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Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Article DOI: 10.3791/59562-v • 08:05 min • October 7th, 2020
October 7th, 2020 •
1, 2,3, 2, 4, 4, 5, 1
1Department of Applied Chemistry, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 1Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, 2Faculty & Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 3Center for Ecological Research, Kyoto University, 4Department of Chemistry, Faculty of Science, Toho University, 5Graduate School of Integrated Arts and Sciences, Hiroshima University

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Summary
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A series of methods to determine the potential DNRA rate based on 14NH4+/15NH4+ analyses is provided in detail. NH4+ is converted into N2O via several steps and analyzed using quadrupole gas chromatography–mass spectrometry.

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Measurement Potential Rates Dissimilatory Nitrate Reduction Ammonium 14NH4+/15NH4+ Analyses Sequential Conversion N2O Coastal Ecosystems Marine Ecosystems Reservoirs Removing Nitrate Terrestrial Ecosystems Aquatic Environment Denitrification Anammox DNRA Protocol Environmental Samples N15 Labeled Ammonium N15 Labeled Nitrate Addition Advantage Nitrous Oxide Atmospheric Background Quadrupole Gas Chromatography Mass Spectrometer Isotope-ratio Mass Spectrometer PTFE Tape Aluminum Foil
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