Structure Function Functional Studies Molecular Studies Multi-omics Data Generation Intercellular Communication Culture Dishes Collagen Solution Recirculating Perfusion Apparatus Bleach Rinse Sterile Water Rinse Collagenase Solution Pump Rate
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