A Subunit Vaccine Based on the VP2 Protein of Porcine Parvovirus 1 Induces a Strong Protective Effect in Pregnant Gilts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Antibodies
2.2. Construction of Recombinant Baculoviruses
2.3. Detection of VP2 Protein Expression and Purification
2.4. Transmission Electron Microscopy (TEM)
2.5. Analysis of Immunogenicity
2.6. HI Assay
2.7. Neutralization Test
2.8. Challenge Experiment
2.9. Statistical Analysis
3. Results
3.1. Expression of VP2 Protein
3.2. Electron Microscopy
3.3. Immunogenicity Study
3.4. Protection of Pregnant Gilts against PPV1 Challenge
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group | Sow No. | Vitality in Progeny | |||
---|---|---|---|---|---|
Dead | Mummified | Alive | Total | ||
ISA201VG 20 µg | A01 | 0 | 0 | 8 | 8 |
A02 | 0 | 0 | 12 | 12 | |
A03 | 0 | 0 | 13 | 13 | |
A04 | 0 | 0 | 9 | 9 | |
Commercial inactivated vaccine | B11 | 0 | 0 | 11 | 11 |
B12 | 0 | 0 | 9 | 9 | |
B13 | 0 | 0 | 10 | 10 | |
B14 | 0 | 0 | 12 | 12 | |
Control | C21 | 4 | 4 | 2 | 2 |
C22 | 0 | 12 | 0 | 0 | |
C23 | 4 | 6 | 1 | 1 | |
C24 | 0 | 9 | 0 | 0 |
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Ling, Z.; Zhang, H.; Chen, Y.; Sun, L.; Zhao, J. A Subunit Vaccine Based on the VP2 Protein of Porcine Parvovirus 1 Induces a Strong Protective Effect in Pregnant Gilts. Vaccines 2023, 11, 1692. https://doi.org/10.3390/vaccines11111692
Ling Z, Zhang H, Chen Y, Sun L, Zhao J. A Subunit Vaccine Based on the VP2 Protein of Porcine Parvovirus 1 Induces a Strong Protective Effect in Pregnant Gilts. Vaccines. 2023; 11(11):1692. https://doi.org/10.3390/vaccines11111692
Chicago/Turabian StyleLing, Zhanye, Huawei Zhang, Yingjin Chen, Leqiang Sun, and Junlong Zhao. 2023. "A Subunit Vaccine Based on the VP2 Protein of Porcine Parvovirus 1 Induces a Strong Protective Effect in Pregnant Gilts" Vaccines 11, no. 11: 1692. https://doi.org/10.3390/vaccines11111692