Influence of Bagging on Fruit Quality, Incidence of Peel Browning Spots, and Lignin Content of ‘Huangguan’ Pears
Abstract
:1. Introduction
2. Results
2.1. Effects of Bagging on the Phenotype and Quality Development of ‘Huangguan’ Pears
2.2. Effects of Bagging on the Incidence of Peel Browning Spots and Structure of ‘Huangguan’ Pears
2.3. Effects of Bagging on the Incidence of Peel Browning Spots, Total Phenolic Contents and Activities of Related Enzymes in ‘Huangguan’ Pears
2.4. Effects of Bagging on the Expressions of Genes Related to the Incidence of Peel Browning Spots in ‘Huangguan’ Pears
3. Discussion
3.1. Changes in Fruit Development
3.2. Mechanism of Peel Browning Spots
4. Materials and Methods
4.1. Materials and Treatment
4.2. Measurement Indexes and Methods
4.2.1. Counting of Lenticels
4.2.2. Observation of Pericarp Structure
4.2.3. Measurement of Quality Indicators
4.2.4. Determination of L, a, b and IAD
4.2.5. Measurement of Lignin Content
4.2.6. Measurement of Total Phenolic Content
4.2.7. Determination of Enzymatic Activity
4.2.8. RNA Extraction and Analysis of Gene Expression
4.3. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Bag Type | Incidences of Peel Browning Spots (%) | |
---|---|---|
Weeks after Bagging (Weeks after Full Bloom) | ||
7 (13) | 9 (15) | |
No bagging | 0 ± 0.00 d | 0 ± 0.00 d |
Single-layer paper bag | 0 ± 0.00 d | 13.33 ± 0.06 b |
Two-layer paper bag | 0 ± 0.00 d | 10.00 ± 0.00 c |
Triple-layer paper bag | 0 ± 0.00 d | 66.67 ± 0.06 a |
Genes | GenBank Accession No. | Forward Primer | Reverse Primer |
---|---|---|---|
PbCAD8 | XM_009363237.2 | 5′-CCAAAAACTTAAAACTGTTCACCAA-3′ | 5′-ACTCGTCTGTGAGCTCATGC-3′ |
PbCAD9 | XM_009363233.2 | 5′-GGGCACCAACCGAAGTTAAA-3′ | 5′-TGTTTTGTACCTCCCGCTGC-3′ |
PbCCR3 | XM_009371295.2 | 5′-GGTAGGCACTTGTGTGTGGA-3′ | 5′-TGGCAAACTGGGAACCTTGT-3′ |
PbCCR4 | XM_018651024.1 | 5′-CAAGGCTTGCAAAAAGGCCA-3′ | 5′-ACTCCGGGTTCATAACGACG-3′ |
PbCCR5 | XM_009356071.2 | 5′-GCAGCAAATAATGGGCGCTT-3′ | 5′-CGACTTGGGATGTCGGACTG-3′ |
Pb4CL6 | XM_009358640.2 | 5′-GCTTTATCGGGAAGGCTTGTC-3′ | 5′-AATGGCAGCACAGCTGAATATC-3′ |
PbC4H1 | XM_009376113.2 | 5′-AACTTCGAGCTTCTGCCTCC-3′ | 5′-CCCCAAGCATCAATCTACGC-3′ |
PbActin2 | GU830959 | 5′-GGACATTCAACCCCTCGTCT-3′ | 5′-ATCCTTCTGACCCATACCAACC-3′ |
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Guan, Y.; Qin, X.; Wei, C.; Feng, Y.; Cheng, Y.; Zhang, Y.; Guan, J. Influence of Bagging on Fruit Quality, Incidence of Peel Browning Spots, and Lignin Content of ‘Huangguan’ Pears. Plants 2024, 13, 516. https://doi.org/10.3390/plants13040516
Guan Y, Qin X, Wei C, Feng Y, Cheng Y, Zhang Y, Guan J. Influence of Bagging on Fruit Quality, Incidence of Peel Browning Spots, and Lignin Content of ‘Huangguan’ Pears. Plants. 2024; 13(4):516. https://doi.org/10.3390/plants13040516
Chicago/Turabian StyleGuan, Yeqing, Xiaoli Qin, Chuangqi Wei, Yunxiao Feng, Yudou Cheng, Yang Zhang, and Junfeng Guan. 2024. "Influence of Bagging on Fruit Quality, Incidence of Peel Browning Spots, and Lignin Content of ‘Huangguan’ Pears" Plants 13, no. 4: 516. https://doi.org/10.3390/plants13040516