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Journal / Biology / Inhibition of Wound Epidermis Formation via Full…
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Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration
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Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration

Article DOI: 10.3791/61522-v • 04:24 min • June 24th, 2020
June 24th, 2020 •
1,2
1Department of Stem Cell and Regenerative Biology, Harvard University, 2Department of Molecular and Cellular Biology, Harvard University

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Summary
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This article describes how to perform a surgical method to inhibit wound epidermis formation during axolotl limb regeneration by immediately suturing full thickness skin over the amputation plane. This method allows researchers to investigate the functional roles of the wound epidermis during the early stages of limb regeneration.

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Wound Epidermis Formation Full Skin Flap Surgery Axolotl Limb Regeneration Molecular Techniques Functional Techniques Efficient Protocol Technically Reproducible Wound Epidermis Function Surgical Method Limb Amputation Zeugopodial Skeletal Elements Dissecting Scissors Spring Scissors Small Incision Ventral Portion Of The Skin Forceps Midline Of The Zeugopodial Skeletal Elements Underlying Limb Tissues Duller Forceps Surgical Scissors Muscle Tissue Exposed Bone Full-thickness Skin Flap
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