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Article

Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties

by
Gayathri Vijayakumar
1,
Hindhuja Kesavan
1,
Anisha Kannan
1,
Dhanalakshmi Arulanandam
1,
Jeong Hee Kim
2,
Kwang Jin Kim
2,
Hak Jin Song
3,
Hyung Joo Kim
3 and
Senthil Kumaran Rangarajulu
3,*
1
Department of Biotechnology, Rajalakshmi Engineering College, Chennai 602105, India
2
National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Korea
3
Department of Biological Engineering, Konkuk University, Seoul 143701, Korea
*
Author to whom correspondence should be addressed.
Processes 2021, 9(8), 1341; https://doi.org/10.3390/pr9081341
Submission received: 29 June 2021 / Revised: 27 July 2021 / Accepted: 27 July 2021 / Published: 30 July 2021
(This article belongs to the Special Issue Green Synthesis of Metallic Nanomaterials and Their Applications)
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Versions Notes

Abstract

:
To prevent microbial growth, chemical solvents are typically utilized. However, chemical solvents are hazardous to human health with low antimicrobial effects. Metallic-element (such as copper, silver, and gold) nanoparticles have many applications in biotechnology and biomedicine. Copper nanoparticles (CuNPs) are efficient owing to their antimicrobial, anti-inflammatory, and anti-proliferative properties. The objective of this study was to perform biogenic synthesis of copper nanoparticles using three different spices (star anise, seed of Illicium verum; nutmeg, seed of Myristica fragrans; and mace, membrane covering the seed of Myristica fragrans) and determine their antibacterial properties. CuNPs of spices were prepared by dissolving copper sulfate in the respective plant extract. They were then characterized by UV-Vis spectroscopy, FTIR, GC-MS, EDAX, and SEM analysis. Results of UV-Vis spectroscopy showed the maximum absorbance peak at 350 nm. SEM analysis showed that the sizes of these CuNPs were in the range of 150–200 nm. EDAX analysis confirmed the presence of copper and oxygen and revealed that copper existed in an oxidized form. FTIR spectroscopy showed the presence of different functional groups in these synthesized nanoparticles. GC-MS analysis revealed compounds such as Anethole D-limonene, heptadecanoic acid, 16-methyl-, methyl ester, myristene, methyl eugenol, and methyl stearate, indicating the presence of functional groups. The antibacterial activities of the three extracts from spices were analyzed using growth zone inhibition and TLC-bioautography methods. The results showed that star anise spice extract had the highest antibacterial activity. These results indicate that such CuNPs phyto-formulated with spice extracts having antibacterial properties could be used as potential therapeutics for microbial diseases.

1. Introduction

In modern years, there has been a continuing renewal of interest pertaining to the utilization of therapeutic and aromatic plants to produce antimicrobial drugs as plant-derived drugs are assured to be harmless and without side-effects [1]. People in different countries have a long-standing custom of employing a wide range of herbal products to heal diseases. Spices are indispensable components of Indian cuisines. They are also rich sources of dynamic antimicrobial compounds [2]. Nanoparticles have fascinated many researchers because they have unique characteristics such as size, shape, distribution, and morphology compared to bulk materials [3,4,5]. In all science fields, including the therapeutic field, metallic nanoparticles are being utilized. They are still alluring scientists to investigate new scopes due to their small sizes. These metal nanoparticles have advantages due to their small sizes, large surface areas, excellent chemical and optical properties, and good electrical conductivities [6]. Among several metallic-element nanoparticles, copper nanoparticles have been under an exceptional spotlight. Plant-based molecules are of great interest to the scientific community because they have a wide range of sizes and structures with various biological functions [7]. Copper nanoparticles are broadly used in batteries, optical devices, polymers, and multilayer metal ceramics. They are also used for drug delivery and as antimicrobials or antioxidants [8].
The potential for developing an antimicrobial into a medication appears gratifying from perspectives of medicine improvement and phytomedicine. Spices are known to possess various beneficial properties that are beneficial for human health due to their anti-oxidative, anti-inflammatory, anti-hepatotoxicity, anti-tumor, and anti-microbial effects. These biological activities of spices are due to the presence of various antimicrobial compounds. Inhibiting microbial growth and synthesizing antimicrobial compounds using spices have been topics of several studies [9]. Several spices such as oregano, clove, cinnamon, cumin, and thyme have been practiced for treating communicable diseases and protecting food as they have been theoretically demonstrated to have antimicrobial actions toward disease-causing organisms [10,11,12]. Energetic plant-based chemicals obtained from spices have a molecular basis for these activities. Furthermore, these antimicrobial activities are due to secondary metabolites of spices, the bulk of which are normally not dangerous materials for food or have side effects due to unfavorable properties [13].
Copper nanoparticles are tremendously minute. They typically have high surface-to-volume ratios. They play roles as antibacterial and antifungal agents. Their antimicrobial actions are brought by their near-proximity contact with microbial membranes and their metal ions released into solutions because nanoparticles undergo oxidization gradually in solutions with cupric ions liberated from them. They can generate lethal hydroxyl free radicals when the lipid membrane is adjacent to it. Free radicals can deconstruct lipids in cell membranes through oxidation and collapse membranes, leading to the leakage of the intracellular substances of cells through collapsed membranes. Such cells may not be able to maintain essential metabolic reactions, leading to modification within cells and necrobiosis [14].
Recently, green synthesis approaches have been utilized for the biosynthesis of nanoparticles using phyto-formulated bioactive products [3,15]. Biological compounds can act as strong reducers and stabilizers in the process of biogenic nanosynthesis, which is highly preferred to have an ecofriendly environment [16]. Spices are important plant condiments with many bioactive agents having various applications [11,12]. The objective of this study was to evaluate the efficacy of using extracts of three different spices (star anise, nutmeg, and mace) for the synthesis of CuNPs. Although several studies have been conducted on these spices, green-synthesized CuNPs using these spices and their antibacterial properties are still largely unexplored. Phyto-formulated copper nanoparticles obtained from these three spices were then characterized with various analytical methods. The antimicrobial activities of these extracts and synthesized nanoparticles were assayed with standard approaches.

2. Materials and Methods

2.1. Biosynthesis of Copper Nanoparticles

All chemical reagents used in this experiment were of analytical grade. The three spices (Illicium verum—star anise, Myristica fragrans—nutmeg, and mace (lacy coating of the nutmeg seed)) were collected from an herbal medicine shop located in Chennai, India. These three different spices were dried at 45 °C in an incubator. Dried spices were crushed to fine particles using a grinder in the dark at indoor temperatures (35 °C), placed into containers, and sealed. Typically, 5 g of each dried spice material was dissolved in 50 mL of water. The aqueous solution containing spice material was heated in a heating mantle at 60–80 °C for 20 min and filtered using Whatmann No.1 paper. After 0.5 M of copper sulfate solution was prepared freshly, it was then added to each spice extract at a 1:1 ratio and kept in the dark at 37 °C for 24 h. A color change of the solution from bluish green to brownish red was noted by visual examination. This confirmed the synthesis of CuNPs using spice extracts [17,18].

2.2. Analytical Characterization of Biogenic CuNPs

Biogenically synthesized CuNPs were initially analyzed by UV-Vis spectroscopy (U2900, Hitachi, Tokyo, Japan). The reduction of copper ions was evaluated using 1 mL of biogenically synthesized test samples along with a control (copper sulfate solution without plant extract). The absorbance was recorded with a resolution of 1 nm at wavelengths from 150 to 600 nm. A scanning electron microscopy (SEM) study was carried out to examine the size and surface morphology of the synthesized copper particle. For sample preparation, a thin gold layer was sputter-deposited onto the synthesized copper particle using an ion sputter coater with a gold target instrument. The thin gold layer produced was air-dried under ambient conditions. Images were then obtained using a Hitachi S-3400N instrument with an electron beam accelerated at 300 V to 30 kV. Signals were detected by secondary electron (SE)/backscattered electron (BSE) detectors with enlargement up to 300,000. Energy-dispersive X-ray (EDAX) spectroscopic analysis was performed to identify the existence of copper in synthesized particles. Elemental compositions for synthesized particles were determined in spot profile mode using an EDAX instrument coupled with SEM analysis. The existence of copper was determined using a detection graph. Elemental composition was estimated as a weight percentage. A dried pellet of synthesized copper nanoparticles was analyzed using a Perkin Elmer Fourier-Transform Infrared (FTIR) Spectroscopy C100599 Instrument with a resolution of 0.4 cm−1 at a working range of 350–7800 cm−1 [8]. The GC–MS technique was adopted for the detection and identification of phytochemical compounds present in copper nanoparticles of spices. It was carried out using a PerkinElmer GC Clarus 500 system (Waltham, MA, USA). The GC–MS detection was executed using electron impact mode with an ionization energy of 70 eV. The carrier gas was helium (99.999%) with a constant flow rate of 1 mL/min and the injection volume was 1 μL. Temperature settings were: injector temperature, 250 °C; ion-source temperature, 200 °C; and oven temperature, 110–280 °C [19].

