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Article

Design, Synthesis and Evaluation of Praziquantel Analogues and New Molecular Hybrids as Potential Antimalarial and Anti-Schistosomal Agents

by
Freddy Mugisho Kasago
1,
Cécile Häberli
2,
Jennifer Keiser
2 and
Wayiza Masamba
1,*
1
Department of Chemical and Physical Sciences, Faculty of Natural Sciences, Walter Sisulu University, Nelson Mandela Drive, Mthatha 5117, South Africa
2
Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Kreuzstr. 2, CH-4123 Allschwil, Switzerland
*
Author to whom correspondence should be addressed.
Molecules 2023, 28(13), 5184; https://doi.org/10.3390/molecules28135184
Submission received: 24 May 2023 / Revised: 28 June 2023 / Accepted: 29 June 2023 / Published: 3 July 2023
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Abstract

:
Malaria and schistosomiasis are two of the neglected tropical diseases that persistently wreak havoc worldwide. Although many antimalarial drugs such as chloroquine are readily available, the emergence of drug resistance necessitates the development of new therapies to combat this disease. Conversely, Praziquantel (PZQ) remains the sole effective drug against schistosomiasis, but its extensive use raises concerns about the potential for drug resistance to develop. In this project, the concept of molecular hybridization was used as a strategy to design the synthesis of new molecular hybrids with potential antimalarial and antischistosomal activity. A total of seventeen molecular hybrids and two PZQ analogues were prepared by coupling 6-alkylpraziquanamines with cinnamic acids and cyclohexane carboxylic acid, respectively. The synthesised compounds were evaluated for their antimalarial and antischistosomal activity; while all of the above compounds were inactive against Plasmodium falciparum (IC50 > 6 µM), many were active against schistosomiasis with four particular compounds exhibiting up to 100% activity against newly transformed schistosomula and adult worms at 50 µM. Compared to PZQ, the reference drug, the activity of which is 91.7% at 1 µM, one particular molecular hybrid, compound 32, which bears a para-isopropyl group on the cinnamic acid moiety, exhibited a notable activity at 10 µM (78.2% activity). This compound has emerged as the front runner candidate that might, after further optimization, hold promise as a potential lead compound in the fight against schistosomiasis.

1. Introduction

In 2021, the World Health Organisation (WHO) reported 240 million schistosomiasis cases worldwide with 700 million people living in regions classified as endemic [1,2], a real health threat in developing countries. Humans are infected by contact with contaminated freshwater, either by swimming or working in infected water bodies. Once inside the human body, the adult worms settle in the blood vessels and can live there for years, undetected by the immune system while excreting hundreds to thousands of eggs daily, which either leave the body in excreta or become trapped in nearby tissues [3,4,5]. The disease can lead to a range of complications, including liver and spleen damage, kidney failure, bladder cancer, and increased susceptibility to other infections, as well as death if left untreated [6].
Efforts to control and eliminate the disease in Africa and the world have been ongoing for many years. These include prevention measures by improving sanitation and hygiene, and using medication to treat infected individuals [7]. In the absence of any vaccine against schistosomiasis, chemotherapy has remained the only option for its control. Praziquantel (PZQ) [8] emerged as the only drug amongst many compounds screened for their anthelmintic activity. The drug is typically administered as a racemate; however, the active enantiomer, (R)-PZQ, exhibits reduced side effects and can be obtained through either classical resolution [9] or asymmetric synthesis [9,10]. Due to its efficacy, safety, operational convenience, and low cost, this drug is extensively and exclusively used today with no viable alternative. Unfortunately, this is a recipe for the development of the phenomenon of resistance [11].
Despite its limitations, such as its efficacy on only 1–2-week-old larvae and 5-week and older adult worms, and its inability to kill immature worms 3–4 weeks post infection, PZQ can still serve as a valuable scaffold for molecular hybridization in the development of new lead compounds to combat schistosomiasis.
On the other hand, malaria, a life-threatening disease caused by Plasmodium parasites transmitted through the bites of infected mosquitoes, continues to be a significant global health concern. Current treatments for malaria primarily rely on artemisinin-based combination therapies (ACTs) [12], which combine artemisinin derivatives with other antimalarial drugs. While ACTs have proven effective in reducing mortality rates, the emergence and spread of drug-resistant strains of Plasmodium pose a major challenge [13]. The development of resistance to artemisinin in Southeast Asia and the potential for its spread to other regions underscores the urgent need for novel antimalarial drugs. Additionally, ensuring the accessibility and affordability of these treatments in resource-limited settings remains a critical obstacle in combating malaria worldwide. Mefloquine (see Figure 1) is an antimalarial drug structurally related to quinine with high worm burden reduction (93%) in the S. mansoni mouse model [14]. Given the similarities between the PZQ basic structure with the antimalarial quinoline and isoquinoline pharmacophores, two well-known malaria drug scaffolds, it was anticipated that the modified PZQ analogues and molecular hybrids would be active against Plasmodium falciparum in addition to their expected antischistosomal activity.
Molecular hybridization as a drug discovery strategy involves the rational design of new chemical entities by the fusion (usually via a covalent linker) of two or more drugs, both active compounds and/or pharmacophoric units recognised and derived from known bioactive molecules [15,16,17]. The expected outcome for this chemical modification is to produce a new hybrid compound with improved affinity and efficacy, when compared to the parent drugs. Additionally, this strategy can result in compounds presenting a modified selectivity profile, different and/or dual modes of action, and reduced undesired side effects, ultimately, leading to new therapies.
Molecular hybrids in their general form represent intra-molecular combined therapies where the previously independent biologically active principles that act at different targets are brought together by a covalent link in order to combine the effects of each molecule and address the complexity deriving from a plethora of biochemical and physiological processes involved in a particular pathology [15,18,19]. This concept of hybridization of biologically active molecules has been used by numerous research groups to target a variety of diseases including cancer, malaria, tuberculosis, and HIV/AIDS, as well as schistosomiasis, amongst others [19,20,21].
Based on precedents in the literature [22], molecular hybridization is expected to accelerate the discovery of new lead compounds for the treatment of schistosomiasis.
Cinnamic acid and derivatives have demonstrated a wide range of biological activities in different studies and had been reported to have significant activity against malaria parasites [23]. Their incorporation in the PZQ tetrahydroisoquinoline nucleus, a well-known malaria scaffold, is anticipated to confer antimalarial activity to these new molecular entities. Indeed, several chemical entities with isoquinoline structure isolated from medicinal plants have been reported to demonstrate good antiplasmodial activity comparable to chloroquine taken as a reference [24].
The work of Liu et al. has shown that substitution on position C3 (see Figure 1) of PZQ maintains the two aromatic rings in a non-coplanar conformation, which leads to a complete loss of biological activity. Hence, the substitution at C6, which so far has remained unexplored, might contribute to the overall optimum activity by maintaining the coplanarity of the cinnamic acid moiety with the other aromatic ring.
Molecules 28 05184 g001 550
Figure 1. Chemical structure of PZQ including atom numbering (in red, the chiral center of the molecule [25] and the structure of mefloquine.
Figure 1. Chemical structure of PZQ including atom numbering (in red, the chiral center of the molecule [25] and the structure of mefloquine.
Molecules 28 05184 g001
The initial synthesis of praziquantel was achieved by Merck, starting with the Reissert reaction of the readily available isoquinoline core 1, followed by a high-pressure catalytic hydrogenation step to generate an amine intermediate 3. The subsequent acylation of the latter with chloroacetyl chloride followed by a base-catalyzed cyclisation resulted in the formation of the target compound 5 [26]. The synthetic pathway is depicted in Scheme 1 below:
This approach was found to be limited in two aspects: firstly, the Reissert reaction, the initial step of the process, could only be carried out with a restricted number of acid chlorides, and secondly, the catalytic hydrogenation of 3 requires high pressure (70 atm) [27] in order to generate a 1-aminomethyltetrahydroisoquinoline intermediate [26].
The merit of the Merck approach is the use of inexpensive and readily available raw materials. For this reason, it is still widely used for the large-scale production of PZQ [28]. In addition, the use of toxic cyanide is environmentally unfriendly and the overall yield of the reaction has not been reported.
A highly efficient method for the synthesis of PZQ derivatives using a two-step, one-pot procedure that involves a four-component Ugi reaction followed by the Pictet–Spengler reaction was described by Liu et al. [29,30] (Scheme 2). This approach offers significant advantages in achieving a wide range of structural diversity, thanks to the extensive availability of commercially accessible carboxylic acids and aldehydes. However, while elegant and convergent, the broader applicability of this approach is hindered by the limited availability of the required isocyanides 6.
A third widely used strategy for the preparation of PZQ is outlined in Scheme 3 [31]. This approach involves a three-step synthesis of piperazinone 21 (R = H), where the formation of both the piperazine and isoquinoline rings simultaneously occurs in a one-pot procedure. By acylating with cyclohexanecarbonyl chloride, the desired final product is obtained. One notable advantage of this synthetic pathway is its ability to introduce molecular diversity through the acylating agent. As a result, numerous alternative methods that rely on the formation of the tetrahydroisoquinoline core have emerged in the literature [30,32].
The primary objective of this project is to create new molecular hybrids by combining the two aforementioned scaffolds, with the aim of discovering potential treatments for schistosomiasis.

