In-Site and Ex-Site Date Palm Exposure to Heavy Metals Involved Infra-Individual Biomarkers Upregulation
Abstract
:1. Introduction
2. Material and Methods
2.1. Soils and Plants Sampling from the Study Site
2.2. Ex-Situ Soil Contamination and Plant Transfer
2.3. Soil and Plant Digestion for Metal Spectroscopic Analysis and Data Processing
2.4. Biochemical Analysis
2.5. Real Time qPCR Amplification
2.6. Statistical Analysis
3. Results
3.1. Soil Contamination and Metal Concentrations in Field
3.2. Metal Concentrations in Plants
3.3. Accumulation of Oxidants in Plant under HMs Stress
3.4. Enzymatic Antioxidant and Secondary Metabolites Potential in Date Palm under HMs Stress
3.5. Profiling of HMs Related Gene Transcripts in Date Palm
4. Discussion
4.1. Metals Concentration and Their Interactions with Plant Nutrients
4.2. Impact of Non-Essential Metal Uptake on Biochemical Integrative Biomarkers in Date Palm
4.3. Essential Metal Uptake Effects on Biochemical Integrative Biomarkers in Date Palm
4.4. Infra-Individual Expression Biomarkers for HMs Stress on Date Palm
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Doped Soils | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Uncontaminated Site | Contaminated Site | Cd (mg kg−1) | Cu (mg kg−1) | Cr (mg kg−1) | |||||||||||||||
Cd | Cu | Cr | Cd | Cu | Cr | 0 | 10 | 50 | 100 | 0 | 50 | 100 | 500 | 0 | 50 | 100 | 500 | ||
Metal Concentration (mg kg−1) | Soil | 0.3 (0.1) | 5.4 (0.4) | 0.1 (0.1) | 0.9 (0.1) | 7.5 (0.3) | 28.0 (0.8) | 0.7aA (0.02) | 4.4aB (0.2) | 26.3aC (1.1) | 63.9aD (0.9) | 5.68aA (0.02) | 19.3aB (1.4) | 33.4aC (2.5) | 64.7aD (0.9) | 0.05aA (0.02) | 26.5aB (2.0) | 71.8aC (1.6) | 29.2aD (3.9) |
Roots | - | - | - | - | - | - | 0.2bA (0.02) | 4.5aAB (0.0) | 16.0aBC (5.04) | 27.1bC (5.1) | 4.85aA (0.02) | 17.2abB (2.9) | 18.8bB (2.1) | 19.6bB (1.9) | 0.64bA (0.02) | 9.8bB (0.6) | 15.4bB (0.8) | 25.8bC (0.2) | |
Leaves | 0.2 (0.0) | 3.8 (0.7) | 0.1 (0.8) | 1.6 (0.7) | 14.6 (2.8) | 17.9 (1.9) | 0.4cA (0.09) | 2.1aA (1.5) | 12.2bB (1.2) | 15.3abB (3.7) | 3.82bA (0.09) | 9.3bB (1.2) | 12.7bB (1.9) | 13.8bB (0.7) | 0.02aA (0.09) | 7.2bB (0.3) | 8.7bB (0.2) | 14.2cC (2.5) | |
Zn Concentration (mg kg−1) | Soil | 9.4 (0.2) | 15.4 (0.7) | 19.7aA (0.4) | 34.9aB (0.0) | 36.1aC (0.1) | 41.2aD (0.3) | 19.7aA (0.4) | 23.91aA (1.8) | 29.8aA (5.3) | 32.82aA (2.8) | 19.7aA (0.4) | 25.1aB (0.1) | 36.32aC (0.2) | 29.85aC (0.6) | ||||