2.3. Antibacterial Activity of Biogenic Copper Nanoparticles

Biogenically synthesized CuNPs at a concentration of 10 μg/mL were dispersed in sterile deionized water and used for an antimicrobial activity study using the well diffusion method [19]. Briefly, Mueller-Hinton agar plates were prepared and 100 µL of Staphylococcus aureus culture was spread evenly onto surfaces of these agar plates. Wells of comparable sizes (6 mm) were made on these plates and then loaded with as-synthesized CuNPs in triplicate. These plates were incubated at 37 °C for 24 h. The antimicrobial activity was determined based on the formation of the zone of inhibition (ZOI) in millimeters [20].

2.4. Antibacterial Activity of Spice Extract

One gram of granulate of plant material was immersed in 5 mL of hexane (1:5) for 24 h at 32 °C in an orbital shaker (120 rpm) incubator. The mixture was strained with Whatmann no. 1 paper. The filtrate was kept in petri dishes and the solvent was allowed to evaporate at 37 °C. For the evaluation of antimicrobial activity, samples were prepared by dissolving 100 mg of each extract in 1 mL of dimethyl sulfoxide (DMSO). The antimicrobial activity of each test extract was determined with the disc diffusion method [21]. For the antibacterial assay, the bacterial culture of S. aureus was inoculated into a nutrient agar broth medium and grown at 37 °C. Plates of agar media were prepared. Each plate was inoculated with an aliquot (100 µL) of bacterial suspension, which was spread evenly on the surface of the medium of the plate. After 15 min, discs were dipped in 2 μg/mL of test samples and placed on these plates. The positive control was prepared with gentamicin. All tests were carried out in triplicate. Plates were incubated at 37 °C for 24 h. The antimicrobial activity was assessed by measuring the diameter of ZOI in millimeters. The experiment was repeated three times for every extract against the test organism [21].

2.5. Extract Preparation and Chromatography

To prepare nanoparticles with spice extracts for chromatographic separation, 0.5 g of each nanoparticle sample was added to 1 mL of petroleum ether. Samples were separated on aluminum-backed thin-layer chromatography (TLC) plates using a mixture of chloroform/ethyl acetate/formic acid (5:4:1). Separated chemical compounds were detected using iodine vapor. After incubation at room temperature, compounds were observed with an ultraviolet-visible (UV-Vis) spectroscope [22].

2.6. TLC-Direct Bioautography

TLC-direct bioautography is suitable for the rapid chemical and biological testing of plant extracts. It is mainly based on the antimicrobial properties of evaluated substances. Briefly, developed plates were dried under a stream of fast-paced air and then incubated at 90 °C for 30 s. Bacterial cultures were prepared in a liquid medium. Prepared chromatograms were dipped in bacterial suspension. The TLC-chromatogram was then incubated overnight at 37 °C. For visualization of microbial growth, tetrazolium salts were employed. These salts could be converted to an intensely colored formazan by dehydrogenases of living microorganisms. These salts were sprayed onto the chromatogram and incubated at 37 °C for 3 to 4 h. The formation of clear white areas aligned with the purple backdrop on the TLC plate, indicating an antimicrobial effect of the test sample [23,24].

3. Results and Discussion

3.1. Nanoparticle Synthesis

The synthesis of copper nanoparticles was performed by adding copper sulfate solution (CuSO4·5H2O) to the extract at a 1:1 ratio. A color change of the aqueous mixture was observed after 24 h of incubation. Visual confirmation of the color change from the initial color to a brown color was recorded (Figure 1). An increase in the intensity of color with time indicated an enhanced production of CuNPs. In addition, the synthesis was confirmed by UV-Vis spectroscopy with a characteristic peak recorded at 250 nm without peaks recorded between 150 nm and 600 nm for the control (copper sulfate solution), as shown in Figure 2. This showed the reduction of Cu ions to CuNPs with a characteristic peak in the absorption range from 200 nm to 300 nm. In this study, all three test samples (star anise, nutmeg, and mace) showed the existence of a single plasma resonance peak at 250 nm, indicating the synthesis of biogenic CuNPs in the test solution, similar to CuNPs synthesized by using Caesalpinia bonducella seed extract via a green synthetic pathway [6], which also showed the maximum absorption peak at 250 nm.

3.2. SEM Analysis

The structures and shapes of biogenic CuNPs were revealed by scanning electron microscopy. The maximum size of these CuNPs was 270 nm. Their structures were found to have distinct shapes. The dimensions of these nanoparticles were found to be within the nanoscale range. Biogenic CuNPs prepared with extracts of star anise, nutmeg, and mace had sizes of 210–270 nm, 170–210 nm, and 150–220 nm, respectively (Figure 3). Thus, typical sizes of copper nanoparticles synthesized in this study ranged from 150 nm to 270 nm. Green-synthesized CuNPs with aloe barbadensis leaf extract have been reported to have sizes of 80 nm to 120 nm [25]. CuNPs prepared with clove bud extract have sizes of ~12 nm [26]. Cuprous oxide nanoparticles (Cu2O NPs) produced by the chemical reduction of copper sulfate salt in water-in-oil microemulsion solution using NaBH4 as a reductant have sizes of ~250 nm to ~550 nm [27] based on SEM analysis.

3.3. EDAX Analysis

For elemental identification and quantitative composition determination, EDAX analysis was performed. It confirmed the presence of copper (Cu) and oxygen (O) along with a meager impurity of sulfur in trace amounts (Figure 4). All of the three tested spices samples showed the existence of very similar profiles in its elemental composition. Weight compositions of copper (Cu), oxygen (O), and sulfur (S) in these synthesized nanoparticles were found to be 88.20%, 11.78%, and 0.02% by mass, respectively. The stoichiometric fraction of the Cu:O molar ratio was found to be 1.8851, which is very close to 2:1 (molar ratio), proving that the obtained copper nanoparticles were in the form of cuprous oxide (Cu2O), consistent with results of previous studies [27,28]. Recently, researchers have shown increasing interest in finding the mechanism involved in the synthesis of CuNPs with green plant sources. Biomolecules such as flavonoids, proteins, tannins, phenols, and terpenoids in plants have been reported to be good reducing and stabilizing agents for CuONPs synthesis [29]. The existence of phenols and terpenoids in green-synthesized CuNPs with spices was confirmed in the present study. Several studies have discovered that phytochemicals in plant extracts can initially form complexes with ion salts and then reduce ions to form nanoparticles [18]. Biomolecules in plant extracts can usually react with copper ions to cause reduction, which subsequently transforms into copper oxide nanoparticles (CuONPs) [30]. A similar type of reaction was also observed in the present study, where EDAX analysis proved the presence of CuONPs.