2. Results

2.1. Chemistry

An initial attempt was undertaken to utilize the four-component Ugi reaction, starting from amphetamine-derived isonitrile 6a (Ar = Ph, R1 = Me), formaldehyde 8a (R2 = H), trans-cinnamic acid 9a (R3 = PhCH = CH-), and aminoacetaldehyde dimethyl acetal 7, proved unsuccessful as the anticipated product was not detected after the reaction. This outcome can be attributed to the limited solubility of paraformaldehyde within the given reaction conditions (methanol, 0 °C). As a result, our focus was redirected toward the approach by Kim et al. [31].
The key intermediates in this strategy, 6-Alkylpraziquanamines 21a,b (R = Me and Et), were prepared using a two-step procedure involving the condensation of benzaldehyde 12 with n-butylamine 13 to generate the corresponding Schiff base 14 (benzaldehyde imine), followed by reaction with the appropriate nitroalkane (nitroethane 15a and 1-nitropropane 15b, respectively) to obtain the expected nitroamines in an aza-Henry reaction (Scheme 4). The resulting nitroamines were not isolated and underwent intramolecular deamination under acidic conditions to yield 2-nitro-1-phenylalkenes intermediates 16a,b [33]. The two anticipated nitroalkenes 16 were obtained as trans-isomers, known for their greater thermodynamic stability than their cis-isomers, as evidenced by the chemical shifts of their olefinic protons (8.09 ppm for [(1E)-2-nitroprop-1-en-1-yl]benzene 16a and 8.00 ppm for [(1E)-2-nitrobut-1-en-1-yl]benzene 16b) (see Scheme 4). Although the Schiff base formation is a straightforward, high-yielding reaction step, the overall moderate yield of 52.6–59.9% in this two-step process can be attributed to the extensive polymerization of the highly reactive nitroalkenes under the reaction conditions. Indeed, a considerable amount of resinous materials and decomposition products were formed during this reaction. In order to synthesize 1-phenylpropan-2-amine (amphetamine) 17a and 1-phenylbutan-2-amine 17b, which are the methyl and ethyl analogues of phenylethylamine, respectively, the aforementioned nitroalkenes 16 underwent a reduction implementing the procedure described by Collins and colleagues [26]. The use of LiAlH4 as a reducing agent resulted in moderate yields of the two corresponding amines 17 (51.1% and 65.1%). The moderate yield observed in these two reactions may be attributed to the potential formation of complexes between the amine functionality and the aluminum cation, generating a dense emulsion during the work-up process. Consequently, the extraction and recovery of the product became considerably challenging. Attempts to reduce these nitroalkenes 16 by hydrogenation over nickel (Ra-Ni) and Pt catalysts under moderate or high pressures, as well as reduction with NaBH4/Ni(OAc)2 [34], were unsuccessful. Out of all the reduction methods evaluated, only reduction using LiAlH4 in dry THF under reflux in a nitrogen atmosphere for 7 h yielded the expected products.
Access to the alkylated praziquanamine derivatives 21a,b was achieved by a three-step procedure. First, the abovementioned amines were condensed with either bromoacetyl bromide 18a (X = Br) or chloroacetyl chloride 18b (X = Cl) following the procedure of Kim et al. outlined above [31]. Then, the intermediates 19 obtained in the above reaction were reacted with aminoacetaldehyde dimethylacetal 7 to afford intermediates 20a,b. Finally, intermediates 20a,b were subjected to Pictet-Spiengler cyclisation (Scheme 3), resulting in the formation of the praziquanamine derivatives 21a,b.
The first condensation between bromoacetyl bromide 18a (X = Br) with amphetamine 17a (R = Me) led to the formation of the expected intermediate 2-bromo-N-(1-phenylpropan-2-yl)acetamide 19ac (R = Me, X = Br) in 97.6% yield. In the subsequent step, intermediate 19ac underwent an aminoalkylation reaction with aminoacetaldehyde dimethylacetal 7 to afford intermediate 2-[(2,2-dimethoxyethyl)amino]-N-(1-phenylpropan-2-yl)acetamide 20a (R = Me) in 85.7% yield. Finally, treatment of 20a with sulphuric acid produced the desired product, 6-methylpraziquanamine 21a (R = Me) in 88.4% yield.
Condensing phenylbutyl amine 17b (R = Et) with chloroacetyl chloride 18b (X = Cl) resulted in the formation of 2-chloro-N-(1-phenylbutan-2-yl)acetamide 19bd (R = Et, X = Cl) in 39.0% yield; this was followed by the aminoalkylation of 19bd with amino acetaldehyde dimethylacetal 7 to afford 2-[(2,2-dimethoxyethyl)amino]-N-(1-phenylbutan-2-yl)acetamide 20b in 68.9% yield. In the condensation of 19 with acetaldehyde dimethyl acetal 7, a minimum of two equivalents of the latter were required, one of which served as a base to neutralize the in-situ-generated acid (HBr or HCl).
Upon cleavage of the dimethoxy-protecting groups with concentrated H2SO4, the aldehyde group was released and underwent concomitant Pictet-Spengler cyclisation, ultimately yielding 6-methyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 21a (R = Me) and the 6-ethyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 21b (R = Et) in 88.4 and 35.9% yields, respectively. This intramolecular cyclisation involves the intermediary formation of a reactive iminium cation, which is attacked by the neighboring aromatic ring nucleophile (see Scheme 5).
The yield of 6-Ethyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 21b (R = Et) was lower than its methyl analogue, possibly due to the steric hindrance caused by the bulkier ethyl group compared to the methyl group.
The coupling reaction between the above 6-alkylpraziquanamine derivatives 21a,b with cyclohexanecarbonyl chloride and the relevant cinnamoyl chlorides proceeded under anhydrous conditions and afforded good yields of the new PZQ analogues (22,23) and molecular hybrids (24–40), respectively, according to Scheme 6 below:
The results obtained are disclosed in Table 1 below.

2.2. Biological Activity

The antiplasmodium activity of the above 19 compounds was assessed at the Drug Discovery and Development Centre (H3D), the University of Cape Town, while the antischistosomal activity was evaluated at the Swiss Tropical and Public Health Institute (Basel, Switzerland). The results of antiplasmodium activity are reported in the Supplementary Materials [35,36], while Table 2 below summarizes the results of the antischistosomal evaluation. At 50 µM, six compounds showed high activity (85–100%) against NTS. At a lower concentration (10 µM), these compounds revealed only low to moderate activity. The six compounds progressed into tests against adult S. mansoni. At 50 µM, four compounds revealed high activity (77–100% activity) and compound 32 also showed high activity at 10 µM. An IC50 value of 1.9 µM was determined for this compound.

