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Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes
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Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes

Article DOI: 10.3791/61061-v • 08:37 min • August 27th, 2020
August 27th, 2020 •
1, 1, 1
1CNRS, Institut Jacques Monod, Université de Paris, F 75006, Paris, France

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We present here an improved protocol for the whole-mount preparation and immunostaining of Drosophila testes, suitable for confocal microscopy and also allowing reproducible and reliable labeling. We illustrate this protocol by 3D representation and quantification of colocalization experiments on the large S4-S5 spermatocytes.

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Immunostaining Whole-mount Drosophila Testes 3D Confocal Analysis Large Spermatocytes Antibody Penetration Fixation Protocol NP40 Heptane Tissue Shape Maintenance Deep Antibody Penetration Reproducible Labeling Quantifiable Acquisition Colocalization Studies Diploid Germ Cell Evaluation Anesthetize Flies Carbon Dioxide Exposure Fly Pad Dissecting Microscope Male Flies Decapitation Ringer's Buffer Silicone-coated Glass Slide Black Background Support Thorax And Abdomen Separation Testes Isolation Tissue Cleaning 4% Paraformaldehyde
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