Effects of Drought Stress Induced by Hypertonic Polyethylene Glycol (PEG-6000) on Passiflora edulis Sims Physiological Properties
Abstract
:1. Introduction
2. Results
2.1. The Effects of Drought Stress Induced by Different Concentrations of Polyethylene Glycol (PEG) on the Fresh Weight of Passion Fruit Seedlings
2.2. The Effects of Drought Stress Induced by Different Concentrations of Polyethylene Glycol (PEG) on Physiological Properties of Passion Fruit Seedlings
2.2.1. Changes in Chlorophyll Content
2.2.2. Changes in Root Vitality
2.2.3. Changes in Soluble Protein and Proline Contents
2.2.4. Changes in Antioxidant Enzyme Activities
2.2.5. Changes in Malondialdehyde Content
3. Discussion
4. Materials and Methods
4.1. Experimental Material Preparation and Drought Stress
4.2. Experimental Reagents and Equipment
4.3. Determination of Morphological and Physiological Parameters
4.3.1. Determination of Growth Indices
4.3.2. Determination of Chlorophyll Content
4.3.3. Determination of Root Viability
4.3.4. Determination of Osmotic Modulating Substances
4.3.5. Antioxidant Enzyme Activity Assay and Determination of Malondialdehyde Content
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Orimoloye, I.R.; Belle, J.A.; Orimoloye, Y.M.; Olusola, A.O.; Ololade, O.O. Drought: A common environmental disaster. Atmosphere 2022, 13, 111. [Google Scholar] [CrossRef]
- García-Castro, A.; Volder, A.; Restrepo-Diaz, H.; Starman, T.W.; Lombardini, L. Evaluation of different drought stress regimens on growth, leaf gas exchange properties, and carboxylation activity in purple passionflower plants. J. Am. Soc. Hortic. Sci. 2017, 142, 57–64. [Google Scholar] [CrossRef] [Green Version]
- Xie, H.; Li, M.; Chen, Y.; Zhou, Q.; Liu, W.; Liang, G.; Jia, Z. Important physiological changes due to drought stress on oat. Front. Ecol. Evol. 2021, 9, 644726. [Google Scholar] [CrossRef]
- Xiong, S.; Wang, Y.; Chen, Y.; Gao, M.; Zhao, Y.; Wu, L. Effects of drought stress and rehydration on physiological and biochemical properties of four oak species in China. Plants 2022, 11, 679. [Google Scholar] [CrossRef] [PubMed]
- Akram, N.A.; Bashir, R.; Ashraf, G.; Bashir, S.; Ashraf, M.; Alyemeni, M.N.; Bajguz, A.; Ahmad, P. Exogenous α-Tocopherol Regulates the Growth and Metabolism of Eggplant (Solanum melongena L.) under Drought Stress. Plants 2023, 12, 237. [Google Scholar] [CrossRef] [PubMed]
- Ahanger, M.A.; Qi, M.; Huang, Z.; Xu, X.; Begum, N.; Qin, C.; Zhang, C.; Ahmad, N.; Mustafa, N.S.; Ashraf, M. Improving growth and photosynthetic performance of drought stressed tomato by application of nano-organic fertilizer involves up-regulation of nitrogen, antioxidant and osmolyte metabolism. Ecotoxicol. Environ. Saf. 2021, 216, 112195. [Google Scholar] [CrossRef]
- Ghani, M.I.; Saleem, S.; Rather, S.A.; Rehmani, M.S.; Alamri, S.; Rajput, V.D.; Kalaji, H.M.; Saleem, N.; Sial, T.A.; Liu, M. Foliar application of zinc oxide nanoparticles: An effective strategy to mitigate drought stress in cucumber seedling by modulating antioxidant defense system and osmolytes accumulation. Chemosphere 2022, 289, 133202. [Google Scholar] [CrossRef]