3. Discussion

Condensation between 6-alkylpraziquanamines 21a,b with the relevant acyl chlorides to afford PZQ analogues 22 and 23 and molecular hybrids 24–40 was carried out under standard conditions and smoothly led to the expected compounds in good to excellent yields (see Table 1), save for molecular hybrid 30, obtained in a moderate yield (51.0%). This yield can be improved after optimization.
Contrary to our expectation, none of the nineteen molecular hybrids and PZQ analogues showed any significant activity against P. falciparum. Given the similarity between the praziquanamine core and the quinoline scaffold, based on the structure-activity relationship and since both Plasmodium and Schisotosoma parasites share the same redox-heme iron mechanism, we expected a plausible in vitro activity against both malaria and schistosomiasis. Their IC50 values are all above 6 µM (see Supplementary Materials), compared to 0.005 and 0.009 µM for artesunate and chloroquine, respectively.
These results confirm that the activity exhibited by certain antimalarial drugs, such as mefloquine, involves a mechanism different from that of the praziquanamine core [14].
Equally surprising was the low activity of PZQ analogues 22 and 23 against schistosomes with an activity of 43.3% and 75% against schistosomula for 22 and 23, respectively. The low and moderate activity of 22 and 23, moving from a methyl to an ethyl group, can be explained by precedents in the literature that demonstrated that substitution at the C6 position will affect PZQ activity, which may increase with further steric hindrance (in this case brought about by the extra methylene group). Indeed, the activity of 23 is almost double (1.7 times higher) that of 22 at 50 µM and 1.9 times higher than the activity of 22 at 10 µM.
A bromine atom substitution in the ortho position in combination with a fluorine atom in the para position, as in 31, enhances the activity (100% at 50 µM), while a chlorine atom substitution in the ortho position leads to a loss of activity (see compound 30, the activity of which stands at 63.3% at 50 µM). The para substitution seems to favour the activity; compared to 25, which bears a chlorine atom in the para position, 30 has a lower activity, while 35 exhibits a high activity of 100% at 50 µM.
Compound 36, with a nitro group on the para position of the cinnamic acid moiety, shows a high activity of 100% at 50 µM, while the nitro group on the meta position, as in 29, leads to a loss of activity.
Comparing 39 and 40, each of which contains two methoxy groups, shows that activity increases when one methoxy group is located at the meta position and the other is at the para position. Conversely, activity decreases when one methoxy group is positioned at the ortho position and the other is at the para position.
Most of the compounds that exhibit the highest level of activity, namely 32, 35, 36, and 40, all share a common feature: a para substitution. This substitution appears to be a privileged position that enhances the activity of these compounds. A fluorine atom in the para position seems to exhibit the opposite effect, decreasing the activity, as in 26 and 37, suggesting that the high activity in 31 might solely originate from the contribution of the bromine atom.
Overall, seven molecular hybrids exhibited significant in vitro activity against schistosomes (both NTS as well as adult S. mansoni).
Compound 32 showed the highest activity with an IC50 value of 1.9. In comparison, the IC50 value for the reference (PZQ) is 0.54 µM [36,37].
This compound appears to be the front runner of all the active compounds, followed by 31, 35, and 40. It can be noted that compounds 32, 35, and 40 are all derived from cinnamic acid bearing a substituent on the para position, with an isopropyl group inducing the highest activity. The above four molecular hybrids are potential new leads in the fight against schistosomiasis and will be optimised for better activity.

4. Materials and Methods

4.1. General

The progress of reactions was monitored by thin-layer chromatography (TLC) using a UV Labex lamp supplied by Tallaboratories to visualise spots on TLC silica gel 60F254 plates under short and medium wavelength light at 254 and 312 nm, respectively. The target compounds were purified by column chromatography using silicagel 60, Merck Kieselgel 60 (70–230). Eluents were prepared by mixing distilled solvents, especially n-hexane, ethyl acetate, dichloromethane (DCM), and diethylether, depending on the properties of the product. The purified products were characterised by 1HNMR and 13C-NMR spectra recorded on a 400 MHz Varian NMR spectrometer. Chemical shifts (d) are given in ppm, relative to the signal of tetramethylsilane (TMS); and coupling constants (J) are given in Hertz. IR spectra were recorded on a PerkinElmer Spectrum One; peak frequencies are given in cm−1. Mass spectrometry was determined on an Agilent GC-MS instrument (6890N, Agilent Technologies). Separation was performed on a non-polar ZB-5Ms (30 m, 0.25 mm ID, 0.25 µm film thickness) Zebron 7HG-G010-41 capillary column. Helium was used as the carrier gas at a flow rate of 1 mL/min. The injector temperature was maintained at 240 °C. An amount of 1 µL of the sample was injected in a 20:1 split ratio. The oven temperature was programmed as follows: 100 °C for 5 min, ramped to 330 °C at a rate of 20 °C/min for 10 min. Mass spectra were obtained by electron impact ionisation (EI).

4.2. In Vitro Antischistosomal Assays; Screening on Newly Transformed Schistosomula (NTS)

S. mansoni cercariae were mechanically changed to newly transformed schistosomula as described elsewhere [38]. Briefly, snails were placed under light to stimulate cercarial shedding, and the cercarial suspension was collected. The tails were separated from the heads by rinsing three times with cold HBSS. NTS were then incubated overnight in a culture medium and used the next day. Test compounds and controls were dissolved in DMSO (Fluka, Buchs, Switzerland) to a concentration of 10 mM. One hundred NTS were then incubated in each well of a 96-well plate with culture medium and the test compound for a final well volume of 250 μL. The culture medium was composed of Medium 199 (Invitrogen, Carlsbad, CA, USA) supplemented with 5% fetal calf serum (Lucerne, Switzerland) and a 1% penicillin/streptomycin mixture (Lucerne, Switzerland). Compounds were tested at 10 and 50 μM in triplicate and repeated once with NTS incubated in no more than 1% DMSO. NTS incubated in 1% DMSO and PZQ served as negative and positive controls. NTS were kept in an incubator at 37 °C and 5% CO2 for up to 72 h, after which the condition of the NTS was microscopically evaluated using a scale from 3 (normal activity and morphological alteration) to 0 (dead).

