Silanization of Chitosan and Hydrogel Preparation for Skeletal Tissue Engineering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Si-HPMC and Si-Chito Synthesis
2.3. Hydrogel Preparation
2.4. Viscosity Measurements
2.5. Syringeability
2.6. Gel Point Measurements
2.7. Viscoelastic Moduli and Breaking Strength
2.8. Adhesion
2.9. Cell Investigations
2.9.1. Cell Viability in 2D
2.9.2. Cell Viability in 3D/Cell Adhesion
2.10. In Vivo Experiment
2.10.1. Implants Preparation
2.10.2. Subcutaneous Implantation
2.10.3. Histological Experiments
2.11. Statistics
3. Results
3.1. Chitosan Functionalization
3.2. Rheology
3.3. Viscoelastic Properties
3.4. Tissue Adhesion
3.5. Cell Investigations
3.6. In Vivo Experiment
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Relative Quantity Chitosan/ICPTS | 1/1 | 1/1.5 | ||||||||||
Reaction time (H) | 1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 5 | ||
Uncontroled pH | grafting efficiency | + | + | ++ | ++ | ++ | + | + | ++ | ++ | ++ | |
Solubility | - | - | + | + | + | - | - | + | + | + | ||
Hydrogel formation | alone | - | - | + (syneresis) | + (syneresis) | + (syneresis) | - | - | + (syneresis) | + (syneresis) | + (syneresis) | |
mixt with Si-HPMC | - | - | + | + | + | - | - | + | + | + (syneresis) | ||
Controled pH | grafting efficiency | - | - | - | - | - | - | - | - | - | - | |
Solubility | - | - | - | - | - | - | - | - | - | - | ||
Hydrogel formation | alone | - | - | - | - | - | - | - | - | - | - | |
mixt with Si-HPMC | - | - | - | - | - | - | - | - | - | - | ||
Relative Quantity Chitosan/ICPTS | 1/2 | 1/2.5 | ||||||||||
Reaction time (H) | 1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 5 | ||
Uncontroled pH | grafting efficiency | + | + | ++ | ++ | ++ | + | + | ++ | ++ | ++ | |
Solubility | - | - | + | + | + | - | - | + | + | + | ||
Hydrogel formation | alone | - | - | + (syneresis) | + (syneresis) | + (syneresis) | - | - | + (syneresis) | + (syneresis) | + (syneresis) | |
mixt with Si-HPMC | - | - | + | + (syneresis) | + (syneresis) | - | - | + (syneresis) | + (syneresis) | + (syneresis) | ||
Controled pH | grafting efficiency | - | - | - | - | - | - | - | - | - | - | |
Solubility | - | - | - | - | - | - | - | - | - | - | ||
Hydrogel formation | alone | - | - | - | - | - | - | - | - | - | - | |
mixt with Si-HPMC | - | - | - | - | - | - | - | - | - | - |
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Réthoré, G.; Boyer, C.; Kouadio, K.; Toure, A.; Lesoeur, J.; Halgand, B.; Jordana, F.; Guicheux, J.; Weiss, P. Silanization of Chitosan and Hydrogel Preparation for Skeletal Tissue Engineering. Polymers 2020, 12, 2823. https://doi.org/10.3390/polym12122823
Réthoré G, Boyer C, Kouadio K, Toure A, Lesoeur J, Halgand B, Jordana F, Guicheux J, Weiss P. Silanization of Chitosan and Hydrogel Preparation for Skeletal Tissue Engineering. Polymers. 2020; 12(12):2823. https://doi.org/10.3390/polym12122823
Chicago/Turabian StyleRéthoré, Gildas, Cécile Boyer, Kouakou Kouadio, Amadou Toure, Julie Lesoeur, Boris Halgand, Fabienne Jordana, Jérôme Guicheux, and Pierre Weiss. 2020. "Silanization of Chitosan and Hydrogel Preparation for Skeletal Tissue Engineering" Polymers 12, no. 12: 2823. https://doi.org/10.3390/polym12122823