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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Article DOI: 10.3791/62395-v • 08:09 min • April 19th, 2021
April 19th, 2021 •
1, 1, 1,2
1Department of Chemistry, Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University

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Here, a detailed description of the protocol implemented in the laboratory for acquisition and analysis of 15N relaxation dispersion profiles by solution NMR spectroscopy is provided.

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15N CPMG Relaxation Dispersion Protein Conformational Dynamics μ-ms Timescale Cellular Processes Kinetics Thermodynamics Structural Aspects Nucleic Acids Biomolecules Ligand Nanoparticle Interactions NMR Experiment Pulse Program Folder Acquisition Software
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