3.4. Fourier-Transform Infrared (FTIR) and Gas Chromatography–Mass Spectrometry (GC–MS) Analysis

3.4.1. FTIR Characterization of CuNPs

FTIR investigation depicted possible biomolecules in plant extracts accountable for the stabilization of CuNPs and to examine functional groups of phytochemicals on NPs as adsorbent materials.
As shown in Figure 5, FTIR spectra for green-synthesized nanoparticles with star anise revealed bands at 3004.89 cm−1 corresponding to =CH stretching, proving the presence of aromatic –H; 2923.88 cm−1 corresponding to OCH3 stretching; 2866.46 cm−1 corresponding to –CH stretching proving the presence of aromatic C–H; and 1745.4 cm−1, 1608.52 cm−1, and 1100.06 cm−1 corresponding to C=O stretching, C=C bending, and C–O stretching, respectively, proving the presence of an ester group, aryl C=C group, and aliphatic C–O group, respectively. The FTIR spectrum attributed to OCH3 stretching and –CH stretching has revealed the existence of a benzene ring in the obtained green-synthesized Cu nanoparticles of star anise [31]. The presence of these functional groups in green-synthesized nanoparticles with star anise indicates that it contains known structures such as anethole and methyl ester compounds such as stearate [32,33]. The existence of an ether group and aromatic cycle structure in star anise has been proven by earlier studies [34,35].
FT-IR spectra attained from green-synthesized nanoparticles of nutmeg (Figure 6) revealed bands at 3194.92 cm−1 corresponding to –OH stretching, proving the presence of carboxylic acid –OH; 2939.39 cm−1 corresponding to the C–H stretching of alkane C–H; 1704.54 cm−1 corresponding to C = O stretching of the ester group; 1430.48 cm−1 corresponding to C–H bending of alkane C–H; and 1264.88 cm−1 corresponding to C–O–C stretching of an ether group. The existence of these functional groups in green-synthesized nanoparticles of nutmeg depicted the presence of esters, alcohols, and phenolic compounds, similar to results of a previous study [36]. In one earlier study, the FTIR spectra on methanolic solvent extract and ethyl acetate extract of Myristica fragrans seeds also revealed the presence of a carbonyl group, alkane group, and ether group as functional groups of biological compounds such as alkaloids, steroids, tannins, flavonoids, phenolics, and glycosides, known to be secondary metabolites with antimicrobial properties [37]. It has been concluded that these functional groups are responsible for their antimicrobial properties [38].
FTIR spectra of green-synthesized nanoparticles of mace (Figure 7) disclosed bands at 3385.65 cm−1 corresponding to the O–H stretching, proving the presence of carboxylic acid –OH; 2922.84 cm−1 and 2852.79 cm−1 corresponding to C−H stretching; 1704.82 cm−1 corresponding to C = O stretching, confirming the presence of an ester group; 1632.3316 cm−1 and 1415.16 cm−1 both corresponding to aromatic C–C stretching; and 1024.28 cm−1 and 810.90 cm−1 corresponding to C–O–C stretching and the deformation vibration of C–H bonds in phenolic rings, respectively. These results of FTIR analysis of green-synthesized nanoparticles of mace indicated a unique set of biochemical markers such as aromatic rings, aldehydes, alkanes, alkenes, and phenols [39]. These were similar to previous studies that depicted the presence of ester, methyl ester, and phenolic compounds [40,41].

3.4.2. GC–MS Analysis

Gas chromatography–mass spectrometric (GC–MS) analysis showed sharp and broad peaks after 10 min of retention time. These obtained peaks of copper nanoparticles of spices were compared with the NIST library (ADMIS 2005). A few of them produced matching results (Table 1, Table 2 and Table 3).
About 57 compounds were identified by GC-MS analysis of Cu nanoparticles of star anise. Of these compounds, pentanoic acid, 2-ethylhexyl ester, D-limonene, α-pinene, anethole, chloroacetic acid, tetradecyl ester, trans-anethole, and methyl stearate were identified (Table 1) with reference to previous studies in the literature [12,42]. Most of these compounds had an alkane bond (hydrocarbons containing a single bond), a phenyl parent ring, and an alkene bond (containing one or more carbon–carbon double bonds). D-Limonene, α-pinene, anisole, chloroacetic acid, tetradecyl ester, and methyl stearate were also evident in the FTIR analysis of the present study. The same results have been revealed by other researchers [43]. Anethole and estragole compounds have been detailed by earlier studies [44,45]. Gholivand et al. [46] detected volatile constituents of star anise by GC-MS and disclosed that its key components are trans-anethole (81.4.0%), limonene (6.5%), chavicol (2.10%), and anisaldehyde (1.81%). The dried star anise fruit has nearly 8–12% essential oil, primarily anethole and fatty oil [47,48].
The GC-MS analysis of CuNPs of nutmeg revealed the presence of 78 compounds. Several compounds present in nutmeg were responsible for its biological activity and antimicrobial activity (Table 2). The major biological compounds in Cu nanoparticles of nutmeg based on the GC–MS analysis were 2-methoxy-4-(1-propenyl) phenol (RT: 10.458), octadecanoic acid (RT: 14.360), and cyclododecyne (RT: 26.458). These compounds have nutritional, pharmacological, and therapeutic importance [49,50,51]. FTIR analysis of the tested spices also showed the presence of esters, alcohols, and phenolic compounds. These organic compounds are responsible for the antimicrobial effect of green-synthesized nanoparticles [52,53]. In one earlier study, metal oxide nanoparticles synthesized with the aqueous seed extract of Myristica fragrans (nutmeg) were characterized by gas GC–MS. The results showed the existence of bioactive components that play an efficient role in reducing and capping mediators for transferring AgNO3 to AgNPs, thereby illuminating itself as an effective drug against multi-drug-resistant bacteria [54].
GC-MS analysis of copper nanoparticles of mace (Table 3) revealed many components similar to those of nutmeg. This is because mace is the fleshy red, net-like skin covering the kernel of nutmeg. These compounds included D-limonene (RT: 4.043), eugenyl methyl ether (RT: 10.964), myristic acid (RT: 11.913), myristic acid methyl ester (RT: 13.551), and methyl eugenol (RT: 16.41). Most of these analyzed compounds were found to be essential oils and phenolic compounds [55]. Key compounds such as D-limonene, methyl eugenol, eugenyl methyl ether, myristic acid, and myristic acid methyl ester were categorized as essential oils [56]. The presence of limonene [57], α-pinene, and myristene [58] in mace has been found by earlier analysis. CuONPs of spices exhibited catalytic activities in the 1,3-dipolar cycloaddition reaction between azides and terminal alkynes to form 1,2,3-triazoles. These triazole compounds have been reported to possess excellent antimicrobial activities [59,60]. They were proven to be responsible for the antimicrobial activity of synthesized nanoparticles. The antimicrobial action of essential oils is due to their solubility in the phospholipid bilayer of cell membranes [61].
Copper nanoparticles as a nano-encapsulation of essential oils show improved antimicrobial activities [62]. In the present study, the GC-MS analysis of green-synthesized copper nanoparticles of spices also revealed the presence of essential oils. An indispensable characteristic of essential oils is their hydrophobicity, which permits them to be separated into the lipid of the cell membrane of bacteria, thus affecting the membrane structure and making it leakier [63]. This can be the origin of the seepage of ions and other cellular molecules [64]. The seepage of numerous biomolecules into the cytoplasm such as proteins, amino acids, and carbohydrates is the major cause for the cell death of bacteria due to the inclusion of nanoparticles [65]. CuNPs can intermingle with the microbial cell wall because they are attracted by the carboxyl group present on the microbial exterior [66]. In the present study, the presence of carboxyl groups was proved by FTIR and GC-MS analysis. The production of reactive oxygen species (ROS), enzyme activity loss, membrane damage, protein dysfunction, and so on are responsible for the antimicrobial action of nanoparticles [67]. It has been discovered that when CuNPs come in contact with a bacterial cell, copper ions are released and engrossed on the cell wall, resulting in the generation of ROS and loss of membrane integrity [68].

3.5. Antibacterial Activity of Spice Extract by Disc Diffusion Method

Antibacterial activities (in terms of zone of inhibition) of spice extracts were assessed against S.aureus by the disc diffusion method (Figure 8). In the present study, extracts of star anise, nutmeg, and mace at a concentration of 100 mg/mL were tested. Results of the disc diffusion assay revealed that extracts of star anise, nutmeg, and mace had inhibitory effects on S. aureus. Inhibitory effects of extracts of Illcium verum, Myristica fragrans (nutmeg), and Myristica fragrans (mace) on S. aureus showed ZOIs of 1.03 ± 0.2 cm, 0.9 ± 0.2 cm, and 0.9 ± 0.1 cm, respectively (Table 4). The presence of essential oil in star anise [69], nutmeg, and mace [70] has already been reported. The solubility in water possessing essential oil components is precisely associated with the capacity to go through the cell walls of a bacterium [71]. It is additionally influenced by the cell wall of each kind of bacteria, with Gram-positive bacteria having thicker peptidoglycan layers than Gram-negative bacteria do [72]. The process of inhibiting a bacterial system includes damage to the cell membranes, inhibition of protein synthesis, and disruption of the biological functions through specific enzymes [73]. Terpenoids in essential oils of spices are characterized by their lability that can cause swelling of the cytoplasmic membrane to a greater extent, thereby exhibiting antimicrobial effects [61]. They can also disintegrate the cellular integrity and inhibit respiration and ion transportation activity [74]. Phenolic compounds in spice extracts might contribute to their antibacterial effects the most, whilst other constituents might contribute very little to such effects [75]. Compounds such as myristicin and macelignan [76] isolated from the plant seed exhibit good antibacterial activities against selected Gram-positive bacteria [77].