4.3. Adult S. mansoni Worms

To obtain adult schistosomes, mice were subcutaneously infected with 80–100 cercariae. The mice were then euthanised after 7–8 weeks with CO2, and the worms were collected from the hepatic portal and mesenteric veins. Two pairs of adult worms were incubated in each well of a 24-well plate with 2 mL of a culture medium and the test compound. The culture medium was composed of RPMI 1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 5% fetal calf serum (Lucerne, Switzerland) and a 1% penicillin/streptomycin mixture (Lucerne, Switzerland). Compounds with >70% activity against NTS were evaluated against adult worms at 1, 10, and 50 μM with incubation at <1% DMSO. NTS incubated in 1% DMSO and PZQ served as negative and positive controls. Worms were kept in an incubator at 37 °C and 5% CO2 for up to 72 h, after which their condition was microscopically evaluated using a scale from 3 (normal activity and no morphological alterations) to 0 (dead) [39]. All animal experiments were approved by Swiss national and cantonal authorities (permit 2070).
6-Methylpraziquanamine 21a: to 2-[(2,2-dimethoxyethyl)amino]-N-(1-phenylpropan-2-yl)acetamide (1.77 g; 6.3 mmole) was added drop wise to a solution of concentrated sulphuric acid (1.68 mL; 32.8 mmoles, 5.2 eqv) at 5 °C. After stirring at room temperature for 3.5 h, the reaction mixture was poured into ice water and the pH was adjusted to 12 with 25% aqueous NaOH solution with cooling. The mixture was extracted with ethyl acetate and the ethyl acetate solution was washed with brine, dried (Na2SO4), and concentrated to give a brown viscous oil (1.20 g; 88.4%). The product was purified by column chromatography using methanol as the mobile phase. 1H NMR (400 MHz, CDCl3) δ 7.25–7.13 (m, 2H), 7.10 (dq, J = 6.6, 3.8 Hz, 2H), 5.34–5.22 (m, 1H), 4.70 (dd, J = 9.9, 4.4 Hz, 1H), 3.71–3.55 (m, 2H), 3.48 (dd, J = 17.3, 0.8 Hz, 1H), 3.39 (t, J = 0.7 Hz, 1H), 3.07 (dd, J = 16.1, 5.8 Hz, 1H), 2.86 (ddd, J = 12.8, 9.8, 0.9 Hz, 1H), 2.57–2.48 (m, 1H), 1.09 (d, J = 6.9 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 166.87, 132.94, 132.54, 129.91, 127.05, 126.31, 124.34, 52.59, 49.91, 49.48, 41.20, 34.14, 16.44. FT-IR (cm−1): 2936.69, 1647.96, 1510.48, 1469.39, 1139.65, 1046.87, 741.74, 711.81.
6-Ethylpraziquanamine 21b: was obtained using a similar procedure as that of 6-methylpraziquanamine, starting from the corresponding N-[(2-phenyl)ethyl[(2,2-dimethoxyethyl)amino] acetamide (1.76 g), and yielded a brown viscous oil (0.49 g; 35,9%). The product was purified by column chromatography (on alumina) with n-hexane and ethyl acetate mixture (2:1) as eluent; 1H NMR (400 MHz, CDCl3) δ 7.28–7.09 (m, 4H), 5.15–5.05 (m, 1H), 4.67 (dd, J = 10.1, 4.3 Hz, 1H), 3.74–3.65 (m, 2H), 3.56 (d, J = 17.5 Hz, 1H), 3.08 (dd, J = 16.3, 5.8 Hz, 1H), 2.88 (dd, J = 12.9, 10.0 Hz, 1H), 2.65 (dd, J = 16.3, 1.9 Hz, 1H), 1.97–1.90 (m, 1H), 1.44 (m, 2H), 0.91 (t, J = 7.4 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 167.37, 133.20, 132.89, 130.03, 127.12, 126.40, 124.58, 53.19, 50.13, 49.97, 47.06, 32.42, 23.25, 10.70. FT-IR (cm−1): 2921.67; 1633.86; 1495.46; 1454.35; 1305.48; 1124.61; 1030.84; 739.64; 699.73.
General procedure for the synthesis of praziquantel analogues and molecular hybrids: the coupling process was performed following a general procedure whereby cyclohexanecarbonyl chloride or the corresponding cinnamoyl chlorides were condensed with 6-alkylpraziquanamines. The acyl chlorides were prepared in a separate flask by the dropwise addition of thionyl chloride to a mixture of the corresponding acid in dry DCM. When the TLC showed that the reaction was complete, the solvent was evaporated, including excess thionyl chloride, and the residue was redissolved in 1 millilitre of dry dichloromethane. The resulting solution was added to a mixture of potassium carbonate (1.1 eqv) and the substrate (6-alkylpraziquanamine, 1–5 mmoles) in 2 mL of dry dichloromethane. The solution was stirred at RT until the TLC showed that the reaction was complete. The product was then filtered off, evaporated, and purified by column chromatography.
2-(Cyclohexylcarbonyl)-6-methyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one, 6-methylpraziquantel 22; colourless crystals (0.10 g, 68.3%); m.p. 133–135 °C; Rf = 0.365 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.23–7.25 (m, 4H), 5.22 (s, 1H), 4.96 (d, J = 13.7 Hz, 1H), 4.71 (d, J = 10.0 Hz, 1H), 4.45–4.36 (m, 1H), 4.10 (d, J = 17.3 Hz, 1H), 3.12 (dd, J = 16.0, 5.7 Hz, 1H), 3.00–2.89 (m, 1H), 2.60 (d, J = 16.1 Hz, 1H), 2.45 (t, J = 12.0 Hz, 1H), 1.58–1.44 (m, 2H), 1.25 (d, J = 11.0 Hz, 7H), 1.14–1.04 (m, 4H). 13C NMR (101 MHz, CDCl3) δ 174.84, 164.18, 132.49, 131.80, 129.91, 127.68, 126.83, 125.28, 51.53, 49.00, 45.10, 42.50, 40.78, 34.29, 29.09, 28.97, 25.67, 16.96. FT-IR (cm−1): 3748.29, 2925.97, 2007.79, 1735.02, 1623.12, 1435.91, 1327.90, 1225.25, 759.60. GC-MS (EI): tR = 15.747 min, MW = 326.4, m/z[M+] = 326.2.
2-Cyclohexanecarbonyl-6-ethyl-1,2,3,6,7,11b-hexahydro-pyrazino[2,1-a]isoquinolin-4-one, 6-ethylpraziquantel 23; yellow crystals (0.10 g, 64.2%); Rf = 0.483 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.27–7.24 (m, 4H), 7.14 (d, J = 6.8 Hz, 2H), 5.06 (d, J = 14.6 Hz, 2H), 4.68 (d, J = 10.9 Hz, 1H), 4.47 (d, J = 17.4 Hz, 1H), 4.11 (d, J = 17.5 Hz, 1H), 3.10 (dd, J = 16.3, 5.9 Hz, 1H), 2.87 (dd, J = 13.4, 10.4 Hz, 1H), 2.70 (d, J = 16.1 Hz, 1H), 2.47 (t, J = 11.9 Hz, 1H), 1.96 (s, 2H), 1.58–1.51 (m, 1H), 1.44 (tt, J = 14.3, 6.6 Hz, 1H), 1.34–1.21 (m, 6H), 0.89 (dt, J = 17.6, 7.4 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 174.79, 164.60, 132.62, 131.94, 129.88, 127.59, 126.77, 125.32, 51.78, 49.07, 48.15, 45.31, 40.78, 32.36, 29.26, 28.92, 25.71, 25.64, 10.65. FT-IT (cm−1): 2927.16, 2848.30, 1650.76, 1634.25, 1411.13, 1317.89, 1211.14, 1069.25, 759.32, 725.04. GC-MS (EI): tR = 15.989 min, MW = 340.4, m/z[M+] = 340.2.
