Contribution of a WRKY Transcription Factor, ShWRKY81, to Powdery Mildew Resistance in Wild Tomato
Abstract
:1. Introduction
2. Results
2.1. WRKY18 and WRKY81 Were Differentially Expressed in Tomato following O. neolycopersici Infection
2.2. Characterization of ShWRKY81
2.3. Expression Patterns of ShWRKY81 in Response to Abiotic Stresses and Defense Signaling Hormones
2.4. Silencing of ShWRKY81 Resulted in a Loss of Host Resistance to On-Lz in LA1777
2.5. Silencing of ShWRKY81 Decreased Defense Responses following On-Lz Infection
2.6. Silencing of ShWRKY81 Reduced SA Accumulation and Led to Down-Regulated SA-Dependent PRs Gene Expression following On-Lz Infection
3. Discussion
4. Materials and Methods
4.1. Strains and Plant Growth
4.1.1. Powdery Mildew Production for Tomato Infection
4.1.2. Production of Bacterial Strains Used for Gene Silencing
4.1.3. Plant Material and Growth Conditions
4.2. Inoculation and Treatment
4.3. Cloning of ShWRKY81 and Bioinformatic Analyses
4.4. Subcellular Localization Analysis
4.5. Quantitative Real-Time PCR(qRT-PCR) Analysis
4.6. TRV2-Mediated ShWRKY81 Silencing
4.7. Histological and Phenotypic Observations
4.8. Phytohormone Determination
4.9. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, H.; Gong, W.; Wang, Y.; Ma, Q. Contribution of a WRKY Transcription Factor, ShWRKY81, to Powdery Mildew Resistance in Wild Tomato. Int. J. Mol. Sci. 2023, 24, 2583. https://doi.org/10.3390/ijms24032583
Wang H, Gong W, Wang Y, Ma Q. Contribution of a WRKY Transcription Factor, ShWRKY81, to Powdery Mildew Resistance in Wild Tomato. International Journal of Molecular Sciences. 2023; 24(3):2583. https://doi.org/10.3390/ijms24032583
Chicago/Turabian StyleWang, Han, Wenfeng Gong, Yang Wang, and Qing Ma. 2023. "Contribution of a WRKY Transcription Factor, ShWRKY81, to Powdery Mildew Resistance in Wild Tomato" International Journal of Molecular Sciences 24, no. 3: 2583. https://doi.org/10.3390/ijms24032583