3.6. Antibacterial Activity of CuNPs by Well-Diffusion Method

Antimicrobial activities of spice extracts and synthesized Cu nanoparticles against S. aureus and ZOIs were determined using a well-diffusion process (Figure 9). ZOIs of CuNPs synthesized with Illicium verum (star anise), Myristica fragrans (nutmeg), and Myristica fragrans (mace) at concentrations of 10 µg/mL against S. aureus were 1.33 ± 0.089 cm, 1.06 ± 0.073 cm, and 1.26 ± 0.039 cm, respectively (Table 5). Gentamicin was employed as a positive control. It showed a ZOI of 2.12 ± 0.047 cm. These results revealed that biosynthesized CuNPs utilizing a spice extract displayed improved inhibition of bacteria than the extract itself [78]. A comparable result has been reported for CuNPs synthesized using Pinus merkusii plant extracts. ZOIs of CuNPs of pine flower extract against S. aureus were in the range between 0.608 cm and 1.221 cm [79]. Utilizing green-nanotechnology for the production of CuNPs has also been explored by Parikh and coworkers [80]. They revealed that the antibacterial activities of CuNPs against Escherichia coli, Bacillus megaterium, and Bacillus subtilis were higher than those of the extract. The investigators distinguished that this phytogenic method of nanoparticle synthesis was an inexpensive and eco-friendly technology. Hence, it might be valuable in the production of cost-effective nanomaterials, which will be potentially exploited for water purification, air-quality management, and antibacterial packaging [18]. The possible mechanism of the bactericidal impact of CuNPs is the delivery of Cu ions from nano copper and their entrance to bacterial cells with the consequent disruption of biochemical activities [81]. Bogdanovic et al. [82] suggested that bacterial cell wall action can stimulate the oxidation of CuNPs to release Cu++ ions, further reducing Cu++ ions to Cu+, and consequently leading to electrostatic attraction with plasma membrane-based reductases. Cu+ ions simply move through the lipid-bilayer into the cytosol and produce reactive oxygen species (ROS), leading to lipid peroxidation and oxidation of proteins. Another study has also stated that the antibacterial action of nanoparticles is due to the high-level conductivity of treated cells and discharge of cellular components [83].
Results indicated that CuNPs synthesized with star anise possessed higher antibacterial activities against the test organism (S. aureus) than CuNPs synthesized with nutmeg and mace. Similar results have been reported for the antimicrobial activity of CuNPs synthesized with Syzygium aromaticum (clove) bud extract against different bacterial species including Bacillus subtilis (ZOI = 4.2 cm) and Escherichia coli (ZOI = 3.3 cm) at a concentration of 200 µg/mL [26].

3.7. Thin-Layer Chromatography

The TLC separation of petroleum ether extracts of spices showed relevant spots. Chemical compounds of these spots were viewed under UV illumination. Spots were observed under short-wave. There was no spot under the long-wave of UV light [84]. Rf values and the colors of bands of petroleum ether extracts of spices were separated by TLC (Table 6). Rf values of star anise, nutmeg, and mace extracts were found to be 0.75, 0.41, and 0.47, respectively. These Rf values of nutmeg and mace indicate that an equivalent compound could be present in both spices as mace is the lacy coating of nutmeg seed. These spice extracts have been analyzed by previous studies. When spots are observed, similar Rf values have been reported [85], confirming their bioactive contents [86].

3.8. TLC–Direct Bioautography

TLC–bioautography is an accessible and easy means of analyzing plant extracts and pure elements for their impacts on infective microorganisms. It allows the straightforward detection of functional fractions. Antimicrobial activities of spice extracts were assessed by TLC–direct bioautography. The results showed the presence of white spots on TLC plates. These spots indicated the presence of antimicrobial activities of chosen spices (Figure 10).
The microbial growth was seen unswervingly on the surface of a TLC plate, eliminating spots of antimicrobials. Antibacterial activities of green-synthesized nanoparticles from spices were perceived by overlaying TLC plates with agar comprising S. aureus and then spraying with tetrazolium salt [87]. Oxidoreductases of living microorganisms can convert tetrazolium salt into a pinkish purple formazan [88,89]. The so-called inhibition zone was a creamy spot appearing against a purple background indicating the presence of antimicrobial agents. It was observed when green-synthesized nanoparticles were tested for antimicrobial activities. This method allowed for the finding of spots of growth inhibition of cultures immediately in the extract. The TLC plate was previously distributed with a broth culture comprising microorganisms [90]. In earlier studies, antifungal activities of nutmeg [91] and star anise [92] have been proven by TLC–bioautography.
Furthermore, after crossing the membrane, biogenic nanoparticles can work together with some important sites inside the cell, resulting in the antibacterial activity. It has been reported that CuNPs are liable for the disruption of metabolic pathways, formation of membrane pits, and oxidative stress development known to cause cell death [93], thereby showing antibacterial activity. In addition, metal NPs may put off bacterial replication and protein binding by countering with soft bases such as phosphorous and sulfur molecules of cellular deoxyribonucleic acid (DNA) [94]. Cu ions (Cu2+) can bind to DNA and engage in cross-linking of nucleic acid strands, leading to the incompetence in making helical structures. In a comparable way, these free CuNPs can attach to the cell membrane and enter into the bacterium via endocytosis [95].
Many plant-based CuO/CuNPs with established antibacterial effects tend to have antioxidant properties [96]. In an earlier study, Cissus vitiginea-mediated CuNPs have demonstrated antioxidant activities, which contribute to growth inhibition of urinary-tract-infection pathogens [97]. Likewise, CuO/CuNPs synthesized with spice extracts of star anise, nutmeg, and mace exhibited antibacterial activities in the present study possibly due to their antioxidant properties [98].

4. Conclusions

In this investigation, we instituted a phytoformulation technique for the green synthesis of CuNPs using extracts of spices (star anise, nutmeg, and mace), which showed strong antibacterial activities against S. aureus. These biogenic copper nanoparticles of 150–200 nm in size were further characterized with UV-Vis, SEM, and EDAX analysis. These copper nanoparticles showed stronger antibacterial activities through zone-of-inhibition studies. The results proved that these synthesized copper nanoparticles were more competent than spice extracts alone. The antimicrobial activities of nanoparticles were evaluated with TLC–bioautography. It also deep-rooted the presence of antimicrobial compounds in these extracts with comparatively similar Rf values, especially in copper nanoparticles of nutmeg and mace. This was again sturdily emphasized by the FTIR and GC-MS analysis of biosynthesized CuNPs of spices, revealing the presence of phenolic compounds, esters, alkanes, and other essential oils with the ability to damage the cell wall of a microorganism, thereby acting as powerful antimicrobial agents. The overall results of this study revealed that the green-biosynthesis of CuNPs from spices could be exploited for the development of novel herbal-formulated antibacterial agents with prospective applications in antimicrobial therapy.

Author Contributions

Conceptualization, reviewing and editing, S.K.R.; investigation and writing—original draft preparation, G.V.; methodology, H.K.; validation, A.K.; formal analysis, D.A.; data curation, J.H.K.; project administration, K.J.K.; resources, H.J.S.; supervision, H.J.K. All authors have read and agreed to the published version of the manuscript.

Funding

This study was carried out with the support of the ‘Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ016185022021)’, Rural Development Administration, Korea.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors have no conflict of interest relevant to this study to disclose.

Abbreviations

CuNPsCopper nanoparticles
CuOCopper oxide
NPsNanoparticles
SEMScanning electron microscopy
EDAXEnergy-dispersive X-ray analysis
FTIRFourier-transform infrared spectroscopy
GC-MSGas chromatography–mass spectrometry
TLCThin-layer chromatography
ZOIZone of inhibition
DMSODimethyl sulfoxide
UV-visUltraviolet-visible
ROSReactive oxygen species
RTRetention time
NISTNational Institute of Standards and Technology
SESecondary electron
BSEBackscattered electron
S. aureusStaphylococcus aureus

