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Journal / Cancer Research / Culture, Manipulation, and Orthotopic…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
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Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids

Article DOI: 10.3791/60469-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1
1Department of Life Sciences, Pohang University of Science and Technology
* These authors contributed equally

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Summary
Automatic Translation

This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.

Tags

Tumor Organoids Bladder Tumor In Vitro Cancer Model Culture Genetic Manipulation Orthotopic Transplantation Tumor Microenvironment Yubin Kim Graduate Student Murine Bladder Tumor HEPES DMEM Collagenase Type One And Two Thermolysin Centrifuge ACK Lysing Buffer Red Blood Cells
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