6-Methyl-2-[(2E)-3-phenylprop-2-enoyl]-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 24; yellow crystals (0.12 g, 73.4%); m.p. 96–101 °C; Rf = 0.30 (diethyl ether); 1H NMR (400 MHz, CDCl3) δ 7.76 (d, J = 15.4 Hz, 1H), 7.55–7.52 (m, 2H), 7.41–7.37 (m, 3H), 7.35 (s, 1H), 7.33–7.22 (m, 2H), 7.17 (s, 1H), 6.82 (d, J = 15.3 Hz, 1H), 5.26 (s, 1H), 5.09 (d, J = 13.3 Hz, 1H), 4.82 (s, 1H), 4.58 (d, J = 17.6 Hz, 1H), 4.25 (d, J = 17.1 Hz, 1H), 3.19–3.03 (m, 2H), 2.62 (d, J = 16.0 Hz, 1H), 1.12 (d, J = 6.9 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 165.18, 163.92, 144.38, 134.77, 132.49, 131.60, 130.12, 129.96, 128.91, 127.98, 127.75, 126.90, 125.49, 115.89, 51.93, 49.14, 45.73, 42.47, 34.40, 16.87. FT-IR (cm−1): 3856, 3358, 2937, 1413, 1204, 974, 761. GC-MS (EI): tR = 17.362 min, MW = 346.4, m/z[M+] = 346.2.
2-[(2E)-3-(4-Chlorophenyl)prop-2-enoyl]-6-methyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 25: light yellow solid (0.17 g, 93.6%); m.p. 196–200 °C (dec.); Rf = 0,47 (DCM-ethyl acetate; 1:1.5); 1H NMR (400 MHz, CDCl3) δ 7.71 (d, J = 15.3 Hz, 1H), 7.47 (d, J = 8.3 Hz, 2H), 7.41–7.32 (m, 2H), 7.32–7.25 (m, 3H), 7.17 (d, J = 7.1 Hz, 1H), 6.81 (d, J = 15.3 Hz, 1H), 5.25 (s, 1H), 5.06 (d, J = 13.3 Hz, 1H), 4.81 (d, J = 9.6 Hz, 1H), 4.57 (d, J = 17.2 Hz, 1H), 4.25 (d, J = 17.2 Hz, 1H), 3.18–3.01 (m, 2H), 2.67–2.58 (m, 1H), 1.12 (d, J = 6.9 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 164.86, 163.53, 142.75, 135.82, 133.15, 132.37, 129.91, 129.06, 129.04, 127.69, 126.78, 125.35, 116.42, 51.42, 48.94, 45.56, 42.42, 34.20, 16.86. FT-IR (cm−1): 2927.24, 2848.43, 1735.50, 1650.53, 1635.68, 1411.26, 1318.00 1211.36, 1069.26, 759.41, 725.13. GC-MS (EI): tR = 18.536 min, MW = 380.8, m/z[M+] = 380.1.
2-[(2E)-3-(4-Fluorophenyl)prop-2-enoyl]-6-methyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 26; light yellow crystals (0.12 g, 70.5%); m.p. 175–179 °C; Rf = 0,25 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.71 (d, J = 15.4 Hz, 1H), 7.56–7.47 (m, 2H), 7.39–7.25 (m, 2H), 7.25 (dd, J = 7.6, 3.8 Hz, 1H), 7.16 (d, J = 7.2 Hz, 1H), 7.12–7.00 (m, 2H), 6.74 (d, J = 15.4 Hz, 1H), 5.30–5.21 (m, 1H), 5.06 (d, J = 13.3 Hz, 1H), 4.80 (d, J = 9.9 Hz, 1H), 4.56 (d, J = 17.3 Hz, 1H), 4.24 (d, J = 17.4 Hz, 1H), 3.13 (dd, J = 16.0, 5.7 Hz, 1H), 3.06 (s, 1H), 2.62 (dd, J = 16.1, 2.2 Hz, 1H), 1.83 (s, 1H), 1.30–1.17 (m, 2H), 1.11 (d, J = 6.9 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 163.84, 163.77(JC-F = 250 Hz), 143.05, 130.01, 129.81 (JC-F = 8.08 Hz), 127.80, 127.80, 126.88, 125.45, 116.13, 115.92, 115.66, 115.16, 51.54, 45.67, 42.51, 34.32, 29.65, 16.95. FT-IR (cm−1): 3749.75, 2904.07, 1746.49, 1634.90, 1598.93, 1442.50, 1408.74, 1316.60, 1177.97, 1014.94, 828.27. GC-MS (EI): tR = 17.237 min, MW = 364.4, m/z[M+] = 362.2.
2-[(2E)-3-(4-Methoxy-phenyl)prop-2-enoyl]-6-methyl-1,2,3,6,7,11b-hexahydro-pyrido[2,1-a]isoquinolin-4-one 27; yellow crystals (0.14 g, 75.6%), m.p. 157–162 °C (dec.);Rf = 0.29 (diethyl ether); 1H NMR (400 MHz, CDCl3) δ 7.71 (d, J = 15.3 Hz, 1H), 7.52–7.43 (m, 2H), 7.35–7.20 (m, 3H), 7.14 (d, J = 6.9 Hz, 1H), 6.93–6.83 (m, 2H), 6.68 (d, J = 15.2 Hz, 1H), 5.24 (t, J = 7.5 Hz, 1H), 5.05 (s, 1H), 4.80 (s, 1H), 4.57 (d, J = 17.3 Hz, 1H), 4.22 (d, J = 17.4 Hz, 1H), 3.81 (s, 3H), 3.11 (dd, J = 16.1, 5.6 Hz, 1H), 3.03 (s, 1H), 2.60 (dd, J = 16.0, 2.2 Hz, 1H), 1.09 (d, J = 7.0 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 165.51, 161.18, 143.97, 132.46, 131.60, 29.93, 129.57, 127.69, 127.42, 126.80, 125.43, 114.26, 113.24, 55.29, 51.52, 49.00, 45.55, 42.39, 34.26, 16.88. FT-IR (cm−1): 3744.25, 2926.07, 2920.31, 1739.25, 1642.35, 1597.98, 1515.36, 1411.74, 1232.09, 1172.57, 1061.37, 826.26, 710.07, 769.16. GC-MS (EI): tR =19.040 min, MW = 376.4, m/z[M+] = 376.0.
2-[(2E)-3-(3-Hydroxyphenyl)prop-2-enoyl]-6-methyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 28; yellow crystals (0.16 g, 94.1%); m.p. 134–139 °C; Rf = 0.18 (diethyl ether); 1H NMR (400 MHz, CDCl3) δ 7.82–7.67 (m, 1H), 7.43–7.13 (m, 5H), 7.13–7.00 (m, 2H), 6.99–6.87 (m, 1H), 6.87–6.72 (m, 1H), 5.23 (d, J = 8.1 Hz, 1H), 5.03 (d, J = 13.2 Hz, 1H), 4.80 (s, 1H), 4.63–4.52 (m, 1H), 4.26 (dd, J = 17.5, 9.9 Hz, 1H), 3.17–2.99 (m, 2H), 2.66–2.57 (m, 1H), 1.10 (d, J = 6.8 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 165.66, 164.26, 157.10, 144.94, 135.85, 131.25, 130.07, 129.99, 127.81, 126.94, 125.51, 120.09, 117.80, 116.76, 115.55, 114.68, 51.57, 48.85, 45.77, 42.79, 34.22, 16.84. FT-IR (cm−1): 3200.24, 3189.30, 2932., 29.24, 1591.04, 1435.40, 1290.00, 1227.63, 1065.04, 970.86, 785.88, 755.95. GC-MS (EI): tR = 18.655 min, MW = 362.4, m/z[M+] = 362.2.