References

  1. Rai, M.; Acharya, D.; Wadegaonkar, P. Plant-Derived Antimycotic. In Current Trends and Future Prospects; Hartworth Press: New York, NY, USA, 2003; pp. 165–195. [Google Scholar]
  2. Dash, B.K.; Sultana, S.; Sultana, N. Antibacterial activities of methanol and acetone extracts of fenugreek (Trigonella foenum) and coriander (Coriandrum sativum). Life Sci. Med. Res. 2011, 27, 1–8. [Google Scholar]
  3. Balashanmugam, P.; Prabhu, D.; Devasena, T.; Hak, J.S.; Kim, K.W.; Jung, Y.S.; Song, H.J.; Kim, H.J.; Kumaran, R.S. Facile synthesis of silver nanoparticles using Asian spider flower and its in vitro cytotoxic activity against human breast carcinoma cells. Processes 2020, 8, 430. [Google Scholar]
  4. Shah, M.; Fawcett, D.; Sharma, S.; Tripathy, S.; Poinern, G. Green synthesis of metallic nanoparticles via biological entities. Materials 2015, 8, 7278–7308. [Google Scholar] [CrossRef] [Green Version]
  5. Chung, I.M.; Rahuman, A.A.; Marimuthu, S.; Kirthi, A.V.; Anbarasan, K.; Padmini, P.; Rajakumar, G. Green synthesis of copper nanoparticles using Eclipta prostrate leaves extract and their antioxidant and cytotoxic activities. Exp. Ther. Med. 2017, 14, 14–18. [Google Scholar]
  6. Saranya, S.; Agneeswaran, R.; Deepa, P.N. Green-synthesized rice-shaped copper oxide nanoparticles using Caesalpinia bonducella seed extract and their applications. ACS Omega 2020, 5, 1040–1051. [Google Scholar]
  7. Shakeel, A.; Mudasir, A.; Babu, L.S.; Saiqa, I. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. J. Adv. Res. 2016, 7, 17–28. [Google Scholar]
  8. Gayathri, V.; Nivedha, S.; Pujita, V.; Ivo, R.S. Green synthesis of copper nanoparticles using bracts of Musa paradisiaca (Monthan) and study of its antimicrobial and antioxidant activity. Res. J. Pharm. Technol. 2020, 13, 781–786. [Google Scholar] [CrossRef]
  9. Kim, J.H.; Campbell, B.C.; Jiujiang, Y.; Noreen, M.; Kathleen, L.C.; Russell, J.M.; Deepak, B.; Thomas, E.C. Examination of fungal stress response genes using Saccharomyces cerevisiae as a model system: Targeting genes affecting aflatoxin Biosynthesis by Aspergillus flavus Link. Appl. Microbiol. Biot. 2005, 67, 807–815. [Google Scholar] [CrossRef]
  10. Lai, P.K.; Roy, J. Antimicrobial and chemopreventive properties of herbs and spices. Curr. Med. Chem. 2004, 11, 1451–1460. [Google Scholar] [CrossRef]
  11. De, M.; De, A.K.; Banerjee, A.B. Antimicrobial screening of some Indian spices. Phytother. Res. 1999, 13, 616–618. [Google Scholar] [CrossRef]
  12. Arora, D.S.; Kaur, J. Antimicrobial activity of spices. Int. J. Antimicrob. Agents 1999, 12, I257–I262. [Google Scholar] [CrossRef]
  13. Cyrill, L.G.; Heran, Y.K.; Elena, V.B.; Andrey, A.Z.; Lariza, N.I. Plant secondary metabolites in the battle of drugs and drug-resistant bacteria: New heroes or worse clones of antibiotics? Antibiotics 2020, 9, 170. [Google Scholar]
  14. Khanna, P.K.; Gaikwad, S.; Adhyapak, P.V.; Singh, N.; Marimuthu, R. Synthesis and characterization of nanoparticles. Mater. Lett. 2007, 61, 4711–4714. [Google Scholar] [CrossRef]
  15. Pannerselvam, B.; Thiyagarajan, D.; Pazhani, A.; Thangavelu, K.P.; Kim, H.J.; Kumaran, R.S. Copperpod plant synthesized AgNPs enhance cytotoxic and apoptotic effect in cancer cell lines. Processes 2021, 9, 888. [Google Scholar] [CrossRef]
  16. Kathiravan, G.; Yamini, K.R.; Rajagopal, K.; Anandan, S.; Kim, K.W.; Jung, Y.S.; Song, H.J.; Kim, H.J.; Kumaran, R.S. Phytogenic synthesis of nano silver from Madagascar Periwinkle extracts and their angiogenic activities in Zebrafish embryos (ZFE). Nanosci. Nanotechnol. Lett. 2020, 12, 79–87. [Google Scholar] [CrossRef]
  17. Abd-Elkareem, J.I.; Bassuony, H.M.; Mohammed, S.M.F.; Heba, M. Eco-friendly methods of copper nanoparticles synthesis. J. Bionanosci. 2016, 10, 1–23. [Google Scholar] [CrossRef]
  18. Subhankari, I.; Nayak, P. Antimicrobial activity of Copper Nanoparticles synthesised by Ginger (Zingiber officinale) extract. World J. Nano Sci. Technol. 2013, 2, 10–13. [Google Scholar]
  19. Rizwana, S.; Umar, F.; Muhammad, R.S.; Sara, K.; Nadia, R.; Sadia, N.; Aliya, I.; Ajmal, K. Rapid synthesis of gold nanoparticles from Quercus incana and their antimicrobial potential against human pathogens. Appl. Sci. 2017, 7, 29. [Google Scholar]
  20. Thakur, S.; Tiwari, K.L.; Jadhav, S.K. Antibacterial screening of root extract of Asparagus racemosus. Curr. Trends Biotechnol. Pharm. 2015, 9, 147–150. [Google Scholar]
  21. Darshan, D.; Hitesh, J.; Tarun, K.; Manthan, K. Evaluation of antibacterial and antifungal activity of fenugreek (Trigonella foenum-graecum) extracts. Int. J. Pharm. Pharm. Sci. 2016, 8, 212–217. [Google Scholar]
  22. Shin-Ichi, I.; Yoshihiko, A. TLC bioautography guided detection and biological activity of antimicrobial compounds. Plant Pathol. J. 2015, 9, 16–26. [Google Scholar]
  23. Okusa, P.N.; Caroline, S.; Michel, D.; Pierre, D. Optimization of the Culture Medium Used for Direct TLC-Bioautography. Application to the Detection of Antimicrobial Compounds from Cordia gilletii De wild (Borgainaceae). J. Planar. Chromat. 2010, 23, 245–249. [Google Scholar] [CrossRef]
  24. Saikat, D.; Moumita, G.; Niloy, B.; Ritu, K.; Tarun, K.D. Bioautography and its scope in the field of natural product chemistry. J. Pharm. Anal. 2015, 5, 75–84. [Google Scholar]
  25. Madiha, B.; Zahid, Q.; Farwa, H.; Nida, M. Biosynthesis of Copper Nanoparticles by using Aloe barbadensis Leaf Extracts. Inter. Ped. Dent. Open Acc. J. 2018, 1, 000110. [Google Scholar]
  26. Wisam, J.A.; Haneen, A.J. A new paradigm shift to prepare copper nanoparticles using biological synthesis and evalution of antimicrobial activities. Plant Arch. 2018, 18, 2020–2024. [Google Scholar]
  27. Fisseha, A.B.; Shepherd, M.T.; Evans, M.N.C. Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents. Sci. Rep. 2020, 10, 16680. [Google Scholar]
  28. Kouti, M.; Matouri, L. Fabrication of nanosized cuprous oxide using fehling’s solution. Sci. Iran. 2010, 17, 73–78. [Google Scholar]
  29. Rezaie, A.B.; Montazer, M.; Rad, M.M. Photo and bioactivities along with UV protection properties on polyester fabric through green in-situ synthesis of cauliflower-like CuO nanoparticles. J. Photochem. Photobiol. B Biol. 2017, 176, 100–111. [Google Scholar] [CrossRef]
  30. Kerour, A.; Boudiadar, S.; Bourzami, R.; Allouche, B. Eco-friendly synthesis of cuprous oxide (Cu2O) nanoparticles and improvement of their solar photocatalytic activities. J. Soild State Chem. 2018, 263, 79–83. [Google Scholar] [CrossRef]
  31. Zhang, G.; Yuan, C.; Sun, Y. Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star anise Essential Oil. Molecules 2018, 23, 1126. [Google Scholar] [CrossRef] [Green Version]
  32. Haidi, C.; Tianmin, S. Study on the Different Method of Extraction of Star anise Oil. E3S Web Conf. 2020, 213, 03035. [Google Scholar]
  33. Jayanta, K.P.; Gitishree, D.; Sankhadip, B.; Sabyasachi, B.; Chethala, N.V.; Maria, P.R.; Han-Seung, S. Star anise (Illicium verum): Chemical compounds, antiviral properties, and clinical relevance. Phytother. Res. 2020, 34, 1248–1267. [Google Scholar]
  34. Fatima, S.S.; Bushra, A.M.A.A.; Zainab, S.A. Extraction and identification of oil extract from anise (Pimpinellaanisum L.) seeds and study of its antimicrobial activity. GJPACR 2015, 3, 1–6. [Google Scholar]
  35. Helin, L.; Xiaoyu, W.; Xin, L.; Xiaobing, C.; Yanjun, L.; Huaro, C.; Yongzhi, M. Multistage extraction of star anise and black pepper derivatives for antibacterial, antioxidant, and anticancer activity. Front. Chem. 2021, 9, 660138. [Google Scholar]