Roots | - | - | 17.50bA (0.6) | 10.47bB (0.6) | 8.4aAB (2.0) | 6.6aAB (2.2) | 17.5bA (0.6) | 16.93bB (0.2) | 11.14aC (0.3) | 10.2aD (0.0) | 17.5bA (0.6) | 14.3bB (0.4) | 9.2bC (3.0) | 8.8bC (4.0) | |||||
Leaves | 14.0 (1.1) | 36.0 (1.5) | 29.3cA (0.9) | 18.9aA (1.4) | 15.5bA (0.7) | 6.3bB (0.5) | 29.3cA (0.9) | 22.8abB (2.8) | 10.87aC (0.2) | 8.85aC (0.0) | 29.3cA (0.9) | 18.4bB (0.3) | 11.81aC (4.0) | 10.82aC (0.1) |
Cd (mg kg−1) | Cu (mg kg−1) | Cr (mg kg−1) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
0 | 10 | 50 | 100 | 0 | 50 | 100 | 500 | 0 | 50 | 100 | 500 | |
BAF | 0.64 (0.0) | 0.74 (0.2) | 0.46 (0.1) | 0.24 (0.1) | 0.29 (0.0) | 0.49 (0.0) | 0.38 (0.1) | 0.21 (0.1) | 0.38 (0.0) | 0.27 (0.0) | 0.12 (0.0) | 0.04 (0.0) |
EF | 0.15 (0.4) | 4.94 (0.4) | 4.48 (0.3) | 5.87 (0.2) | 0.64 (0.0) | 1.28 (0.5) | 1.99 (0.1) | 1.39 (0.7) | 5.55 (0.4) | 0.08 (0.0) | 0.1 (0.02) | 0.3 (0.0) |
TF | 0.23 (0.) | 1.02 (0.0) | 0.60 (0.0) | 0.42 (0.1) | 1.77 (0.) | 0.9 (0.2) | 0.56 (0.0) | 0.30 (0.0) | 1.5 (0.0) | 0.90 (0.1) | 0.56 (0.0) | 0.30 (0.0) |
BCF | 0.93 (0.1) | 1.72 (0.0) | 1.07 (0.0) | 0.66 (0.1) | 0.19 (0.1) | 1.37 (0.1) | 0.98 (0.3) | 0.51 (0.0) | 1.89 (0.1) | 0.37 (0.0) | 0.21 (0.0) | 0.08 (0.0) |
Doped Soils | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Uncontaminated Site | Contaminated Site | Cd (mg kg−1) | Cu (mg kg−1) | Cr (mg kg−1) | |||||||||||
0 | 10 | 50 | 100 | 0 | 50 | 100 | 500 | 0 | 50 | 100 | 500 | ||||
Mn Concentration (mg kg−1) | Soil | 9.0 (0.4) | 14.1 (1.8) | 40.7aA (0.3) | 42.2aB (0.3) | 44.9aC (0.0) | 44.9aC (0.1) | 40.7aA (0.3) | 41.8aB (1.5) | 44.0aAB (0.0) | 44.3aB (0.3) | 40.7aA (0.3) | 46.8aA (2.6) | 43.6aA (1.9) | 46.4aA (2.0) |
Roots | - | - | 4.20bA (0.4) | 6.2bB (0.3) | 4.8bAB (1.0) | 2.2bA (0.3) | 4.2bA (0.4) | 6.1bB (0.1) | 8.4bC (0.7) | 4.0bA (0.5) | 4.2bA (0.4) | 8.4bB (0.5) | 7.8bC (0.2) | 6.8bBC (1.1) | |
Leaves | 4.5 (0.9) | 16.5 (1.3) | 18.3cA (0.5) | 12.3cB (0.5) | 7.1bC (0.2) | 5.1cD (0.3) | 18.3cA (0.5) | 11.2bB (0.3) | 8.6bC (0.5) | 5.6bD (0.5) | 18.3cA (0.5) | 12.3bB (0.4) | 8.9bC (0.0) | 7.1bD (0.2) | |
Pairwise Correlation coefficient (r) | |||||||||||||||
Metal concentration-Mn concentration | 0.914191 | −0.611946 | 0.914191 | −0.48363 | 0.914191 | −0.33352 |
H2O2 µmol/g FW | TBARS nmol/g FW | CAT µmole H2O2/mg Protein | APX | SOD U/mg Protein | Polyphenol % | Flavonoid % | Tannins % | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Root | Leaves | Root | Leaves | Root | Leaves | Root | Leaves | Root | Leaves | Root | Leaves | Root | Leaves | Root | Leaves | ||
