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JoVE Journal Neuroscience
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Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

Article DOI: 10.3791/60278-v • 05:52 min • October 20th, 2019
October 20th, 2019 •
1, 2,3, 1
1CERVO Brain Research Center, Department of Psychiatry and Neurosciences, Université Laval, 2Center for Neural Circuits and Behavior, Department of Neuroscience and Section of Neurobiology, Division of Biology, University of California at San Diego, 3Neurophotometrics Ltd.

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This protocol details how to implement and perform multi-fiber photometry recordings, how to correct for calcium-independent artifacts, and important considerations for dual-color photometry imaging.

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Multi-Fiber Photometry Neural Activity Freely-Moving Animals Fiber Photometry CSOM Imaging Genetically Encoded Custom Indicator Optical Fibers Specific Group Of Neurons Directing Or Responding To An Action Or Stimulus Accessible Way To Record Brain Regions Connection Or Genetic Profiles Signal To Noise Ekaterina Martianova Graduate Student Five Axis Translator Patch Cord Adaptor 470 Nanometer Excitation Light Auto Fluorescent Plastic Slide CMOS Camera Live Mode Gain Adjustment Lookup Table Focal Point Of The Objective X And Y Axes Adjustment Isotropic Circle
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