- Ghani, M.I.; Ali, A.; Atif, M.J.; Ali, M.; Amin, B.; Anees, M.; Cheng, Z. Soil amendment with raw garlic stalk: A novel strategy to stimulate growth and the antioxidative defense system in monocropped eggplant in the north of China. Agronomy 2019, 9, 89. [Google Scholar] [CrossRef] [Green Version]
- Ghani, M.I.; Ali, A.; Atif, M.J.; Ali, M.; Amin, B.; Cheng, Z. Arbuscular mycorrhizal fungi and dry raw garlic stalk amendment alleviate continuous monocropping growth and photosynthetic declines in eggplant by bolstering its antioxidant system and accumulation of osmolytes and secondary metabolites. Front. Plant Sci. 2022, 13, 849521. [Google Scholar] [CrossRef]
- Kandandapani, S.; Balaraman, A.K.; Ahamed, H.N. Extracts of passion fruit peel and seed of Passiflora edulis (Passifloraceae) attenuate oxidative stress in diabetic rats. Chin. J. Nat. Med. 2015, 13, 680–686. [Google Scholar] [CrossRef]
- Gioppato, H.A.; da Silva, M.B.; Carrara, S.; Palermo, B.R.Z.; Moraes, T.D.S.; Dornelas, M.C. Genomic and transcriptomic approaches to understand Passiflora physiology and to contribute to passionfruit breeding. Theor. Exp. Plant Physiol. 2019, 31, 173–181. [Google Scholar] [CrossRef]
- Dos Reis, L.C.R.; Facco, E.M.P.; Salvador, M.; Flôres, S.H.; de Oliveira Rios, A. Antioxidant potential and physicochemical characterization of yellow, purple and orange passion fruit. J. Food Sci. Technol. 2018, 55, 2679–2691. [Google Scholar] [CrossRef] [PubMed]
- Zeraik, M.L.; Pereira, C.A.M.; Zuin, V.G.; Yariwake, J.H. Maracujá: Um alimento funcional? Rev. Bras. Farmacogn. 2010, 20, 459–471. [Google Scholar] [CrossRef] [Green Version]
- Dhawan, K.; Dhawan, S.; Sharma, A. Passiflora: A review update. J. Ethnopharmacol. 2004, 94, 1–23. [Google Scholar] [CrossRef]
- Cerqueira-Silva, C.B.M.; Jesus, O.N.; Santos, E.S.; Corrêa, R.X.; Souza, A.P. Genetic breeding and diversity of the genus Passiflora: Progress and perspectives in molecular and genetic studies. Int. J. Mol. Sci. 2014, 15, 14122–14152. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Meletti, L.M.M. Avanços na cultura do maracujá no Brasil. Revista Brasileira Frutic. 2011, 33, 83–91. [Google Scholar] [CrossRef] [Green Version]
- da Silva Lima, L.K.; de Jesus, O.N.; Soares, T.L.; dos Santos, I.S.; de Oliveira, E.J.; Coelho Filho, M.A. Growth, physiological, anatomical and nutritional responses of two phenotypically distinct passion fruit species (Passiflora L.) and their hybrid under saline conditions. Sci. Hortic. 2020, 263, 109037. [Google Scholar] [CrossRef]
- Simon, P.; Karnatz, A. Effect of soil and air temperature on growth and flower formation of purple passionfruit (Passiflora edulis Sims var. edulis). Fruit Set Dev. XXI IHC 1982, 139, 83–90. [Google Scholar] [CrossRef]
- Menzel, C.M.; Simpson, D.R.; Dowling, A.J. Water relations in passionfruit: Effect of moisture stress on growth, flowering and nutrient uptake. Sci. Hortic. 1986, 29, 239–249. [Google Scholar] [CrossRef]