0 | 156.1aA (4.4) | 244.6bA (5.8) | 44.9aA (2.9) | 56.5bA (4.0) | 1.6aA (0.0) | 1.7aA (0.2) | 8.7aA (0.2) | 9.9bA (0.6) | 444.1aA (6.2) | 550.1bA (28.7) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | |
Cd (mg kg−1) | 10 | 270.4aB (1.3) | 381.9bB (22.0) | 62.5aB (4.7) | 70.4bAB (0.7) | 2.7aB (0.1) | 3.5bB (0.0) | 12.5aB (0.1) | 13.8bB (0.2) | 590.3aB (9.3) | 612.3bB (8.5) | 114.8aB (4.7) | 153.2bB (3.8) | 201.5aB (3.3) | 201.1aB (6.1) | 132.4aB (4.19) | 113.8bB (3.4) |
50 | 568.2aC (12.2) | 599.6bC (1.7) | 94.1aC (4.1) | 86.8aC (9.7) | 1.5aA (0.0) | 1.8bC (0.0) | 12.7aB (0.2) | 14.7bB (0.1) | 540.0aB (13.0) | 542.8aA (28.1) | 134.6aC (1.4) | 145.0aB (10.6) | 186.3aB (9.8) | 205.2aB (6.5) | 109.5aA (5.8) | 150.0bC (5.4) | |
100 | 709.5aD (18.7) | 598.5bC (6.5) | 98.3aC (3.0) | 82.2bBC (2.7) | 0.9aC (0.0) | 0.4bD (0.0) | 6.1aB (0.1) | 11.4bC (0.7) | 310.6aC (10.0) | 373.6bC (22.4) | 72.0aD (4.3) | 83.7bC (2.8) | 85.4aC (5.9) | 60.6bC (8.0) | 55.8aC (4.2) | 64.9bD (1.7) | |
One-way and Two-way ANOVA main effects | |||||||||||||||||
Tissues | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | |||||||||
Concentration | 0.000 * | 0.626 | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.520 | 0.000 * | |||||||||
Tissues x Concentration | 0.000 * | 0.000 * | 0.002 * | 0.003 * | 0.001 * | 0.000 * | 0.000 * | 0.000 * | |||||||||
Cu (mg kg−1) | 0 | 156.1aA (4.4) | 244.6bA (5.8) | 44.9aA (2.9) | 56.5bA (4.0) | 1.6aA (0.0) | 1.7aA (0.2) | 8.7aA (0.2) | 9.9bA (0.6) | 444.1aA (6.2) | 550.1bA (28.7) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) |
50 | 202.9aB (4.6) | 248.4aA (20.9) | 44.3aA (3.4) | 63.5bAB (3.1) | 2.0aB (0.1) | 2.0aAB (0.1) | 10.9aB (0.1) | 11.8bB (0.2) | 583.8aB (13.8) | 633.5bB (17.7) | 116.7aA (7.0) | 127.2aB (3.8) | 238.1aA (6.6) | 247.8aB (1) | 148.4aB (7) | 151.8aB (6) | |
100 | 319.4aC (17.8) | 325.0aB (25.0) | 53.5aA (2.31) | 69.2bBC (3.17) | 2.4aC (0.1) | 2.4bBC (0.0) | 12.5aBC (0.1) | 13.7bC (0.2) | 660.8aC (12.3) | 717.1bC (9.5) | 151.5aB (6.3) | 185.2bC (12.5) | 271.2aA (18.1) | 184.8bC (11.4) | 162.6aC (6.5) | 179.8bC (2.8) | |
500 | 429.1aD (20.2) | 464.3aC (15.4) | 79.5aB (7.5) | 88.1aC (0.8) | 2.3aBC (0.2) | 2.8bC (0.1) | 11.9aC (1.4) | 14.1aC (0.1) | 829.6aB (5.5) | 776.0bC (11.) | 100.8aA (12.8) | 110.2aAB (7.68) | 223.7aA (8.2) | 94.5bA (12.9) | 87aA (4.3) | 119.7bD (6.2) | |
One-way and Two-way ANOVA main effects | |||||||||||||||||
