Global Transcriptomic Analysis of Placentas from Women with Gestational SARS-CoV-2 Infection during the Third Trimester of Pregnancy
Abstract
:1. Introduction
2. Results
2.1. Clinical Characteristics of SARS-CoV-2-Positive and Healthy Women
2.2. Results from qPCR for SARS-CoV-2 Detection in Term Placenta
2.3. Differential Gene Expression Analysis of SARS-CoV-2-Positive and Healthy Women
2.4. Differentially Expressed Genes in Symptomatic Women Compared with Healthy Women
2.5. Comparison of Pathway Enrichment Analysis between Symptomatic and Healthy Women
3. Discussion
4. Materials and Methods
4.1. Study Population
4.2. Sample Collection
4.3. RNA Extraction
4.4. qPCR for SARS-CoV-2 Detection
4.5. mRNA Library Preparation and Sequencing
4.6. Sequencing Data Processing and Bioinformatic Analysis
mRNA Data Analysis
4.7. Validation of RNAseq Data by qPCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Case | Maternal Age (Years) | Gestational Age (Weeks + Days) | Delivery Mode | Maternal Co-Morbidities | Long-Term Medication, If Any | COVID-19 Status | Time from SARS-CoV-2-Positive Result to Delivery (Days) | Severity of COVID-19 at the Time of Delivery | Placental Pathology | Gender | Newborn Apgar Score (1, 5, 10 min) | Neonate Symptoms/ Complications |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 32 | 40 + 3 | Vaginal | GDM a | Metformin | Active c | 0 | Asymptomatic | Normal | Female | 10-10-10 | Healthy |
4 | 41 | 41 + 2 | C-section | - | - | Active c | 1 | Asymptomatic | Normal | Male | 08-08-10 | Respiratory distress syndrome |
13 | 31 | 34 + 5 | C-section | GDM, Placenta accreta | - | Active c | 4 | Mild: cough | Placenta accreta | Male | 09-09-09 | Healthy |
14 | 35 | 39 + 4 | Vaginal | - | - | Active c | 0 | Mild: cough | Normal | Male | 09-10-10 | Healthy |
15 * | 32 | 37 + 5 | C-section | - | - | Past d | 110 | Asymptomatic | Normal | Female | 09-10-10 | Respiratory distress syndrome |
16 | 31 | 40 + 5 | Vaginal | - | - | Active c | 1 | Mild: cough; chills | Normal | Male | 08-09-10 | Healthy |
17 | 37 | 39 + 3 | C-section | PIH b, Polycystic kidney | - | Active c | 4 | Mild: cough; wheezing | Normal | Male | 09-10-10 | Healthy |
Gene Symbol | Gene Name | Fold Change | FDR |
---|---|---|---|
OR5AS1 | Olfactory Receptor Family 5 Subfamily AS Member 1 | 108.7 | 5.92 × 10−6 |
RNF212 | Ring Finger Protein 212 | 58.26 | 3.27 × 10−5 |
OR51M1 | Olfactory Receptor Family 51 Subfamily M Member 1 | 53.85 | 2.71 × 10−4 |
OR4K13 | Olfactory Receptor Family 4 Subfamily K Member 13 | 52.39 | 1.98 × 10−4 |
CLDN18 | Claudin 18 | 44.05 | 3.95 × 10−3 |
NTRK3 | Neurotrophic Receptor Tyrosine Kinase 3 | 42.44 | 2.21 × 10−5 |
CNNM1 | Cyclin And CBS Domain Divalent Metal Cation Transport Mediator 1 | 41.80 | 5.04 × 10−4 |
COL13A1 | Collagen Type XIII Alpha 1 Chain | 41.45 | 1.02 × 10−3 |
OR10G3 | Olfactory Receptor Family 10 Subfamily G Member 3 | 40.21 | 1.58 × 10−3 |
LRRC38 | Leucine-Rich Repeat Containing 38 | 39.64 | 1.27 × 10−5 |
CGB5 | Chorionic Gonadotropin Subunit Beta 5 | −53.67 | 3.78 × 10−3 |
EBI3 | Epstein–Barr Virus-Induced 3 | −49.80 | 7.93 × 10−7 |
CGB8 | Chorionic Gonadotropin Subunit Beta 8 | −38.49 | 5.67 × 10−3 |
TFPI2 | Tissue Factor Pathway Inhibitor 2 | −35.59 | 2.72 × 10−7 |
CRH | Corticotropin-Releasing Hormone | −34.22 | 1.51 × 10−5 |
GDF15 | Growth Differentiation Factor 15 | −33.29 | 5.33 × 10−7 |
KISS1 | KiSS-1 Metastasis Suppressor | −32.69 | 6.5 × 10−3 |
PSG9 | Pregnancy-Specific Beta-1-Glycoprotein 9 | −31.00 | 2.11 × 10−6 |
PSG1 | Pregnancy-Specific Beta-1-Glycoprotein 1 | −28.37 | 1.47 × 10−7 |
ALPP | Alkaline Phosphatase, Placental | −27.94 | 1.8 × 10−5 |
Description | Enrichment Score | p-Value | FDR |
---|---|---|---|
Oxidative phosphorylation | 24.48 | <0.001 | <0.001 |
Insulin secretion | 11.01 | <0.001 | <0.001 |
Cortisol synthesis and secretion | 6.44 | 0.002 | 0.028 |
Estrogen signaling pathway | 6.35 | 0.002 | 0.029 |
Antigen processing and presentation | 5.97 | 0.003 | 0.039 |
GnRH secretion | 4.81 | 0.008 | 0.109 |
Endocrine and other factor-regulated calcium reabsorption | 3.82 | 0.022 | 0.218 |
Oxytocin signaling pathway | 3.60 | 0.027 | 0.257 |
Fatty acid elongation | 3.30 | 0.037 | 0.320 |
GnRH signaling pathway | 3.24 | 0.039 | 0.320 |
Apelin signaling pathway | 3.01 | 0.049 | 0.364 |
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Tang, Y.; Boggavarapu, N.R.; Aronsson, A.; Gemzell-Danielsson, K.; Lalitkumar, P.G. Global Transcriptomic Analysis of Placentas from Women with Gestational SARS-CoV-2 Infection during the Third Trimester of Pregnancy. Int. J. Mol. Sci. 2024, 25, 1608. https://doi.org/10.3390/ijms25031608
Tang Y, Boggavarapu NR, Aronsson A, Gemzell-Danielsson K, Lalitkumar PG. Global Transcriptomic Analysis of Placentas from Women with Gestational SARS-CoV-2 Infection during the Third Trimester of Pregnancy. International Journal of Molecular Sciences. 2024; 25(3):1608. https://doi.org/10.3390/ijms25031608
Chicago/Turabian StyleTang, Yiqun, Nageswara Rao Boggavarapu, Annette Aronsson, Kristina Gemzell-Danielsson, and Parameswaran Grace Lalitkumar. 2024. "Global Transcriptomic Analysis of Placentas from Women with Gestational SARS-CoV-2 Infection during the Third Trimester of Pregnancy" International Journal of Molecular Sciences 25, no. 3: 1608. https://doi.org/10.3390/ijms25031608