- Yan, C.; Song, S.; Wang, W.; Wang, C.; Li, H.; Wang, F.; Li, S.; Sun, X. Screening diverse soybean genotypes for drought tolerance by membership function value based on multiple traits and drought-tolerant coefficient of yield. BMC Plant Biol. 2020, 20, 321. [Google Scholar] [CrossRef]
- Kondo, T.; Morizono, H. Effects of Drought Stress on Flower Number in ‘Summer Queen’ Passion Fruit. Hortic. J. 2022, 91, 448–452. [Google Scholar] [CrossRef]
- Zhang, C.; Shi, S.; Liu, Z.; Yang, F.; Yin, G. Drought tolerance in alfalfa (Medicago sativa L.) varieties is associated with enhanced antioxidative protection and declined lipid peroxidation. J. Plant Physiol. 2019, 232, 226–240. [Google Scholar] [CrossRef] [PubMed]
- Almaghrabi, O.A. Impact of drought stress on germination and seedling growth parameters of some wheat cultivars. Life Sci. J. 2012, 9, 590–598. [Google Scholar]
- Murillo-Amador, B.; López-Aguilar, R.; Kaya, C.; Larrinaga-Mayoral, J.; Flores-Hernández, A. Comparative effects of NaCl and polyethylene glycol on germination, emergence and seedling growth of cowpea. J. Agron. Crop Sci. 2002, 188, 235–247. [Google Scholar] [CrossRef]
- Basu, S.; Roychoudhury, A.; Saha, P.P.; Sengupta, D.N. Comparative analysis of some biochemical responses of three indica rice varieties during polyethylene glycol-mediated water stress exhibits distinct varietal differences. Acta Physiol. Plant. 2010, 32, 551–563. [Google Scholar] [CrossRef]
- Pei, Z.; Ming, D.; Liu, D.; Wan, G.; Geng, X.; Gong, H.; Zhou, W. Silicon improves the tolerance to water-deficit stress induced by polyethylene glycol in wheat (Triticum aestivum L.) seedlings. J. Plant Growth Regul. 2010, 29, 106–115. [Google Scholar] [CrossRef]
- Sharif, P.; Seyedsalehi, M.; Paladino, O.; Van Damme, P.; Sillanpää, M.; Sharifi, A.A. Effect of drought and salinity stresses on morphological and physiological characteristics of canola. Int. J. Environ. Sci. Technol. 2018, 15, 1859–1866. [Google Scholar] [CrossRef]
- Li, W.; Wang, Y.; Zhang, Y.; Wang, R.; Xie, Z. Impacts of drought stress on the morphology, physiology, and sugar content of Lanzhou lily (Lilium davidii var. unicolor). Acta Physiol. Plant 2020, 42, 127. [Google Scholar] [CrossRef]
- Ayaz, M.; Ahmad, R.; Shahzad, M.; Khan, N.; Shah, M.M.; Khan, S.A. Drought stress stunt tomato plant growth and up-regulate expression of SlAREB, SlNCED3, and SlERF024 genes. Sci. Hortic. 2015, 195, 48–55. [Google Scholar] [CrossRef]
- Ahmed, M.; Kheir, A.M.; Mehmood, M.Z.; Ahmad, S.; Hasanuzzaman, M. Changes in Germination and Seedling Traits of Sesame under Simulated Drought. Phyton 2022, 91, 713–726. [Google Scholar] [CrossRef]
- Guo, Y.; Li, D.; Liu, L.; Sun, H.; Zhu, L.; Zhang, K.; Zhao, H.; Zhang, Y.; Li, A.; Bai, Z.; et al. Seed priming with melatonin promotes seed germination and seedling growth of Triticale hexaploide L. under PEG-6000 induced drought stress. Front. Plant Sci. 2022, 13, 932912. [Google Scholar] [CrossRef] [PubMed]
- Yang, X.; Lu, M.; Liu, H.; Zhang, Y.H.; Yin, H.; Wang, W.X.; Zhao, X.M.; Du, Y.G. Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress. Plant Physiol. Biochem. 2013, 62, 33–40. [Google Scholar] [CrossRef]
