The Protective and Therapeutic Anti-Alzheimer Potential of Olea europaea L. cv. Picual: An In Silico and In Vivo Study
Abstract
:1. Introduction
2. Results
2.1. Chemical Dereplication of Olea europaea L. cv. Picual
2.2. Identified Compounds—Target Network
2.3. Target Correlation to Specific Genes with Alzheimer’s Disease
2.4. Olea europaea L. cv. Picual Compounds Target Alzheimer and Memory Disorders
2.5. Protein–Protein Interactions
2.6. Gene Enrichment Analysis
2.7. Functional Annotation Clustering of KEGG Pathways
2.8. Biological Pathways
2.9. Behavioral Assessment Using T-Maze
2.10. Biochemical Analysis
2.11. Protein Expression of Aβ Peptide and Tau
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Chemicals and Reagents
4.3. Plant Material Extraction
4.4. Metabolomic Analysis Procedure
4.5. Identified Compounds–Targets
4.6. Targets Correlation to Specific Genes with Alzheimer’s Disease
4.7. Protein–Protein Interaction (PPI)
4.8. Network Construction and Visualization
4.9. Gene Enrichment Analysis
4.10. Animals and Ethics
4.11. Experimental Design
- Group (1): Normal healthy rats served as negative control.
- Group (2): Alzheimer’s disease (AD)-induced rats received AlCl3 orally at a dose of 17 mg/kg body weight daily for 21 days as described before [62].
- Group (3): AD-induced/prophylactic rats received AlCl3 and OEP extract orally (100 mg/kg.b.w.) together from day 1 to 21 as a prophylactic approach [63].
- Group (4): AD-induced/treated rats received AlCl3 from day 1 to 21, followed by OEP extract treatment orally (100 mg/kg.b.w.) from day 22 to 42.
4.12. T-Maze Test
4.13. Biochemical Analysis
4.14. Western Blotting Analysis
4.15. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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No. | Metabolite Name | Original Source | MF | RT (min) | m/z |
---|---|---|---|---|---|
1 | Syringic acid | Olea europaea | C9H11O5 | 0.67 | 199.0606 |
2 | Oleuropein | Olea europaea | C25H32O13 | 3.02 | 541.1921 |
3 | Verbascoside | Olea europaea | C29H36O15 | 3.03 | 623.1980 |
4 | p-Coumaric acid | Olea europaea | C9H9O3 | 3.94 | 165.1660 |
5 | Demethyloleuropein | Olea europaea | C24H31O13 | 4.48 | 527.1765 |
6 | Hydroxytyrosol | Olea europaea | C8H11O3 | 5.19 | 155.0708 |
7 | Chlorogenic acid | Olea europaea | C16H19O9 | 5.60 | 355.1029 |
8 | 2,3-Dihydroxy-13(18)-oleanen-28-oic acid | Olea europaea | C30H48O4 | 5.96 | 473.36213 |
9 | Caffeic acid | Olea europaea | C9H9O4 | 6.19 | 181.0501 |
10 | 11-Octadecen-9-ynoic acid | Olea europaea | C18H30O2 | 6.73 | 277.2167 |
11 | Quercetin | Olea europaea | C15H11O7 | 9.56 | 303.0505 |
12 | Oleoside | Olea europaea | C18H27O11 | 10.30 | 419.1553 |
13 | Nuzhenide | Olea europaea | C30H41O17 | 10.56 | 673.2344 |
14 | Apigenin | Olea europaea | C15H11O5 | 10.58 | 271.0606 |
15 | Apigenin-7-O-β-D-rutinoside | Olea europaea | C27H31O14 | 12.14 | 579.1714 |
16 | Apigenin-3-O-β-D-glucopyranoside | Olea europaea | C21H21O11 | 13.33 | 449.1084 |
17 | Rutin | Olea europaea | C27H31O16 | 17.59 | 611.1612 |
18 | 3-Hydroxy-12-oleanen-28-oic acid | Olea europaea | C30H50O2 | 29.26 | 443.3880 |
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Bagalagel, A.A.; El-hawary, S.S.; Alaaeldin, R.; Elmaidomy, A.H.; Altemani, F.H.; Waggas, D.S.; Algehainy, N.A.; Saeedi, N.H.; Alsenani, F.; Mokhtar, F.A.; et al. The Protective and Therapeutic Anti-Alzheimer Potential of Olea europaea L. cv. Picual: An In Silico and In Vivo Study. Metabolites 2022, 12, 1178. https://doi.org/10.3390/metabo12121178
Bagalagel AA, El-hawary SS, Alaaeldin R, Elmaidomy AH, Altemani FH, Waggas DS, Algehainy NA, Saeedi NH, Alsenani F, Mokhtar FA, et al. The Protective and Therapeutic Anti-Alzheimer Potential of Olea europaea L. cv. Picual: An In Silico and In Vivo Study. Metabolites. 2022; 12(12):1178. https://doi.org/10.3390/metabo12121178
Chicago/Turabian StyleBagalagel, Alaa A., Seham S. El-hawary, Rania Alaaeldin, Abeer H. Elmaidomy, Faisal H. Altemani, Dania S. Waggas, Naseh A. Algehainy, Nizar H. Saeedi, Faisal Alsenani, Fatma A. Mokhtar, and et al. 2022. "The Protective and Therapeutic Anti-Alzheimer Potential of Olea europaea L. cv. Picual: An In Silico and In Vivo Study" Metabolites 12, no. 12: 1178. https://doi.org/10.3390/metabo12121178