Impact of Immunoglobulin M-Type Donor-Specific Human Leukocyte Antigen–Antibody Levels in Supernatants from Cultured Peripheral Blood Mononuclear Cells as Predictors of Antibody-Mediated Rejection
Abstract
:1. Introduction
2. Results
2.1. Participants Characteristics
2.2. Isotypes (IgG or IgM) of DSAs Produced from PBMC Culture Supernatants Reflect the Activation Level of Humoral Immune Responses against Donor-Specific Antigen; Several IgM DSAs May Be a Precursor of the Production of IgG DSAs in the Patient Sera
2.3. IgM-Secreting mBCs Are More Sensitive to the Standard Immunosuppressive Therapy Than IgG-Secreting mBCs
3. Discussion
4. Materials and Methods
4.1. Isolation and Culture of PBMCs
4.2. Reagents
4.3. Detection of HLA Antibodies in the Culture Supernatants
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameter | Graft function Stable (N = 10) | AMR Confirmed (N = 7) | Non-DSA Sensitized (N = 12) | HLA Non-Sensitized (N = 10) | p-Value | |
---|---|---|---|---|---|---|
Gender (Male/Female) | N (%) | 4(40%)/6(60%) | 5(71.4%)/2(28.6%) | 6(50%)/6(50%) | 5(50%)/5(50%) | N.S |
Age at transplant (Year) | Mean ± SD | 48.8 ± 9.6 | 41.6 ± 19.0 | 49.8 ± 11.4 | 36.8 ± 15.9 | N.S |
ABO compatibility (Compatible/Incompatible) | N (%) | 10(100%)/0(0%) | 7(100%)/0(0%) | 11(91.7%)/1(8.3%) | 9(90.0%)/1(10%) | N.S |
Immunosuppression | ||||||
Maint. Pred (Yes/No) | N (%) | 10(100%)/0(0%) | 7(100%)/0(0%) | 11(91.7%)/1(8.3%) | 7(70%)/3(30%) | N.S |
1 = Tac, 2 = CyA | N (%) | 10(100%)/0(0%) | 7(100%)/0(0%) | 12(100%)/0(0%) | 10/(100%)/0(0%) | N.S |
1 = MMF, 2 = MZ | N (%) | 10(100%)/0(0%) | 7(100%)/0(0%) | 12(100%)/0(0%) | 10/(100%)/0(0%) | N.S |
Kidney biopsy | ||||||
g + ptc | Mean ± SD | 0.83 ± 0.69 | 3.5 ± 1.0 | <0.001 *** | ||
C4d (0–3) | Mean ± SD | 0.33 ± 0.75 | 2.0 ± 1.22 | <0.05 * |
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Imamura, R.; Matsuda, Y.; Tsutahara, K.; Nonomura, N.; Takahara, S. Impact of Immunoglobulin M-Type Donor-Specific Human Leukocyte Antigen–Antibody Levels in Supernatants from Cultured Peripheral Blood Mononuclear Cells as Predictors of Antibody-Mediated Rejection. Pathogens 2020, 9, 733. https://doi.org/10.3390/pathogens9090733
Imamura R, Matsuda Y, Tsutahara K, Nonomura N, Takahara S. Impact of Immunoglobulin M-Type Donor-Specific Human Leukocyte Antigen–Antibody Levels in Supernatants from Cultured Peripheral Blood Mononuclear Cells as Predictors of Antibody-Mediated Rejection. Pathogens. 2020; 9(9):733. https://doi.org/10.3390/pathogens9090733
Chicago/Turabian StyleImamura, Ryoichi, Yoshiko Matsuda, Koichi Tsutahara, Norio Nonomura, and Shiro Takahara. 2020. "Impact of Immunoglobulin M-Type Donor-Specific Human Leukocyte Antigen–Antibody Levels in Supernatants from Cultured Peripheral Blood Mononuclear Cells as Predictors of Antibody-Mediated Rejection" Pathogens 9, no. 9: 733. https://doi.org/10.3390/pathogens9090733