- Kayacetin, F. Assessment of safflower genotypes for individual and combined effects of drought and salinity stress at early seedling growth stages. Turk. J. Agric. For. 2022, 46, 601–612. [Google Scholar] [CrossRef]
- Si, C.; Li, Z.R.; Shen, N.D. Effects of PEG-6000 simulated drought stress and rehydration on the seed germination and sprout growth of Cicer arietinum L. J. Qinghai Univ. 2021, 39, 41–48. [Google Scholar] [CrossRef]
- Wang, Y.; Wang, Y.; Liu, Z.; Chen, S. Response mechanism of plants to drought stress. Horticulturae 2021, 7, 50. [Google Scholar] [CrossRef]
- Mafakheri, A.; Siosemardeh, A.F.; Bahramnejad, B.; Struik, P.C.; Sohrabi, Y. Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Aust. J. Crop Sci. 2010, 4, 580–585. [Google Scholar] [CrossRef]
- Wu, M.; Zhang, W.H.; Ma, C.; Zhou, J.Y. Changes in morphological, physiological, and biochemical responses to different levels of drought stress in Chinese cork oak (Quercus variabilis Bl.) seedlings. Russ. J. Plant Physiol. 2013, 60, 681–692. [Google Scholar] [CrossRef]
- Lobet, G.; Draye, X. Novel scanning procedure enabling the vectorization of entire rhizotron-grown root systems. Plant Methods 2013, 9, 1–11. [Google Scholar] [CrossRef] [Green Version]
- Gowda, V.R.; Henry, A.; Yamauchi, A.; Shashidhar, H.E.; Serraj, R. Root biology and genetic improvement for drought avoidance in rice. Field Crops Res. 2011, 122, 1–13. [Google Scholar] [CrossRef]
- Zhang, K.; Khan, Z.; Wu, H.; Khan, M.N.; Hu, L. Gibberellic Acid Priming Improved Rapeseed Drought Tolerance by Modulating Root Morphology, ROS Homeostasis, and Chloroplast Autophagy. J. Plant Growth Regul. 2022, 1–14. [Google Scholar] [CrossRef]
- Dinneny, J.R. Developmental responses to water and salinity in root systems. Annu. Rev. Cell Dev. Biol. 2019, 35, 239–257. [Google Scholar] [CrossRef] [PubMed]
- Zhang, X.; Yang, Z.; Li, Z.; Zhang, F.; Hao, L. Effects of drought stress on physiology and antioxidative activity in two varieties of Cynanchum thesioides. Braz. J. Bot. 2022, 43, 1–10. [Google Scholar] [CrossRef]
- Zhou, J.; Yuan, W.; Di, B.; Zhang, G.; Zhu, J.; Zhou, P.; Ding, T.R.; Qian, J. Relationship among Electrical Signals, Chlorophyll Fluorescence, and Root Vitality of Strawberry Seedlings under Drought Stress. Agronomy 2022, 12, 1428. [Google Scholar] [CrossRef]
- Altaf, M.A.; Shahid, R.; Ren, M.-X.; Naz, S.; Altaf, M.M.; Khan, L.U.; Tiwari, R.K.; Lal, M.K.; Shahid, M.A.; Kumar, R. Melatonin improves drought stress tolerance of tomato by modulating plant growth, root architecture, photosynthesis, and antioxidant defense system. Antioxidants 2022, 11, 309. [Google Scholar] [CrossRef]
- Cruz de Carvalho, M.H. Drought stress and reactive oxygen species: Production, scavenging and signaling. Plant Signal. Behav. 2008, 3, 156–165. [Google Scholar] [CrossRef] [Green Version]
- Cheng, L.; Han, M.; Yang, L.M.; Li, Y.; Sun, Z.; Zhang, T. Changes in the physiological characteristics and baicalin biosynthesis metabolism of Scutellaria baicalensis Georgi under drought stress. Ind. Crops Prod. 2018, 122, 473–482. [Google Scholar] [CrossRef]
