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Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
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Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Article DOI: 10.3791/59087-v • 07:26 min • February 28th, 2019
February 28th, 2019 •
1, 1, 1, 1, 1,2, 3,4, 3,4, 1,5, 1
1Division of Biology and Chemistry - Laboratory for Biomolecular Research, Paul Scherrer Institut, 2Photon Science Division - SwissFEL, Paul Scherrer Institut, 3RIKEN SPring-8 Center, 4Department of Cell Biology, Graduate School of Medicine, Kyoto University, 5Department of Biology, ETH Zürich

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The success of a time-resolved serial femtosecond crystallography experiment is dependent on efficient sample delivery. Here, we describe protocols to optimize the extrusion of bacteriorhodopsin microcrystals from a high viscosity micro-extrusion injector. The methodology relies on sample homogenization with a novel three-way coupler and visualization with a high-speed camera.

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High Viscosity Extrusion Microcrystals Time-resolved Serial Femtosecond Crystallography X-ray Lasers Data Quality Adaptability High Speed Camera Extrusion Test Crystalladen Monoolein LCP MAG 7.9 Liquid Paraffin Vacuum Environment Syringe Syringe Coupler Lipid And Paraffin Mixing
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