Tissues | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.613 | 0.000 * | |||||||||
Concentration | 0.015 * | 0.000 * | 0.340 * | 0.001 * | 0.000 * | 0.990 | 0.539 | 0.004 * | |||||||||
Tissues x Concentration | 0.352 | 0.175 | 0.780 | 0.453 | 0.350 | 0.363 | 0.648 | 0.034 * | |||||||||
Cr (mg kg−1) | 0 | 156.1aA (4.4) | 244.6bA (5.8) | 44.9aA (2.9) | 56.5bA (4.0) | 1.6aA (0.0) | 1.7aA (0.2) | 8.7aA (0.2) | 9.9bA (0.6) | 444.1aA (6.2) | 550.1bA (28.7) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) | 100aA (0.00) |
50 | 183.9aA (18.1) | 268.5bA (16.0) | 45.1aA (1.9) | 59.0bA (1.9) | 2.1aAB (0.0) | 1.8aAB (0.1) | 11.0aB (0.1) | 11.4bB (0.1) | 503.5aB (7.6) | 576.0bA (9.7) | 120.2aB (1.2) | 152.6bB (2.6) | 152.5aB (3.6) | 130.7bB (1.3) | 169.6aB (3.5) | 153.0bB (5.9) | |
100 | 317.7aB (3.2) | 347.9aB (32.7) | 60.6aB (7.5) | 72.6aB (2.4) | 2.4aBC (0.3) | 2.2aBC (0.1) | 12.6aC (0.3) | 12.2aB (0.2) | 617.1aC (26.8) | 684.5bB (11.1) | 160.6aC (6.7) | 217.8bC (0.4) | 272.8aC (8.3) | 210.2bC (8.9) | 127.0aC (3.5) | 181.3bC (6.2) | |
500 | 589.4aC (11.7) | 520.9bC (27.6) | 83.0aC (2.7) | 87.1aC (0.8) | 2.7aC (0.2) | 2.3aC (0.1) | 14.5aD (0.4) | 14.5aC (0.3) | 730.7aD (11.6) | 787bC (16.6) | 130.1aD (1.0) | 113.6bD (9.4) | 199.00aA (4.1) | 151.6bA (1.5) | 103.5aA (3.3) | 112.4aA (6.7) | |
One-way and Two-way ANOVA main effects | |||||||||||||||||
Tissues | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | 0.000 * | |||||||||
Concentration | 0.229 | 0.005 * | 0.065 | 0.154 | 0.001 * | 0.342 | 0.519 | 0.177 | |||||||||
Tissues x Concentration | 0.022 * | 0.440 | 0.219 | 0.149 | 0.640 | 0.029 * | 0.900 | 0.470 |
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Chaâbene, Z.; Rorat, A.; Kriaa, W.; Rekik, I.; Mejdoub, H.; Vandenbulcke, F.; Elleuch, A. In-Site and Ex-Site Date Palm Exposure to Heavy Metals Involved Infra-Individual Biomarkers Upregulation. Plants 2021, 10, 137. https://doi.org/10.3390/plants10010137
Chaâbene Z, Rorat A, Kriaa W, Rekik I, Mejdoub H, Vandenbulcke F, Elleuch A. In-Site and Ex-Site Date Palm Exposure to Heavy Metals Involved Infra-Individual Biomarkers Upregulation. Plants. 2021; 10(1):137. https://doi.org/10.3390/plants10010137
Chicago/Turabian StyleChaâbene, Zayneb, Agnieszka Rorat, Walid Kriaa, Imen Rekik, Hafedh Mejdoub, Franck Vandenbulcke, and Amine Elleuch. 2021. "In-Site and Ex-Site Date Palm Exposure to Heavy Metals Involved Infra-Individual Biomarkers Upregulation" Plants 10, no. 1: 137. https://doi.org/10.3390/plants10010137