  36. Sharma, G.; Sharma, A.R.; Kurian, M.; Bhavesh, R.; Nam, J.S.; Lee, S.S. Green synthesis of silver nanoparticle using Myristica fragrans (nutmeg) seed extract and its biological activity. Dig. J. Nanomater. 2014, 9, 325–332. [Google Scholar]
  37. Vibala, B.V.; Praseetha, P.K. Evaluating the Biological potential of phyto-compounds from Myristica fragrans seeds. Indian J. Public Health Res. Dev. 2019, 10, 1260–1266. [Google Scholar] [CrossRef]
  38. Ajithkumar, K.G.; Sunil Kesavadeth, G.; Pratheepkumar, V.; Sureshkumar, K.A.; Dineshbabu, K.V. FTIR spectral analysis of the phytocostituents in the Mace of Myristica fragrans for the detection of possible adultration with the mace of Myristica malabarica and Myristica beddomi. Int. J. Curr. Res. 2016, 8, 40897–40903. [Google Scholar]
  39. Oliveira, R.N.; Mancini, M.C.; Oliveira, F.C.S.; Passos, T.M.; Quility, B.; Thiré, R.M.S.; Mcguiness, G.B. FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing. Rev. Mater. 2016, 21, 767–779. [Google Scholar] [CrossRef] [Green Version]
  40. Emeka, G.A.; Nene, O.U.; Lawrence, U.S.E. Mineral, amino acid and fatty acid evaluations of Myristica fragrans seeds extracts. Sci. Afr. 2020, 10, e00567. [Google Scholar]
  41. Reena, S.; Pramod, P. Phytochemical studies on Myristica fragrance essential oil. BFAIJ 2012, 4, 62–64. [Google Scholar]
  42. Vimalavady, A.; Kadavul, K. Phytocomponents identified on the various extracts of stem of Hugonia mystax L. (Linaceae). Eur. J. Exp. Biol. 2013, 3, 73–80. [Google Scholar]
  43. Wenbo, Z.; Yan, Z.; Xueyan, Y.; Erlin, S. Determination of volatile compounds of Illicium verum Hook. f. using simultaneous distillation-extraction and solid phase microextraction coupled with Gas Chromatography-Mass Spectrometry. Trop. J. Pharm. Res. 2015, 14, 1879–1884. [Google Scholar]
  44. Fadzilah, H.; Hannis, F.M.; Ibtisam, A.W. Identification of compounds from the Illicium extract. EAJ 2018, 14, 1–9. [Google Scholar]
  45. Wong, Y.C.; Lee, P.P.; Nurdiyana, W.W.A. Extraction and antioxidative activity of essential oil from Star anise (Illicium verum). Orient. J. Chem. 2014, 30, 1159. [Google Scholar] [CrossRef] [Green Version]
  46. Gholivand, M.B.; Rahimi, N.M.; Chalabi, H. Determination of essential oil components of Star anise (Illicium verum) using simultaneous hydrodistillation-static headspace liquid-phase microextraction-gas chromatography mass spectrometry. Anal. Lett. 2009, 42, 1382–1397. [Google Scholar] [CrossRef]
  47. Li, S.G.; Li, M.Y.; Huang, Y.Z.; Hua, R.M.; Lin, H.F.; He, Y.J.; Wei, L.L.; Liu, Z.Q. Fumigant activity of Illicium verum fruit extracts and their effects on the acetylcholinesterase and glutathione S-transferase activities in adult Sitophilus zeamais. J. Pest. Sci. 2013, 86, 677–683. [Google Scholar] [CrossRef]
  48. Mohamad, H.S.; Wenli, S.; Qi, C. Chinese star anise and anise, magic herbs in traditional Chinese medicine and modern pharmaceutical science. Asian J. Med. Biol. Res. 2019, 5, 162–179. [Google Scholar]
  49. Ojinnaka, M.C.; Ubbor, S.C.; Okudu, H.O.; Uga, U. Volatile compound analysis of the leaves and seeds of Piper guineense using gas chromatography-mass spectrometry (GC-MS). Afr. J. Food Sci. 2016, 10, 327–332. [Google Scholar] [CrossRef] [Green Version]
  50. Yu, F.R.; Lian, X.Z.; Guo, H.Y.; McGuire, P.M.; Li, R.D.; Wang, R.; Yu, F.H. Isolation and characterization of methyl esters and derivatives from Euphorbia kansui (Euphorbiaceae) and their inhibitory effects on the human SGC-7901 cells. J. Pharm. Pharm. Sci. 2005, 8, 528–535. [Google Scholar]
  51. Abubakar, M.N.; Majinda, R.R.T. GC-MS analysis and preliminary antimicrobial activity of Albizia adianthifolia (Schumach) and Pterocarpus angolensis (DC). Medicines 2016, 3, 3. [Google Scholar] [CrossRef] [Green Version]
  52. Wissal, D.; Sana, B.; Sabrine, J.; Nada, B.; Wissem, M. Essential oils’ chemical characterization and investigation of some biological activities: A critical review. Medicines 2016, 3, 25. [Google Scholar]
  53. Muhammad, I.S.; Mahmood, A.; Ayesha, R.; Zahoor, Q.S.; Muhammad, A.Q.; Sania, M.; Amir, A. Chemical composition of the essential oils of nutmeg and mace by GCFID/MS indigenous to pakistan and evaluation of their biological activities. Lat. Am. J. Pharm. 2016, 35, 2176–2184. [Google Scholar]
  54. Balakrishnan, S.; Sivaji, I.; Kandasamy, S.; Senbagam, D.; Nachimuthu, M.; Guruswami, G. Biosynthesis of silver nanoparticles using Myristica fragrans seed (nutmeg) extract and its antibacterial activity against multidrug-resistant (MDR) Salmonella enterica serovar Typhi isolates. Environ. Sci. Pollut. Res. 2017, 24, 14758–14769. [Google Scholar] [CrossRef]
  55. Lai-Hao, W. Pharma sci-study the photochemical of fragrance allergens of eugenol derivatives in commercial essential oils and containing clove drugs using gas chromatography and liquid chromatography mass spectrometry. Pharmacol. Rep. 2019, 2, 1–8. [Google Scholar]
  56. Wu, S.; Rajeshkumar, S.; Madasamy, M.; Mahendran, V. Green synthesis of copper nanoparticles using Cissus vitiginea and its antioxidant and antibacterial activity against urinary tract infection pathogens. Artif. Cells Nanomed. Biotechnol. 2020, 48, 1153–1158. [Google Scholar] [CrossRef] [PubMed]
  57. Inga, M.; Mindaugas, M.; Liudas, I.; Zenona, K.; Robertas, L.; Jurga, B. GC-MS analysis of the composition of the extracts and essential oil from Myristica fragrans seeds using magnesium aluminometasilicate as excipient. Molecules 2019, 24, 1062. [Google Scholar]
  58. Hery, M.A.; Prietta, K.K.P.; Nur, A.H.; Anita, N. The analysis of nutmeg mace essential oil compound using gc-ms and antibacterial activity test toward Ecschericia coli and Staphylococcus aureus. MF 2020, 16, 1–8. [Google Scholar]
  59. Drishya, S.; Namitha, T.R.; Samera, P.J.; Vimala, J.; Pauison, M. Synthesis of silver and copper oxide nanoparticles using Myristica fragrans fruit extract: Antimicrobial and catalytic applications. Sustain. Chem. Pharm. 2020, 16, 100255. [Google Scholar]
  60. Bakr, F.A.; Ehab, A.L.; Hanan, A.M.; Ghada, E.A.A. Design and synthesis of new 4-pyrazolin-3-yl-1,2,3-triazoles and 1,2,3-triazol-4-yl-pyrazolin-1-ylthiazoles as potential antimicrobial agents. Eur. J. Med. Chem. 2012, 52, 263–268. [Google Scholar]
  61. Cleber, J.S.; Luiz, C.A.B.; Antonio, J.D.; Ricardo, M.M.; Antônio, L.P.; Iara, D.; Nélio, J.A. Chemical composition and antibacterial activities from the essential oils of myrtaceae species planted in Brazil. Quím. Nova. 2010, 33, 00010019. [Google Scholar]
  62. Hosseini, S.F.; Zandi, M.; Rezaei, M.; Faramandghavi, F. Two step methods for encapsulation of oregano essential oil in chitosan nanoparticles: Preparation, characterization and in vitro release study. Carbohydr. Poly. 2013, 95, 50–56. [Google Scholar] [CrossRef]
  63. Sikkema, J.; De Bont, J.A.M.; Poolman, B. Interactions of cyclic hydrocarbons with biological membranes. J. Biol. Chem. 1994, 269, 8022–8028. [Google Scholar] [CrossRef]
  64. Ultee, A.; Bennink, M.H.J.; Moezelaar, R. The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Appl. Environ. Microbiol. 2002, 68, 1561–1568. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  65. Slavin, Y.N.; Asnis, J.; Häfeli, U.O.; Hracio, B. Metal nanoparticles: Understanding the mechanisms behind antibacterial activity. J. Nanobiotechnol. 2017, 15, 65. [Google Scholar] [CrossRef] [PubMed]