- Zhao, T.; Pan, X.J.; Ou, Z.G.; Li, Q.; Zhang, W.E. Comprehensive evaluation of waterlogging tolerance of eleven Canna cultivars at flowering stage. Sci. Hortic. 2022, 296, 110890. [Google Scholar] [CrossRef]
- Zhong, L.; Liao, P.R.; Liu, C.Z.; Qian, J.P.; He, W.C.; Luo, B.; Yang, Q. Effects of drought stress on physiological and biochemical and chemical components of Cinnamomum cassia seedlings. China J. Chin. Mater. Med. 2021, 46, 2158–2166. [Google Scholar] [CrossRef]
- Guo, Y.Y.; Yu, H.Y.; Yang, M.M.; Kong, D.S.; Zhang, Y.J. Effect of drought stress on lipid peroxidation, osmotic adjustment and antioxidant enzyme activity of leaves and roots of Lycium ruthenicum Murr. Seedling. Russ. J. Plant Physiol. 2018, 65, 244–250. [Google Scholar] [CrossRef]
- Wang, Y.S.; Ni, F.; Yin, D.H.; Chen, L.J.; Li, Y.H.; He, L.X.; Zhang, Y.L. Physiological response of Lagerstroemia indica (L.) Pers. seedlings to drought and rewatering. Trop. Plant Biol. 2021, 14, 360–370. [Google Scholar] [CrossRef]
- Liu, T.; Zhu, S.; Fu, L.; Yu, Y.; Tang, Q.; Tang, S. Morphological and physiological changes of ramie (Boehmeria nivea L. Gaud) in response to drought stress and GA3 treatment. Russ. J. Plant Physiol. 2013, 60, 749–755. [Google Scholar] [CrossRef]
- Xiao, S.; Liu, L.T.; Wang, H.; Li, D.X.; Bai, Z.Y.; Zhang, Y.J.; Sun, H.C.; Zhang, K.; Li, C.D. Exogenous melatonin accelerates seed germination in cotton (Gossypium hirsutum L.). PLoS ONE 2019, 14, e0216575. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Kumari, N.; Yadav, M.; Sharma, V. Differential response of Brassica juncea cultivars to Al; consequences for chlorophyll a fluorescence, antioxidants and psb A gene. J. Plant Interact. 2018, 13, 496–505. [Google Scholar] [CrossRef] [Green Version]
- Cai, C.Y.; He, S.S.; An, Y.Y.; Wang, L.J. Exogenous 5-aminolevulinic acid improves strawberry tolerance to osmotic stress and its possible mechanisms. Physiol. Plant. 2020, 168, 948–962. [Google Scholar] [CrossRef] [Green Version]
- Lin, P.H.; Chao, Y.Y. Different Drought-Tolerant Mechanisms in Quinoa (Chenopodium quinoa Willd.) and Djulis (Chenopodium formosanum Koidz.) Based on Physiological Analysis. Plants 2021, 10, 2279. [Google Scholar] [CrossRef]
- Türkan, I.; Bor, M.; Özdemir, F.; Koca, H. Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci. 2005, 168, 223–231. [Google Scholar] [CrossRef]
- Sun, Y.D.; Du, X.H.; Zhang, W.J.; Sun, L.; Li, R. Seed germination and physiological characteristics of Amaranthus mangostanus L. under drought stress Advanced Materials Research. In Advanced Materials Research; Trans Tech Publications Ltd.: Wollerau, Switzerland, 2011; Volume 183–185, pp. 1071–1074. [Google Scholar] [CrossRef]
- Zgalla, H.; Steppe, K.; Lemeur, R. Photosynthetic, Physiological and Biochemical Responses of Tomato Plants to Polyethylene Glycol-Iinduced Water Deficit. J. Integr. Plant Biol. 2005, 47, 1470–1478. [Google Scholar] [CrossRef]
