Comparative Analysis of Transposable Elements in Genus Calliptamus Grasshoppers Revealed That Satellite DNA Contributes to Genome Size Variation
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection, Genome Size Estimation, and Next-Generation Sequencing
2.2. Quality Check, Random Sampling, and Pre-Processing of the Sequenced Data
2.3. Species Code Assignment, Reads Sub-Sampling, Concatenation and Comparative RepeatExplorer2 and TAREAN Clustering Analysis
2.4. Homology Searches, Comparative Satellitome Analysis, and Z-Score Values
2.5. Comparative Repeat Profiling of Satellite DNA
3. Results
3.1. Individual Clustering Analysis and Composition of Repeat among Three Genomes
3.2. Comparative Visualization of Repeat Content in Genus Calliptamus Species
3.2.1. Satellite DNA Characterization and Homology Searches
3.2.2. The Estimation of satDNA Abundance, Divergence, and Copy Number
3.2.3. Comparison of Satellitome Landscapes
3.3. Z-Score Abundance and Divergence and Repeat Profiling of Satellite DNAs/rDNAs
4. Discussion
4.1. Genome Sizes and Divergence Timescale of Calliptamus Grasshoppers
4.2. Repeatome Composition and Diversity among the Genomes
4.3. Satellitome Analysis and satDNAs Family Number Differences
4.4. Satellite DNA Familiies Monomer Size Variation and Double Peak Pattern
4.5. Z-Score Abundance and Divergence Values and TEs Profiling
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Calliptamus italicus | Calliptamus barbarus | Calliptamus abbreviatus | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Repeat name | Length (bp) | A+T% | R.Length (bp) | K2P Divergence | % Abundance | Copy Number | R.Length (bp) | K2P Divergence | % Abundance | Copy Number | R.Length (bp) | K2P Divergence | % Abundance | Copy Number |
CSat01 | 880 | 51 | 1,868,736 | 8.09 | 0.2377 | 2679288 | 2,524,598 | 12.26 | 0.3211 | 3566082 | 2,914,794 | 3.21 | 0.4716 | 5005862 |
CSat02 | 138 | 52 | 2,047,691 | 9.55 | 0.2605 | 18721455 | 1,923,378 | 4.11 | 0.2447 | 17324765 | 2,856,892 | 7.84 | 0.4622 | 31287328 |
CSat03 | 159 | 52 | 2,086,105 | 3.1 | 0.2654 | 16553632 | 689 | 7.8 | 0.000087 | 5386 | 216,925 | 9.46 | 0.0351 | 2061893 |
CSat04 | 181 | 59 | 589,404 | 4.79 | 0.0749 | 4108551 | 1,542,589 | 5.83 | 0.1962 | 10593841 | 37,444 | 15.15 | 0.0060 | 312649 |
CSat05 | 270 | 58 | 841,952 | 10.69 | 0.1071 | 3934393 | 623,587 | 11.31 | 0.0793 | 2870880 | 504,540 | 11.81 | 0.0816 | 2824135 |
CSat06 | 26 | 54 | 8891 | 18.61 | 0.0011 | 431451 | 917,670 | 4.44 | 0.1167 | 43872762 | 55,901 | 20.11 | 0.0090 | 3249376 |
CSat07 | 1544 | 51 | 126,042 | 6.2 | 0.0160 | 102996 | 38,987 | 7.91 | 0.0049 | 31387 | 207,484 | 3.69 | 0.0335 | 203091 |
CSat08 | 294 | 53 | 65,871 | 6.15 | 0.0083 | 282683 | 26,312 | 19.21 | 0.0033 | 111247 | 95,366 | 5.64 | 0.0154 | 490230 |
CSat09 | 130 | 61 | 2,457,238 | 11.92 | 0.3126 | 23848338 | 1,984,831 | 12.19 | 0.2525 | 18978504 | 1,865,417 | 12.15 | 0.3018 | 21686343 |
CSat10 | 1246 | 55 | 1,147,659 | 6.55 | 0.1460 | 1162114 | 1,280,293 | 6.43 | 0.1628 | 1277241 | 2,613,887 | 4.83 | 0.4229 | 3170461 |
CSat11 | 220 | 59 | 439,785 | 8.85 | 0.0559 | 2522156 | 513,195 | 7.75 | 0.0652 | 2899622 | 586,905 | 11.32 | 0.0949 | 4031799 |
CSat12 | 42 | 39 | 681 | 10.09 | 0.000086 | 20457 | 598,775 | 7.43 | 0.0761 | 17721324 | NA | NA | NA | NA |
CSat13 | 2150 | 49 | 261,807 | 15.46 | 0.0333 | 153637 | 279,754 | 15.42 | 0.0355 | 161740 | 271,878 | 13.69 | 0.0439 | 191112 |
CSat14 | 862 | 52 | 61,717 | 8.68 | 0.0078 | 90334 | 60,903 | 10.53 | 0.0077 | 87824 | 91,750 | 9.6 | 0.0148 | 160861 |
CSat15 | 1533 | 53 | 61,281 | 8.14 | 0.0077 | 50435 | 93,124 | 9.26 | 0.0118 | 75509 | 150,566 | 7.78 | 0.0243 | 148435 |
CSat16 | 225 | 58 | 156,325 | 5.51 | 0.0198 | 876597 | 11,758 | 6.42 | 0.0014 | 64958 | 90,354 | 9.05 | 0.0146 | 606902 |
CSat17 | 223 | 59 | 206,411 | 7.01 | 0.0262 | 1167837 | 5982 | 7.19 | 0.0007 | 33344 | 64,944 | 7.84 | 0.0105 | 440137 |
CSat18 | 185 | 60 | 70,952 | 9.64 | 0.0090 | 483890 | 81,286 | 9.32 | 0.0103 | 546167 | 78,435 | 9.49 | 0.0126 | 640754 |
CSat19 | 897 | 53 | 41,687 | 3.73 | 0.0053 | 58635 | 34,831 | 11.18 | 0.0044 | 48267 | 128,261 | 2.5 | 0.0207 | 216100 |
CSat20 | 245 | 60 | NA | NA | NA | NA | 103,174 | 13.21 | 0.0131 | 523462 | NA | NA | NA | NA |
Total | 1.5954 | 1.5957 | 2.0763 | |||||||||||
Mean | 8.566315789 | 9.262631579 | 9.175555556 | |||||||||||
SD | 3.828965933 | 3.767555709 | 4.495353447 |
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Majid, M.; Yuan, H. Comparative Analysis of Transposable Elements in Genus Calliptamus Grasshoppers Revealed That Satellite DNA Contributes to Genome Size Variation. Insects 2021, 12, 837. https://doi.org/10.3390/insects12090837
Majid M, Yuan H. Comparative Analysis of Transposable Elements in Genus Calliptamus Grasshoppers Revealed That Satellite DNA Contributes to Genome Size Variation. Insects. 2021; 12(9):837. https://doi.org/10.3390/insects12090837
Chicago/Turabian StyleMajid, Muhammad, and Huang Yuan. 2021. "Comparative Analysis of Transposable Elements in Genus Calliptamus Grasshoppers Revealed That Satellite DNA Contributes to Genome Size Variation" Insects 12, no. 9: 837. https://doi.org/10.3390/insects12090837