  66. Ingle, A.; Duran, N.; Rai, M. Bioactivity, mechanism of action and cytotoxicity of copper-based nanoparticles: A review. Appl. Microbiol. Biotechnol. 2014, 98, 1001–1009. [Google Scholar] [CrossRef]
  67. Swarnkar, R.K.; Pandey, J.K.; Soumya, K.K.; Dwivedi, P.; Sundaram, S.; Prasad, S.; Gopal, R. Enhanced antibacterial activity of copper/copper oxide nanowires prepared by pulsed laser ablation in water medium. Appl. Phys. A 2016, 122, 704. [Google Scholar] [CrossRef]
  68. Raffi, M.; Mehrwan, S.; Bhatti, T.M.; Akhter, J.I.; Hameed, A.; Yawar, W.; Ul, H.M.M. Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli. Ann. Microbiol. 2010, 60, 75–80. [Google Scholar] [CrossRef]
  69. Cai, Y.Y.; Jing, F.Z.; Tian, W. Star anise essential oil: Chemical compounds, antifungal and antioxidant activities: A review. J. Essent. Oil Res. 2021, 33, 1. [Google Scholar]
  70. Abourashed, E.A.; El-Alfy, A.T. Chemical diversity and pharmacological significance of the secondary metabolites of nutmeg (Myristica fragrans Houtt.). Phytochem. Rev. Proc. PSE 2016, 15, 1035–1056. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  71. Karl, K.; Alexander, P.; Bernard, I.; Hildegunde, W.; Norbert, W. Antibacterial and Antifungal Properties of Essential Oil Components. J. Essent. Oil Res. 2011, 1, 119–128. [Google Scholar]
  72. Sarifah, N.; Indira, L.P.; Dwi, P.S. Antibacterial Activity of Nutmeg Oil, 2nd International Conference on Sustainable Agriculture and Food Security: A Comprehensive Approach. KnE Life Sci. 2017, 563–569. [Google Scholar] [CrossRef] [Green Version]
  73. Pelczar, M.J.; Reid, R.D. Microbiology; Mc Graw Hill Book Co.: New York, NY, USA, 1972. [Google Scholar]
  74. Cox, S.D.; Mann, C.M.; Karkham, J.L.; Bell, H.C.; Gustafson, J.E.; Warmington, J.R.; Wyllie, S.G. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J. Appl. Microbiol. 2000, 88, 170. [Google Scholar] [CrossRef] [PubMed]
  75. Dean, S.G.; Ritchie, G. Antibacterial properties of plant essential oils. Int. J. Food Micobiol. 1987, 5, 165–180. [Google Scholar] [CrossRef]
  76. Chung, J.Y.; Choo, J.H.; Lee, M.H.; Hwang, J.K. Anticariogenic activity of macelignan isolated from Myristica fragrans (nutmeg) against Streptococcus mutans. Phytomedicine 2006, 13, 261–266. [Google Scholar] [CrossRef] [PubMed]
  77. Zaleha, S.; Nadia, N.S.; Nordiyana, M.F.S.Y.; Carrie-Anne, H.S.; Jalifah, L.J. Antibacterial activity of Myristica fragrans against oral pathogens. Evid. Based Complement Alternat. Med. 2012, 2012, 825362. [Google Scholar]
  78. Suci, A.; Dwika, P.P.; Masruri, M.; Akhmad, S.; Sutiman, B.S. Green synthesis and characterization of copper nanoparticles using Piper retrofractum Vahl extract as bioreductor and capping agent. Heliyon 2020, 6, e04636. [Google Scholar]
  79. Masruri, M.; Pangestin, D.N.; Ulfa, S.M.; Riyanto, S.; Srihardyastutie, A.; Rahman, M.F. A potent Staphylococcus aureus growth inhibitor of a dried flower extract of Pinus Merkusii Jungh & De vriese and copper nanoparticle. IOP Conf. Ser. Mater. Sci. Eng. 2018, 299, 012072. [Google Scholar]
  80. Parikh, P.; Zala, D.; Makwana, B.A. Biosynthesis of copper nanoparticles and their antimicrobial activity. OA Lib. 2014, 1, 1–15. [Google Scholar] [CrossRef]
  81. Mohan, R.; Shanmugharaj, A.M.; Sung, H.R. An efficient growth of silver and copper nanoprticles on multiwalled carbon nanotube with enhanced anti microbial activity. J. Biomed. Mater. Res. B Appl. Biomater. 2011, 96, 119–126. [Google Scholar] [CrossRef]
  82. Bogdanović, U.; Lazić, V.; Vodnik, V.; Budimir, M.; Marković, Z.; Dimitrijević, S. Copper nanoparticles with high antimicrobial activity. Mater. Lett. 2014, 128, 75–78. [Google Scholar] [CrossRef]
  83. Raja, S.; Ramesh, V.; Thivaharan, V. Green biosynthesis of silver nanoparticles using Calliandra haematocephala leaf extract, their antibacterial activity and hydrogen peroxide sensing capability. Arab. J. Chem. 2017, 10, 253–261. [Google Scholar] [CrossRef] [Green Version]
  84. Dhanya, K.; Sasikuar, B. Molecular marker based adulteration setection in traded food and agricultural comodities of plant origin with special reference to Spices. Curr. Trends Biotechnol. Pharm. 2010, 4, 454–489. [Google Scholar]
  85. Virgil, D.; Anamaria, H.; Claudia, C. Thin-layer chromatography in spices analysis. J. Liq. Chromatogr. Relat. Technol. 2018, 41, 283–300. [Google Scholar]
  86. Seema, Y.M.; Chetana, D.B.; Mayuri, S.B.; Jadhav, S.L.; Gaikwad, D.D. Pharmacognostic, phytochemical, physicochemical and TLC profile study Mace (Aril) of Myristica malabarica Lamk. (Myristicaceae). JPHYTO 2017, 6, 329–334. [Google Scholar]
  87. Veronica, G.C.; Scott, A.R. Bioautography and chemical characterization of antimicrobial compound(s) in commercial water-soluble annatto extracts. J. Agric. Food Chem. 2005, 53, 524–2529. [Google Scholar]
  88. Choma, I.; Grzelak, E.M. Bioautography detection in thin-layer chromatography. J. Chromatogr. A 2011, 1218, 2684–2691. [Google Scholar] [CrossRef] [PubMed]
  89. Marston, A. Thin-layer chromatography with biological detection in phytochemistry. J. Chromatogr. A 2011, 1218, 2676–2683. [Google Scholar] [CrossRef]
  90. Navarro, V.; Rojas, G.; Delgado, G.; Lozoya, X. Antimicrobial compounds detected in Bocconia arborea extracts by a direct bioautographic method. Arch. Med. Res. Summer. 1998, 29, 191–194. [Google Scholar]
  91. Jing, Z.; Jiang-sheng, Z.; Bin, Y.; Guang-Ping, L.; Shao-Ping, L. Free radical scavenging activity and characterization of sesquiterpenoids in four species of Curcuma using a TLC bioautography assay and GC-MS analysis. Molecules 2010, 15, 7547–7557. [Google Scholar]
  92. Karina, M.; Szymulanska, R.; Ming, Z.; Chun-Tao, C. Phytochemical study of Illicium angustisepalum and its biological activities. Acta Pharm. Sin. B 2017, 7, 485–490. [Google Scholar]
  93. Shende, S.; Ingle, A.P.; Gade, A.; Rai, M. Green synthesis of copper nanoparticles by Citrus medica Linn. (Idilimbu) juice and its antimicrobial activity. World J. Microbiol. Biotechnol. 2015, 3, 865–873. [Google Scholar] [CrossRef]
  94. Weria, W.; Saadi, S.; Jahanshir, A.; Somaieh, H.; Shima, Y.; Filippo, M. Enhancement of the antifungal activity of thyme and dill essential oils against Colletotrichum nymphaeae by nano-encapsulation with copper NPs. Ind. Crops Prod. 2019, 132, 213–225. [Google Scholar]
  95. Tamayo, L.; Azócar, M.; Kogan, M.; Riveros, A.; Páez, M. Copper-polymer nanocomposites: An excellent and cost-effective biocide for use on antibacterial surfaces. Mater. Sci. Eng. C 2016, 69, 1391–1409. [Google Scholar] [CrossRef]
  96. Zhao, H.W.; Su, H.T.; Ahmeda, A.; Sun, Y.Q.; Li, Z.Y.; Zangeneh, M.M.; Nowrozi, M.; Zangeneh, A.; Moradi, R. Biosynthesis of copper nanoparticles using Allium eriophyllum Boiss leaf aqueous extract; characterization and analysis of their antimicrobial and cutaneous wound-healing potentials. Appl. Organomet. Chem. 2020, e5587. [Google Scholar] [CrossRef]
  97. Minakshi, A.T.; Subhesh, S.J.; Khongdet, P.; Ravi, M.; Yaser, Q.; Haribabu, V.V. X-ray diffraction (XRD) analysis and evaluation of antioxidant activity of copper oxide nanoparticles synthesized from leaf extracts of Cissus vitiginea. Mater. Today Proc. 2021, in press. [Google Scholar] [CrossRef]
  98. Velsankar, K.; Kumar, R.M.A.; Preethi, R.; Muthulakshmi, V.; Sudhahar, S. Green synthesis of CuO nanoparticles via Allium sativum extract and its characterizations on antimicrobial, antioxidant, antilarvicidal activities. J. Environ. Chem. Eng. 2020, 8, 104123. [Google Scholar] [CrossRef]