- Li, M.; Wang, H.; Zhao, X.; Lu, Z.; Sun, X.; Ding, G. Role of Suillus placidus in improving the drought tolerance of Masson Pine (Pinus massoniana Lamb.) seedlings. Forests 2021, 12, 332. [Google Scholar] [CrossRef]
- Lichtenthaler, H.K. Chlorophylls and Carotenoids, Pigments of Photosynthetic Biomembranes. Methods Enzymol. 1987, 148, 350–382. [Google Scholar] [CrossRef]
- Clemensson-Lindell, A. Triphenyltetrazolium chloride as an indicator of fine-root vitality and environmental stress in coniferous forest stands: Applications and limitations. Plant Soil 1994, 159, 297–300. [Google Scholar] [CrossRef]
- Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248–254. [Google Scholar] [CrossRef] [PubMed]
- Subramanyam, K.; Du Laing, G.; Van Damme, E.J.M. Sodium selenate treatment using a combination of seed priming and foliar spray alleviates salinity stress in rice. Front. Plant Sci. 2019, 10, 116. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Fridovich, I. Superoxide dismutases. Annu. Rev. Biochem. 1975, 44, 147–159. [Google Scholar] [CrossRef] [PubMed]
- Aebi, H. Catalase Methods of enzymatic analysis. Acad. Press 1974, 2, 673–684. [Google Scholar] [CrossRef]
- Heath, R.L.; Packer, L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 1968, 125, 189–198. [Google Scholar] [CrossRef]
Treatments | Fresh Weight (g) | ||||
---|---|---|---|---|---|
3 d | 6 d | 9 d | Rehydration | ||
Root | Control | 1.24 ± 0.12 a | 1.56 ± 0.20 a | 1.75 ± 0.13 a | 2.02 ± 0.12 a |
5% PEG-6000 | 1.20 ± 0.14 a | 1.51 ± 0.13 a | 1.60 ± 0.11 ab | 1.87 ± 0.14 a | |
10% PEG-6000 | 1.04 ± 0.10 ab | 1.26 ± 0.13 ab | 1.35 ± 0.20 bc | 1.44 ± 0.12 b | |
15% PEG-6000 | 0.88 ± 0.09 b | 1.02 ± 0.29 b | 1.09 ± 0.25 c | 1.32 ± 0.10 b | |
20% PEG-6000 | 0.85 ± 0.10 b | 0.97 ± 0.13 b | 1.02 ± 0.30 c | 1.23 ± 0.12 b | |
Leaf | Control | 3.97 ± 0.07 a | 4.88 ± 0.28 a | 5.66 ± 0.27 a | 6.37 ± 0.19 a |
5% PEG-6000 | 3.91 ± 0.04 a | 4.67 ± 0.11 a | 5.44 ± 0.17 a | 6.08 ± 0.22 a | |
10% PEG-6000 | 3.58 ± 0.05 b | 4.27 ± 0.23 b | 4.97 ± 0.12 b | 5.55 ± 0.21 b | |
15% PEG-6000 | 3.34 ± 0.04 c | 3.94 ± 0.22 bc | 4.60 ± 0.22 bc | 4.97 ± 0.22 c | |
20% PEG-6000 | 3.29 ± 0.04 c | 3.76 ± 0.18 c | 4.23 ± 0.28 c | 4.39 ± 0.29 d |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Qi, Y.; Ma, L.; Ghani, M.I.; Peng, Q.; Fan, R.; Hu, X.; Chen, X. Effects of Drought Stress Induced by Hypertonic Polyethylene Glycol (PEG-6000) on Passiflora edulis Sims Physiological Properties. Plants 2023, 12, 2296. https://doi.org/10.3390/plants12122296
Qi Y, Ma L, Ghani MI, Peng Q, Fan R, Hu X, Chen X. Effects of Drought Stress Induced by Hypertonic Polyethylene Glycol (PEG-6000) on Passiflora edulis Sims Physiological Properties. Plants. 2023; 12(12):2296. https://doi.org/10.3390/plants12122296
Chicago/Turabian StyleQi, Ying, Lingling Ma, Muhammad Imran Ghani, Qiang Peng, Ruidong Fan, Xiaojing Hu, and Xiaoyulong Chen. 2023. "Effects of Drought Stress Induced by Hypertonic Polyethylene Glycol (PEG-6000) on Passiflora edulis Sims Physiological Properties" Plants 12, no. 12: 2296. https://doi.org/10.3390/plants12122296