2-[(2E)-3-(3-Nitrophenyl)prop-2-enoyl]- 6-methyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 29; pale yellow solid (0.16 g, 87.1%); m.p. 160–164 °C (dec.); Rf = 0.35 (DCM-ethyl acetate, 1:1.5); 1H NMR (400 MHz, CDCl3) δ 8.40 (s, 1H), 8.23 (dd, J = 8.2, 2.3 Hz, 1H), 7.86–7.75 (m, 2H), 7.59 (t, J = 8.0 Hz, 1H), 7.39–7.25 (m, 3H), 7.18 (d, J = 7.3 Hz, 1H), 7.02–6.93 (m, 1H), 5.27 (dd, J = 9.7, 3.8 Hz, 1H), 5.07 (d, J = 13.5 Hz, 1H), 4.83 (d, J = 10.1 Hz, 1H), 4.59 (d, J = 16.9 Hz, 1H), 4.29 (d, J = 17.3 Hz, 1H), 3.13 (td, J = 19.5, 17.9, 8.7 Hz, 2H), 2.64 (d, J = 16.1 Hz, 1H), 1.13 (d, J = 7.1 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 164.36, 148.69, 141.49, 136.49, 133.65, 131.46, 129.99, 127.85, 126.94, 125.45, 124.36, 122.21, 119.09, 51.49, 49.09, 45.80, 42.61, 34.30, 16.96. FT-IR (cm−1): 1661.01, 1653.31, 1605.23, 1527.47, 1413.06, 1350.52, 1223.03, 975.04, 773.67, 737.99. GC-MS (EI): tR = 20.177 min, MW = 391.4, m/z[M+] = 391.1.
2-[(2E)-3-(2-Chlorophenyl)prop-2-enoyl]-6-methyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 30; yellow crystals (0.36 g, 51.0%); Rf = 0.32 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 8.11 (d, J = 15.2 Hz, 1H), 7.65–7.56 (m, 1H), 7.48–7.38 (m, 1H), 7.38–7.23 (m, 4H), 7.17 (s, 2H), 6.82 (d, J = 15.3 Hz, 1H), 5.25 (s, 1H), 5.08 (d, J = 7.6 Hz, 1H), 4.82 (s, 1H), 4.55 (d, J = 17.0 Hz, 1H), 4.24 (d, J = 17.2 Hz, 1H), 3.13 (dd, J = 16.1, 5.6 Hz, 1H), 3.06 (s, 1H), 2.66–2.58 (m, 1H), 1.12 (d, J = 7.0 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 164.90, 163.84, 140.25, 134.88, 133.13, 132.37, 131.48, 130.80, 130.24, 130.03, 127.77, 127.73, 127.05, 126.92, 125.46, 118.92, 51.55, 49.14, 45.72, 42.55, 34.33, 16.94. FT-IR (cm−1): 2919.38, 1642.39, 1412.65, 1318.97, 1205.08, 1037.32, 972.58, 756.64. GC-MS (EI): tR = 18.193 min, MW = 380.8, m/z[M+] = 380.1.
2-[(2E)-3-(2-Bromo-4-fluorophenyl)prop-2-enoyl]-6-methyl-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 31; colorless crystals (0.16 g, 75.0%); m.p. 182–186 °C; Rf = 0.23 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.74 (d, J = 6.4 Hz, 1H), 7.64 (d, J = 14.6 Hz, 1H), 7.44 (t, J = 6.3 Hz, 1H), 7.36–7.28 (m, 1H), 7.28 (s, 1H), 7.26 (d, J = 3.3 Hz, 1H), 7.15 (dt, J = 16.5, 10.0 Hz, 2H), 6.77 (d, J = 15.2 Hz, 1H), 5.28–5.20 (m, 1H), 5.05 (d, J = 13.1 Hz, 1H), 4.80 (d, J = 10.9 Hz, 1H), 4.57 (d, J = 16.5 Hz, 1H), 4.26 (d, J = 16.3 Hz, 1H), 3.11 (dt, J = 19.8, 9.7 Hz, 2H), 2.62 (d, J = 15.9 Hz, 1H), 1.11 (d, J = 6.8 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 163.67, 161.63 (JC-F = 202Hz), 161.14, 141.58, 132.74, 132.48, 131.49, 130.01, 128.71, 127.81, 126.89, 125.44, 117.02, 116.94 (JC-F = 22.22 Hz, 109.97, 109.81 (JC-F = 22,22 Hz), 51.50, 49.07, 45.71, 42.53, 34.30, 16.95. FT-IR (cm−1): 3748.28, 2972.49, 2927, 1667.50, 1646.87, 1603.11, 1449.33, 1411.68, 1248.82, 1002.85, 819.33, 758.47. GC-MS (EI): tR = 19.047 min, MW = 443.3, m/z[M-Br81+] = 444.1.
2-[(2E)-3-(4-Isopropyl-phenyl)-acryloyl]-6-methyl-1,2,3,6,7,11b-hexahydro-pyrazino[2,1-a]isoquinolin-4-one 32; white powder (0.16 g, 81.2%) m.p. 120–124 °C; Rf = 0.36 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.73 (d, J = 15.4 Hz, 1H), 7.50–7.37 (m, 2H), 7.35–7.24 (m, 1H), 7.23 (dd, J = 10.6, 4.2 Hz, 4H), 7.14 (d, J = 7.1 Hz, 1H), 6.79 (d, J = 15.4 Hz, 1H), 5.24 (q, J = 4.6 Hz, 1H), 5.06 (d, J = 13.3 Hz, 1H), 4.80 (s, 1H), 4.58 (d, J = 17.2 Hz, 1H), 4.23 (d, J = 17.3 Hz, 1H), 3.67 (q, J = 7.0 Hz, 1H), 3.11 (dd, J = 16.0, 5.6 Hz, 1H), 3.03 (s, 1H), 2.90 (p, J = 6.9 Hz, 1H), 2.64–2.53 (m, 1H), 1.27–1.14 (m, 7H), 1.09 (dd, J = 7.0, 2.0 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 165.47, 164.07, 151.36, 144.37, 132.39, 131.63, 129.97, 128.09, 127.74, 126.97, 126.85, 126.70, 126.34, 125.47, 51.56, 49.02, 45.63, 42.48, 34.28, 34.04, 23.70, 16.91. GC-MS (EI): tR = 19.105 min, MW = 388.2, m/z[M+] = 388.2.
2-[(2E)-3-(3-Benzo[1,3]dioxol-5-yl-acryloyl)-6-methyl-1,2,3,6,7,11b-hexahydro-pyrazino[2,1-a]isoquinolin-4-one 33; white powder (0.14 g, 76.0%); m.p. 75–79 °C; Rf = 0.25 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.56 (d, J = 15.3 Hz, 1H), 7.26 (t, J = 6.1 Hz, 4H), 7.15 (d, J = 7.1 Hz, 1H), 7.05–6.93 (m, 1H), 6.79 (dd, J = 9.6, 7.9 Hz, 1H), 6.61 (d, J = 15.4 Hz, 1H), 6.01–5.94 (m, 2H), 5.25 (t, J = 6.3 Hz, 1H), 4.22 (d, J = 17.3 Hz, 2H), 3.89 (d, J = 5.2 Hz, 1H), 3.34 (s, 1H), 3.04 (s, 1H), 2.61 (dd, J = 16.2, 2.2 Hz, 1H), 2.02 (s, 1H), 1.41 (s, 1H), 1.10 (d, J = 6.9 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 165.39, 164.10, 149.45, 148.32, 144.09, 136.92, 134.78, 129.17, 128.35, 126.87, 125.48, 124.26, 123.77, 113.75, 108.57, 106.43, 101.38, 51.57, 49.07, 45.63, 42.45, 34.32, 16.94. FT-IR (cm−1): 3748.03, 2920.18, 2850.43, 1734.83, 1595.54, 1490.12, 1446.75, 14.30.13, 1415.22, 1125.91, 1033.51, 806.93. GC-MS (EI): tR = 20.043 min, MW = 390.4, m/z[M+] = 390.2.
6-Ethyl-2-[(2E)-3-phenylprop-2-enoyl]-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one 34; yellow crystals (0.14 g, 85.0%); Rf = 0.38 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.76 (d, J = 15.4 Hz, 1H), 7.57–7.49 (m, 2H), 7.43–7.33 (m, 3H), 7.31 (s, 2H), 7.30–7.21 (m, 2H), 7.14 (d, J = 6.8 Hz, 1H), 6.83 (d, J = 15.4 Hz, 1H), 5.12 (d, J = 12.7 Hz, 1H), 5.03 (d, J = 7.2 Hz, 1H), 4.76 (s, 1H), 4.62 (d, J = 17.2 Hz, 1H), 4.25 (d, J = 17.2 Hz, 1H), 4.09 (qd, J = 7.2, 0.8 Hz, 1H), 3.13–2.96 (m, 2H), 2.71 (d, J = 16.2 Hz, 1H), 1.49–1.30 (m, 1H), 1.23 (td, J = 7.1, 0.8 Hz, 1H), 0.94–0.82 (m, 3H). 13C NMR (101 MHz, CDCl3) δ 171.09, 165.24, 144.36, 134.74, 130.10, 129.92, 128.88, 127.96, 127.69, 126.81, 125.50, 115.85, 60.34, 32.26, 23.73, 20.99, 14.15, 10.71. FT-IT (cm−1): 2961., 2925.15, 1643.23, 1412.92, 13.26,84, 1202.74, 1068.80, 976.12, 761.14. GC-MS (EI): tR = 17.559 min, MW = 360.4, m/z[M+] = 360.2.