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Figure 1. Phyto-formulated biosynthesis of CuNPs. (A) Plant materials of star anise (1), nutmeg (2), and mace (3) used for making extracts; (B) a mixture of copper sulfate and plant extract of star anise (1), nutmeg (2), or mace (3); (C) synthesis of CuNPs with star anise (1), nutmeg (2), or mace (3) and characteristic color change after 24 h of incubation; (D) copper sulfate aqueous solution (control).
Figure 1. Phyto-formulated biosynthesis of CuNPs. (A) Plant materials of star anise (1), nutmeg (2), and mace (3) used for making extracts; (B) a mixture of copper sulfate and plant extract of star anise (1), nutmeg (2), or mace (3); (C) synthesis of CuNPs with star anise (1), nutmeg (2), or mace (3) and characteristic color change after 24 h of incubation; (D) copper sulfate aqueous solution (control).
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Figure 2. UV-Vis spectroscopic analysis of test samples after 24 h of incubation, showing phytogenic synthesis of CuNPs with the maximum absorption at 250 nm.
Figure 2. UV-Vis spectroscopic analysis of test samples after 24 h of incubation, showing phytogenic synthesis of CuNPs with the maximum absorption at 250 nm.
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Figure 3. SEM images of biogenic CuNPs prepared with extracts of spices. (A) Nutmeg (150 nm to 220 nm), (B) star anise (210 nm to 270 nm), and (C) mace (150 nm to 220 nm).
Figure 3. SEM images of biogenic CuNPs prepared with extracts of spices. (A) Nutmeg (150 nm to 220 nm), (B) star anise (210 nm to 270 nm), and (C) mace (150 nm to 220 nm).
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Figure 4. EDAX analysis of green-synthesized CuNPs from star anise showing peaks representing the presence of copper (indicated by arrows).
Figure 4. EDAX analysis of green-synthesized CuNPs from star anise showing peaks representing the presence of copper (indicated by arrows).
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Figure 5. FT-IR spectra of green-synthesized CuNPs of star anise showing probable functional groups in synthesized nanoparticles.
Figure 5. FT-IR spectra of green-synthesized CuNPs of star anise showing probable functional groups in synthesized nanoparticles.
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Figure 6. FTIR spectra of biosynthesized CuNPs with nutmeg showing probable functional groups in synthesized nanoparticles.
Figure 6. FTIR spectra of biosynthesized CuNPs with nutmeg showing probable functional groups in synthesized nanoparticles.
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Figure 7. FTIR spectra of biosynthesized CuNPs of mace showing probable functional groups in the synthesized nanoparticles.
Figure 7. FTIR spectra of biosynthesized CuNPs of mace showing probable functional groups in the synthesized nanoparticles.
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Figure 8. Growth of S. aureus showing the zone of inhibition (circled in red) in the presence of CuNPs formulated with spice extract: (A) star anise, (B) nutmeg, (C) mace, and (D) gentamycin, a positive control, by disc diffusion method.
Figure 8. Growth of S. aureus showing the zone of inhibition (circled in red) in the presence of CuNPs formulated with spice extract: (A) star anise, (B) nutmeg, (C) mace, and (D) gentamycin, a positive control, by disc diffusion method.
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Figure 9. Growth of S. aureus showing zones of inhibition (circled in red) of CuNPs formulated with spice extract: (A) star anise, (B) nutmeg, (C) mace, and (D) gentamycin (positive control) with a well-diffusion method.
Figure 9. Growth of S. aureus showing zones of inhibition (circled in red) of CuNPs formulated with spice extract: (A) star anise, (B) nutmeg, (C) mace, and (D) gentamycin (positive control) with a well-diffusion method.
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Figure 10. Antimicrobial activities of phyto-formulated CuNPs by TLC–direct bioautography, showing the appearance of antibacterial spots in the presence and absence of DMSO.
Figure 10. Antimicrobial activities of phyto-formulated CuNPs by TLC–direct bioautography, showing the appearance of antibacterial spots in the presence and absence of DMSO.
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Table
Table 1. GC-MS analysis of CuNPs of star anise.
Table 1. GC-MS analysis of CuNPs of star anise.
S.No.Retention TimeCompound NameMolecular Formula
14.159Pentanoic acid, 2-ethylhexyl esterC13H26O2
24.043D-LimoneneC10H16
36.58α-pineneC10H16
46.854AnetholeC10H12O
512.403Chloroacetic acid, tetradecyl esterC16H31ClO2
621.02Trans-AnetholeC10H12O
726.166Methyl stearateC19H38O2
Table
Table 2. GC-MS analysis of Cu nanoparticles of nutmeg.
Table 2. GC-MS analysis of Cu nanoparticles of nutmeg.
S.No.Retention TimeCompound NameMolecular Formula
14.0672-DecenalC10H18O
25.2247-Decen-2-oneC10H18O
310.4582-Methoxy-4-(1-propenyl)phenolC10H12O2
411.913Myristic acidC14H28O2
513.551Myristic acid methyl esterC15H30O2
626.162Heptadecanoic acid, 16-methyl-, methyl esterC19H38O2
726.458CyclododecyneC12H2O
Table
Table 3. GC-MS analysis of Cu nanoparticles of mace.
Table 3. GC-MS analysis of Cu nanoparticles of mace.
S.No.Retention TimeCompound NameMolecular Formula
14.043D-LimoneneC10H16
25.264Butanedioic acid, diethyl esterC8H14O4
310.964Eugenyl methyl etherC11H14O2
411.913Myristic acidC14H28O2
513.551Myristic acid methyl esterC15H30O2
616.41Methyl eugenolC11H14O2
726.162Heptadecanoic acid, 16-methyl-, methyl esterC19H38O2
Table
Table 4. ZOI values of CuNPs synthesized with spices by disc diffusion method.
Table 4. ZOI values of CuNPs synthesized with spices by disc diffusion method.
Scheme 1.Bacterial StainZOI in cm
Star aniseS. aureus1.03 ± 0.2
NutmegS. aureus0.9 ± 0.2
MaceS. aureus0.9 ± 0.1
Gentamycin
(positive control)		S. aureus2.2 ± 0.06
Table
Table 5. ZOI values of CuNPs synthesized with extracts of spices using a well-diffusion method.
Table 5. ZOI values of CuNPs synthesized with extracts of spices using a well-diffusion method.
SpicesBacterial StainZOI in cm
Star aniseS. aureus1.33 ± 0.089
NutmegS. aureus1.06 ± 0.073
MaceS. aureus1.26 ± 0.039
Gentamycin
(positive control)		S. aureus2.12 ± 0.047
Table
Table 6. Rf values of nutmeg, star anise, and mace by TLC.
Table 6. Rf values of nutmeg, star anise, and mace by TLC.
SpicesRf Value
Nutmeg0.41
Star anise0.75
Mace0.47
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Vijayakumar, G.; Kesavan, H.; Kannan, A.; Arulanandam, D.; Kim, J.H.; Kim, K.J.; Song, H.J.; Kim, H.J.; Rangarajulu, S.K. Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties. Processes 2021, 9, 1341. https://doi.org/10.3390/pr9081341

AMA Style

Vijayakumar G, Kesavan H, Kannan A, Arulanandam D, Kim JH, Kim KJ, Song HJ, Kim HJ, Rangarajulu SK. Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties. Processes. 2021; 9(8):1341. https://doi.org/10.3390/pr9081341

Chicago/Turabian Style

Vijayakumar, Gayathri, Hindhuja Kesavan, Anisha Kannan, Dhanalakshmi Arulanandam, Jeong Hee Kim, Kwang Jin Kim, Hak Jin Song, Hyung Joo Kim, and Senthil Kumaran Rangarajulu. 2021. "Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties" Processes 9, no. 8: 1341. https://doi.org/10.3390/pr9081341

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