SARS-CoV-2 Nucleocapsid Protein Mutations Found in Switzerland Disrupt N-Gene Amplification in Commonly Used Multiplex RT-PCR Assay
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Samples
2.2. Data Collection and Analysis
2.3. GISAID
3. Results
3.1. Characteristics
3.2. Genomic Analysis
3.3. Target Failure as a Result of Concurrent Mutation Presence
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Mutation | Amino Acid | Effect on Assay | Assay | References |
---|---|---|---|---|
G29179T | synonymous | partial NGTF | Cepheid Xpert Xpress SARS-CoV-2 | [24,25,26,27] |
C29197T | synonymous | NGTF | Cepheid Xpert Xpress SARS-CoV-2 | [28,29,30,31] |
C29200T | synonymous | NGTF | Cepheid Xpert Xpress SARS-CoV-2 | [11,28,29,30] |
28,913–28,915 | G214del | NGTF | TaqPath COVID-19 Combo Kit | [20] |
28,916–28,918 | G215del | NGTF | TaqPath COVID-19 Combo Kit | [20] |
28,913–28,918 | G214–G215 deletion | NGTF | AllplexTM SARS-CoV-2 Assay, Seegene | [10] |
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PANGO Lineage | # | Frequency (%) | CT (median) | G215C + A217S | G214- | G214/215- | Del207–219 |
---|---|---|---|---|---|---|---|
AY.43 | 8 | 5.4 | 24.8 | 8 | |||
AY.33 | 2 | 1.3 | 21.8 | 2 | |||
AY.4 | 102 | 68.5 | 25.7 | 99 | 3 | ||
B.1.617.2 | 3 | 2.0 | 26.0 | 2 | 1 | ||
AY.4.3 | 4 | 2.7 | 23.9 | 4 | |||
AY.46.6 | 1 | 0.7 | 21.8 | 1 | |||
AY.125 | 11 | 7.4 | 23.4 | 11 | |||
AY.98.1 | 5 | 3.4 | 25.4 | 5 | |||
AY.36 | 9 | 6.0 | 25.4 | 9 | |||
AY.122 | 1 | 0.7 | 25.6 | 1 | |||
AY.43.4 | 2 | 1.3 | 22.5 | 2 | |||
BA.2 | 1 | 0.7 | 21.8 | 1 | |||
na 1 | 21 | 28.8 | 18 | 2 | 1 | ||
Total | 170 | 100.0% | 151 | 8 | 10 | 1 |
GISAID VOC | N_A217S | Total | Frequency |
---|---|---|---|
Alpha | 193 | 1,188,205 | 0.016% |
Beta | 7 | 43,474 | 0.016% |
Gamma | 22 | 126,630 | 0.017% |
Delta | 3518 | 4,459,248 | 0.079% |
Omicron | 698 | 4,588,964 | 0.015% |
GISAID VOC | N_A217S | N_G215C and N_A217S |
---|---|---|
Alpha | 214 | 0 |
Beta | 7 | 0 |
Gamma | 22 | 0 |
Delta | 3651 | 3515 |
Omicron | 1358 | 3 |
Total | 6020 | 3518 |
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Hilti, D.; Wehrli, F.; Roditscheff, A.; Risch, M.; Risch, L.; Egli, A.; Bodmer, T.; Wohlwend, N. SARS-CoV-2 Nucleocapsid Protein Mutations Found in Switzerland Disrupt N-Gene Amplification in Commonly Used Multiplex RT-PCR Assay. Pathogens 2023, 12, 1383. https://doi.org/10.3390/pathogens12121383
Hilti D, Wehrli F, Roditscheff A, Risch M, Risch L, Egli A, Bodmer T, Wohlwend N. SARS-CoV-2 Nucleocapsid Protein Mutations Found in Switzerland Disrupt N-Gene Amplification in Commonly Used Multiplex RT-PCR Assay. Pathogens. 2023; 12(12):1383. https://doi.org/10.3390/pathogens12121383
Chicago/Turabian StyleHilti, Dominique, Faina Wehrli, Anna Roditscheff, Martin Risch, Lorenz Risch, Adrian Egli, Thomas Bodmer, and Nadia Wohlwend. 2023. "SARS-CoV-2 Nucleocapsid Protein Mutations Found in Switzerland Disrupt N-Gene Amplification in Commonly Used Multiplex RT-PCR Assay" Pathogens 12, no. 12: 1383. https://doi.org/10.3390/pathogens12121383