2-[(2E)-3-(4-Chloro-phenyl)-acryloyl]-6-ethyl-1,2,3,6,7,11b-hexahydro-pyrazino[2,1-a]isoquinolin-4-one 35; yellow crystals (0.13 g, 77.0%); m.p. 116–120 °C; Rf = 0.43 (n-hexane –ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.69 (d, J = 15.4 Hz, 1H), 7.52–7.43 (m, 2H), 7.38–7.21 (m, 5H), 7.14 (d, J = 7.2 Hz, 1H), 6.81 (d, J = 15.2 Hz, 1H), 5.11–4.99 (m, 2H), 5.02 (q, J = 7.1 Hz, 1H), 4.78–4.71 (m, 1H), 4.61 (d, J = 17.1 Hz, 1H), 4.24 (d, J = 17.1 Hz, 1H), 3.12–2.93 (m, 2H), 2.70 (d, J = 16.2 Hz, 1H), 1.45–1.34 (m, 2H), 0.87 (s, 1H), 0.84 (d, J = 7.4 Hz, 2H). 13C NMR (101 MHz, CDCl3) δ 164.85, 164.13, 142.78, 135.84, 133.15, 132.50, 131.57, 129.84, 129.07, 129.05, 127.61, 126.73, 125.39, 116.39, 51.78, 48.89, 48.15, 45.71, 32.16, 23.63, 10.64. FT-IT (cm−1): 3904., 3727, 2915, 1641.02, 1606, 1404.49, 1218, 1013.11, 821.18, 752.02. GC-MS (EI): tR = 18.919 min, MW = 394.8, m/z[M+] = 394.2.
2-[(2E)-3-(4-Nitro-phenyl)-acryloyl]-6-ethyl-1,2,3,6,7,11b-hexahydro-pyrazino[2,1-a]isoquinolin-4-one  36; colorless crystals (0.14 g, 77.0%); m.p. 195–198 °C; Rf = 0.33 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 8.26–8.14 (m, 9H), 7.76 (d, J = 15.4 Hz, 4H), 7.71–7.60 (m, 9H), 7.30 (d, J = 7.3 Hz, 6H), 7.26–7.11 (m, 14H), 6.97 (d, J = 15.4 Hz, 4H), 5.06 (s, 2H), 5.04–4.97 (m, 4H), 4.83 (s, 1H), 4.75 (d, J = 9.7 Hz, 3H), 4.61 (d, J = 17.0 Hz, 3H), 4.27 (d, J = 17.0 Hz, 4H), 3.12–2.95 (m, 8H), 2.71 (d, J = 16.1 Hz, 5H), 1.45 (dd, J = 14.2, 7.1 Hz, 4H), 1.40 (s, 3H), 1.22 (s, 3H), 0.85 (t, J = 7.4 Hz, 15H). 13C NMR (101 MHz, CDCl3) δ 164.19, 163.93, 148.31, 141.33, 140.86, 132.57, 131.49, 129.93, 128.52, 127.75, 126.82, 125.40, 124.13, 124.02, 120.25, 51.79, 48.96, 48.30, 45.90, 32.20, 23.69, 10.69. FT-IT (cm−1): 29,424, 2862, 2170, 2011.81, 1634.73, 1613.56, 1514.06, 1407.55, 1341.60, 1288.73, 1237.90, 1065.93, 840.58, 764.12, 743.80. GC-MS (EI): tR = min, MW = 405.4, m/z[M+] = 405.1.
2-[(2E)-3-(4-Fluoro-phenyl)-acryloyl]- 6-ethyl-1,2,3,6,7,11b-hexahydro-pyrazino[2,1-a]isoquinolin-4-one  37; colorless crystals (0.11 g, 65.2%), Rf = 0.39 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.72 (d, J = 15.4 Hz, 1H), 7.57–7.48 (m, 2H), 7.35–7.19 (m, 3H), 7.18–6.99 (m, 4H), 6.75 (d, J = 15.4 Hz, 1H), 5.07 (dd, J = 33.2, 10.3 Hz, 2H), 4.76 (d, J = 10.2 Hz, 1H), 4.61 (d, J = 17.2 Hz, 1H), 4.25 (d, J = 17.3 Hz, 1H), 3.08 (dd, J = 16.1, 5.6 Hz, 1H), 3.04–2.94 (m, 1H), 2.72 (d, J = 16.2 Hz, 1H), 1.41 (s, 1H), 1.23 (s, 1H), 0.86 (t, J = 7.4 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 166.25, 164.20, 163.79(JC-F = 250Hz), 143.13, 132.65, 131.64, 131.00, 129.84 (JC-F = 8.08 Hz), 127.73, 126.84, 125.51, 116.45 (JC-F = 22 Hz), 115.56 (JC-F = 3.3 Hz), 94.99. FT-IT (cm−1): 3748.42, 2927.68, 2861, 1643.24, 1599.62, 1414.70, 1325.46, 1224.84, 1158.78, 827.04, 758.09. GC-MS (EI): tR = 17.447 min, MW = 378.4, m/z[M+] = 378.2.
2-[(2E)-3-(4-Methoxy-phenyl)-acryloyl]-6-ethyl-1,2,3,6,7,11b-hexahydro-pyrazino[2,1-a]isoquinolin-4-one 38; yellow crystals (0.04 g, 75.2%); m.p. 185–189 °C; Rf = 0.21 (n-hexane-ethyl acetate); 1H NMR (400 MHz, CDCl3) δ 7.72 (d, J = 15.3 Hz, 1H), 7.52–7.44 (m, 2H), 7.32 (s, 1H), 7.23 (s, 1H), 7.14 (d, J = 7.2 Hz, 1H), 6.93–6.83 (m, 2H), 6.73–6.65 (m, 1H), 5.12 (d, J = 13.7 Hz, 1H), 5.03 (q, J = 7.0 Hz, 1H), 4.74 (d, J = 9.9 Hz, 2H), 4.62 (d, J = 17.1 Hz, 1H), 4.23 (d, J = 17.4 Hz, 1H), 3.82 (s, 3H), 3.07 (dd, J = 16.2, 5.6 Hz, 1H), 2.96 (s, 1H), 2.70 (d, J = 16.2 Hz, 1H), 1.48–1.30 (m, 1H), 0.96–0.81 (m, 4H). 13C NMR (101 MHz, CDCl3) δ 165.56, 164.44, 161.21, 144.11, 132.55, 131.70, 129.89, 129.63, 127.63, 127.40, 126.78, 125.52, 114.28, 113.15, 55.33, 51.90, 48.96, 48.18, 45.69, 32.24, 23.67, 10.71. FT-IT (cm−1): 2967., 2929.95, 1637.42, 1597.15, 1413.28, 1413.25, 1253.52, 1237.26, 1173.90, 1029.37, 810.68. GC-MS (EI): tR = 19.373 min, MW = 390.4, m/z[M+] = 390.2.
2-[(2E)-3-(3,4-Dimethoxy-phenyl)-acryloyl]-6-ethyl-1,2,3,6,7,11b-hexahydro-pyrazino[2,1-a]isoquinolin-4-one  39; yellow solid (0.03 g, 88.8%); Rf = 0.31 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.72 (d, J = 15.2 Hz, 1H), 7.35–7.20 (m, 2H), 7.13 (t, J = 9.4 Hz, 2H), 7.05 (d, J = 1.7 Hz, 1H), 6.87 (d, J = 8.3 Hz, 1H), 6.68 (d, J = 15.3 Hz, 1H), 5.15 (d, J = 13.1 Hz, 1H), 5.04 (q, J = 7.2 Hz, 1H), 4.75 (s, 1H), 4.66 (d, J = 17.2 Hz, 1H), 4.26 (d, J = 17.4 Hz, 1H), 3.92 (d, J = 9.2 Hz, 6H), 3.59–3.42 (m, 1H), 3.09 (dd, J = 16.1, 5.5 Hz, 1H), 2.98 (s, 1H), 2.72 (d, J = 16.2 Hz, 1H), 2.03 (s, 1H), 1.48–1.29 (m, 1H), 0.88 (q, J = 7.6 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 165.45, 163.95, 159.19, 152.66, 150.99, 149.19, 144.43, 132.62, 130.01, 129.98, 129.28, 129.12, 127.76, 127.69, 126.83, 125.55, 122.75, 113.27, 111.02, 55.97, 51.97, 49,10, 48.17, 45.71, 32.29, 23.63, 10.75. GC-MS (EI): tR = 20.649 min, MW = 420.5, m/z[M+] = 420.2.
2-[(2E)-3-(2,4-Dimethoxy-phenyl)-acryloyl]-6-ethyl-1,2,3,6,7,11b-hexahydro-pyrazino[2,1-a]isoquinolin-4-one  40; yellowish solid (0.03 g, 85.0%); Rf = 0.41 (n-hexane-ethyl acetate, 1:1); 1H NMR (400 MHz, CDCl3) δ 7.92 (d, J = 15.4 Hz, 1H), 7.42 (d, J = 8.5 Hz, 1H), 7.35–7.23 (m, 1H), 7.27–7.19 (m, 1H), 7.14 (d, J = 7.4 Hz, 1H), 6.89 (d, J = 15.4 Hz, 1H), 6.54–6.43 (m, 2H), 5.15 (s, 1H), 5.04 (d, J = 7.2 Hz, 1H), 4.75 (d, J = 9.9 Hz, 1H), 4.63 (d, J = 17.7 Hz, 1H), 4.22 (d, J = 17.8 Hz, 1H), 3.91–3.68 (m, 6H), 3.09 (dd, J = 16.2, 5.6 Hz, 1H), 2.95 (s, 1H), 2.71 (d, J = 16.2 Hz, 1H), 1.48–1.31 (m, 1H), 0.95–0.70 (m, 4H). 13C NMR (101 MHz, CDCl3) δ 166.40, 164.65, 162.45, 159.90, 139.98, 131.32, 129.87, 127.64, 126.78, 125.64, 124.63, 120.08, 116.95, 114.11, 105.08, 98.53, 55.52, 55.46, 52.17, 48.25, 45.98, 32.31, 29.68, 23.71, 10.76. GC-MS (EI): tR = 21.055 min, MW = 420.5, m/z[M+] = 420.2.

