Mitochondrial p38 Mitogen-Activated Protein Kinase: Insights into Its Regulation of and Role in LONP1-Deficient Nematodes
Abstract
:1. Introduction
2. Results
2.1. The MAPKmt Pathway Is Active in Lonp-1-Deficient Mutants
2.2. ATFS-1 Counteracts Activation of the MAPKmt Pathway upon Lonp-1 Inactivation
2.3. ATFS-1 Suppresses the Induction of cbp-3 in Lonp-1 Mutants
2.4. Induction of the MAPKmt Pathway in Lonp-1 Mutants Is Not MAD Pathway-Dependent
2.5. Loss of Lonp-1 Induces the ZIP-2 Innate Immune Response Effector
2.6. Disruption of the MAPKmt Pathway Influences Lonp-1 Mutants’ Lifespan and Stress Responses
3. Discussion
4. Materials and Methods
4.1. C. elegans Strains and Culture Conditions
4.2. RNA Interference
4.3. Microscopic Analysis
4.4. Growth Rate and Fecundity Assays
4.5. Lifespan Analysis
4.6. Stress Sensitivity Assays
4.7. RNA Extraction and Quantitative Reverse Transcription PCR (qRT-PCR)
4.8. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
MAPK | Mitogen-Activated Protein Kinase |
MAPKK | Mitogen-Activated Protein Kinase Kinase |
MAP3K | Mitogen-Activated Protein Kinase Kinase Kinase |
MAPKmt | Mitochondrial MAPK |
UPRmt | Mitochondrial Unfolded Protein Response |
UPRcyt | Cytoplasmic Unfolded Protein Response |
ATF | Activating Transcription Factor |
SARM | Sterile α and Armadillo Motif |
ASK1 | Apoptosis Signal-Regulating Kinase 1 |
MKK | Mitogen-Activated Protein Kinase Kinase |
NRF | Nuclear Factor-Erythroid 2-Related Factor |
FOXO | Forkhead Box O |
CBP | CREB-Binding Protein |
MAD | Mitochondrial-Associated Degradation |
bZIP | Basic Leucine Zipper |
RNAi | RNA Interference |
tBHP | Tert-Butyl Hydroperoxide |
hsp | Heat Shock Protein |
HSF | Heat Shock Factor |
HSR | Heat Shock Response |
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Taouktsi, E.; Kyriakou, E.; Voulgaraki, E.; Verganelakis, D.; Krokou, S.; Rigas, S.; Voutsinas, G.E.; Syntichaki, P. Mitochondrial p38 Mitogen-Activated Protein Kinase: Insights into Its Regulation of and Role in LONP1-Deficient Nematodes. Int. J. Mol. Sci. 2023, 24, 17209. https://doi.org/10.3390/ijms242417209
Taouktsi E, Kyriakou E, Voulgaraki E, Verganelakis D, Krokou S, Rigas S, Voutsinas GE, Syntichaki P. Mitochondrial p38 Mitogen-Activated Protein Kinase: Insights into Its Regulation of and Role in LONP1-Deficient Nematodes. International Journal of Molecular Sciences. 2023; 24(24):17209. https://doi.org/10.3390/ijms242417209
Chicago/Turabian StyleTaouktsi, Eirini, Eleni Kyriakou, Evangelia Voulgaraki, Dimitris Verganelakis, Stefania Krokou, Stamatis Rigas, Gerassimos E. Voutsinas, and Popi Syntichaki. 2023. "Mitochondrial p38 Mitogen-Activated Protein Kinase: Insights into Its Regulation of and Role in LONP1-Deficient Nematodes" International Journal of Molecular Sciences 24, no. 24: 17209. https://doi.org/10.3390/ijms242417209