Prevalence and Characteristics of Invasive Staphylococcus argenteus among Patients with Bacteremia in Hong Kong
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. MALDI-TOF MS Identification
2.3. Phenotypic Antimicrobial Resistance Testing
2.4. Multiplex Real-Time PCR
2.5. Whole-Genome Sequencing
2.6. Molecular Epidemiology Analysis
2.7. AMR and Virulence Factor Prediction
3. Results
3.1. Prevalence of Invasive S. argenteus in Blood Culture Specimens
3.2. MALDI-TOF MS Identification for S. argenteus Strains
3.3. Genotypes of Invasive S. argenteus in Hong Kong
3.4. AMR and Virulence Factors
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample No. | Date of Isolation | Gender | Age | Phenotypic and MALDI-TOF MS Identification | Genotypic Identification | ||||
---|---|---|---|---|---|---|---|---|---|
Gram Stain | Coagulase | MALDI-TOF MS (ID ≥ 2.0) | nuc/sau | mecA | coa | ||||
S7 | 29/01/2020 | F | 53 | GPC | P | S. argenteus/S. schweitzeri/S. aureus | N | N | P |
S28 | 09/03/2020 | M | 84 | GPC | P | S. argenteus/S. schweitzeri/S. aureus | N | N | P |
S78 | 13/07/2020 | M | 62 | GPC | P | S. argenteus/S. aureus | N | N | P |
S95 | 21/08/2020 | F | 43 | GPC | P | S. argenteus/S. schweitzeri/S. aureus | N | N | P |
S106 | 12/09/2020 | M | 84 | GPC | P | S. argenteus/S. schweitzeri/S. aureus | N | N | P |
S137 | 07/12/2020 | M | 74 | GPC | P | S. argenteus/S. schweitzeri/S. aureus | N | N | P |
S167 | 22/01/2021 | M | 69 | GPC | P | S. argenteus/S. aureus | N | N | P |
S169 | 23/01/2021 | F | 72 | GPC | P | S. argenteus/S. schweitzeri/S. aureus | N | N | P |
S177 | 03/02/2021 | M | 59 | GPC | P | S. argenteus/S. schweitzeri/S. aureus | N | N | P |
S178 | 05/02/2021 | M | 50 | GPC | P | S. argenteus/S. schweitzeri | N | N | P |
S229 | 22/06/2021 | M | 93 | GPC | P | S. argenteus/S. schweitzeri | N | N | P |
Strain | MLST | spa type | Kirby–Bauer Phenotypic Resistance Test | Antimicrobial-Resistant Genes | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Amoxicillin and Clavulanate | Cefoxitin | Ceftaroline | Clindamycin | Cotrimoxazole | Erythromycin | Fusidic Acid | D-Zone | Gentamycin | Levofloxacin | Minocycline | Penicillin | Rifampin | Vancomycin | fosB | tet-38 | tet-L | mepA | aph(3′)-IIIa | sat4 | ant(6)-Ia | blaI | blaR1 | blaZ | |||
S7 | ST2250 | t7960 | S | S | S | S | S | S | S | − | S | S | S | S | S | S | ||||||||||
S28 | ST2250 | New1 | S | S | S | R | S | R | S | + | S | S | S | S | S | S | ||||||||||
S78 | ST2854 | New2 | S | S | S | S | S | S | S | − | S | S | S | R | S | S | ||||||||||
S95 | ST2250 | t5078 | S | S | S | S | S | S | S | − | S | S | S | R | S | S | ||||||||||
S106 | ST1223 | New3 | S | S | S | S | S | S | S | − | S | S | S | S | S | S | ||||||||||
S137 | ST1223 | t5142 | S | S | S | S | S | S | S | − | S | S | S | S | S | S | ||||||||||
S167 | ST2854 | New4 | S | S | S | S | S | S | S | − | S | S | S | R | S | S | ||||||||||
S169 | ST2250 | t17928 | S | S | S | S | S | S | S | − | S | S | S | R | S | S | ||||||||||
S177 | ST1223 | New5 | S | S | S | S | S | S | S | − | S | S | S | S | S | S | ||||||||||
S178 | ST2250 | t5078 | S | S | S | S | S | S | S | − | S | S | S | R | S | S | ||||||||||
S229 | ST1223 | t12782 | S | S | S | S | S | S | S | − | S | S | S | R | S | S |
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Chen, J.H.K.; Leung, H.-Y.; Wong, C.M.C.; Yuen, K.-Y.; Cheng, V.C.C. Prevalence and Characteristics of Invasive Staphylococcus argenteus among Patients with Bacteremia in Hong Kong. Microorganisms 2023, 11, 2435. https://doi.org/10.3390/microorganisms11102435
Chen JHK, Leung H-Y, Wong CMC, Yuen K-Y, Cheng VCC. Prevalence and Characteristics of Invasive Staphylococcus argenteus among Patients with Bacteremia in Hong Kong. Microorganisms. 2023; 11(10):2435. https://doi.org/10.3390/microorganisms11102435
Chicago/Turabian StyleChen, Jonathan H. K., Hoi-Yi Leung, Charles M. C. Wong, Kwok-Yung Yuen, and Vincent C. C. Cheng. 2023. "Prevalence and Characteristics of Invasive Staphylococcus argenteus among Patients with Bacteremia in Hong Kong" Microorganisms 11, no. 10: 2435. https://doi.org/10.3390/microorganisms11102435