5. Conclusions

Two PZQ analogues and seventeen molecular hybrids were successfully synthesized in a three-step procedure starting from the corresponding two 2-amino-1-phenylalkanes as precursors. The identity of the synthesized compounds was fully established by NMR spectroscopy, FTIR, and mass spectrometry, and their biological activity against P. falciparum and S. mansoni was evaluated. While none of the compounds were active against P. falciparum, the results identified four molecular hybrids with good activity against S. manson. Based on its activity, one particular molecular hybrid can be considered as a front runner, the optimization of which may generate a lead compound in the fight against schistosomiasis. An ortho substitution by a bromine, a chlorine atom substitution on the para position, and a substitution on the para position of cinnamic acid aromatic ring by a nitro group as well as by an isopropyl group, increases the activity of the molecule.
The strategy of molecular hybridization employed in this project, which involves coupling cinnamic acids with the PZQ tetrahydroisoquinoline scaffold, showed promising results in the development of novel compounds with potent activity.
This work also confirmed that substitution at the C6 position of the tetrahydroisoquinoline aromatic ring affects the activity of the molecular hybrid, which increases with the increase in the number of carbon atoms of the substituent, including the substitution on the para position on the cinnamic acid aromatic ring.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules28135184/s1, In-vitro anti-plasmodium activity, NMR spectra, HPLC-MS and IR spectra of compounds described in this study. Table S1. In vitro antiplasmodial activity evaluation of molecular hybrids and praziquantel analogues.

Author Contributions

Conceptualization, W.M.; methodology, W.M.; validation; formal analysis, C.H., F.M.K. and W.M.; investigation, J.K., F.M.K. and W.M.; resources, W.M.; data curation, J.K., F.M.K. and W.M.; writing—original draft preparation, F.M.K. and W.M.; writing—review and editing, F.M.K., J.K. and W.M.; supervision, W.M.; project administration, W.M. All authors have read and agreed to the published version of the manuscript.

Funding

This research received financial assistance from The Directorate of Research and Innovation of Walter Sisulu University. We thank Creppat Laboratories for financial assistance to F.M.K. J.K. is grateful to the Swiss National Science Foundation for financial support (No. 320030_175585/1).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data is shared in the paper and Supplementary Materials.

Acknowledgments

The logistical support received from the Drug Discovery and Development Centre (H3D) at the University of Cape Town is gratefully acknowledged.

Conflicts of Interest

The authors declare no conflict of interest.

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Molecules 28 05184 sch001 550
Scheme 1. Merck synthesis of PZQ.
Scheme 1. Merck synthesis of PZQ.
Molecules 28 05184 sch001
Molecules 28 05184 sch002 550
Scheme 2. Four-component Ugi synthesis of PZQ derivatives.
Scheme 2. Four-component Ugi synthesis of PZQ derivatives.
Molecules 28 05184 sch002
Molecules 28 05184 sch003 550
Scheme 3. Synthesis of praziquanamines 1.
Scheme 3. Synthesis of praziquanamines 1.
Molecules 28 05184 sch003
Molecules 28 05184 sch004 550
Scheme 4. Synthesis amphetamine and 1-phenylbutan-2-amine.
Scheme 4. Synthesis amphetamine and 1-phenylbutan-2-amine.
Molecules 28 05184 sch004
Molecules 28 05184 sch005 550
Scheme 5. Pictet-Spengler cyclisation of the iminium intermediate.
Scheme 5. Pictet-Spengler cyclisation of the iminium intermediate.
Molecules 28 05184 sch005
Molecules 28 05184 sch006 550
Scheme 6. Synthesis of praziquantel analogues and molecular hybrids.
Scheme 6. Synthesis of praziquantel analogues and molecular hybrids.
Molecules 28 05184 sch006
Table
Table 1. Synthesis of praziquantel analogues and molecular hybrids.
Table 1. Synthesis of praziquantel analogues and molecular hybrids.
Molecules 28 05184 i001
CompoundRR1R2R3Yield (%)
22Me---68.3
23Et---64.2
24MeHHH73.4
25MeHHCl93.6
26MeHHF70.5
27MeHHOme75.6
28MeHOHH94.1
29MeHNO2H87.1
30MeClHH51.0
31MeBrHF75.0
32MeHHiPr81.2
33MeH-O-CH2-O-76.0
34EtHHH85.0
35EtHHCl77.0
36EtHHNO277.0
37EtHHF65.2
38EtHHOme75.2
39EtHOmeOme88.8
40EtOmeHOme85.0
Table
Table 2. Antischistosomal worm killing activity of praziquantel analogues and molecular hybrids.
Table 2. Antischistosomal worm killing activity of praziquantel analogues and molecular hybrids.
NTSNTSS. mansoni AdultS. mansoni AdultS. mansoni Adult
Compound IDEffect in % (Dead, 72 h) and SD
Test Conc. 50 µM		Effect in % (Dead, 72 h) and SD
Test Conc. 10 µM		Effect in % (Dead, 72 h) and SD
Test Conc. 50 µM		Effect in % (Dead, 72 h) and SD
Test Conc. 10 µM		Effect in % (Dead, 72 h) and SD
Test Conc. 1 µM		IC50
Schisto Adult
(µM) (72 h)
2243.3 (3.3)20.8 (4.2)---
2375 (5)37.5 (4.2)---
2446.7 (6.7 < 9)43.8 (2.1)---
2566.7 (3.3)62.5 (0)---
2673.3 (6.7)18.8 (2.1)---
2755 (1.7)35.4 (2.1)---
2861.7 (1.7)31.3 (2.1)---
2968.3 (1.7)29.2 (0)---
3063.3 (3.3)25 (0)---
31100 (0)35.4 (2.1)81.5 (3.7)34.5 (0) 12.7
32100 (0)50 (0)100 (0)78.2 (0)38.9 (5.6)1.9
3366.7 (3.3)35.4 (2.1)---
3488.3 (1.7)35.4 (2.1)33.3 (0)--
35100 (0)64.6 (2.1)96.3 (0)30.9 (0) 13.8
3695 (1.7)33.3 (4.2)48.2 (3.7)--
3773.3 (3.3)31.3 (2.1)---
3845 (1.7)37.5 (0)---
3945 (3.3)27.1 (2.1)---
4085 (1.7)29.2 (4.2)77.8 (0)34.5 (0)-14.7
PZQ100 (0)-100 (0)100 (0)91.7 (6.5)
NTS: newly transformed schistosomula.
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MDPI and ACS Style

Kasago, F.M.; Häberli, C.; Keiser, J.; Masamba, W. Design, Synthesis and Evaluation of Praziquantel Analogues and New Molecular Hybrids as Potential Antimalarial and Anti-Schistosomal Agents. Molecules 2023, 28, 5184. https://doi.org/10.3390/molecules28135184

AMA Style

Kasago FM, Häberli C, Keiser J, Masamba W. Design, Synthesis and Evaluation of Praziquantel Analogues and New Molecular Hybrids as Potential Antimalarial and Anti-Schistosomal Agents. Molecules. 2023; 28(13):5184. https://doi.org/10.3390/molecules28135184

Chicago/Turabian Style

Kasago, Freddy Mugisho, Cécile Häberli, Jennifer Keiser, and Wayiza Masamba. 2023. "Design, Synthesis and Evaluation of Praziquantel Analogues and New Molecular Hybrids as Potential Antimalarial and Anti-Schistosomal Agents" Molecules 28, no. 13: 5184. https://doi.org/10.3390/molecules28135184

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