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Journal / Developmental Biology / In Vitro Generation of Somite Derivatives from…
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Developmental Biology

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In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
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In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Article DOI: 10.3791/59359-v • 06:21 min • April 25th, 2019
April 25th, 2019 •
1, 2, 2
1Department of Life Science Frontiers, Center for iPS Cells Research and Application, Kyoto University, 2Department of Clinical Application, Center for iPS Cells Research and Application, Kyoto University

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Summary
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We present here a protocol for the differentiation of human induced pluripotent stem cells into each somite derivative (myotome, sclerotome, dermatome, and syndetome) in chemically defined conditions, which has applications in future disease modeling and cell-based therapies in orthopedic surgery.

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In Vitro Somite Derivatives Induced Pluripotent Stem Cells IPSC Regenerative Therapy Disease Research Myotome Dermatome Sclerotome Syndetome Cells Chemically Defined Conditions Musculoskeletal Disorder Drug Therapies Paraxial Mesoderm Embryogenesis Extracellular Matrix Solution Feeder-free Cell Culture Medium
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