Pharmacological Actions of 5-Hydroxyindolin-2 on Modulation of Platelet Functions and Thrombus Formation via Thromboxane A2 Inhibition and cAMP Production
Abstract
:1. Introduction
2. Results
2.1. 5-HI Blocks Platelet Activity, Cytotoxicity, and Half Maximal Inhibitory Concentration (IC50)
2.2. 5-HI Blocks [Ca2+]i Levels, IP3RI-, ERK-Phosphorylation, and Granule Release
2.3. 5-HI Blocks Thromboxane B2 Production and Dephosphorylation of cPLA2, p38MAPK
2.4. 5-HI Blocks Fibronectin Adhesion, Fibrinogen Binding, VASP, PI3K, and Akt Phosphoryation
2.5. 5-HI Elevated Cyclic Nucleotides and Suppressed Clot Retraction
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Human Platelet Aggregation
4.3. Cytotoxicity Analysis
4.4. Ca2+ Analysis
4.5. Serotonin and ATP Analysis
4.6. Thromboxane B2 Analysis
4.7. Immunoblotting Analysis
4.8. Fibronectin Adhesion Analysis
4.9. αIIb/β3 Activity Analysis
4.10. Fibrin Clot Retraction
4.11. Data Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kwon, H.-W.; Kim, S.D.; Rhee, M.H.; Shin, J.-H. Pharmacological Actions of 5-Hydroxyindolin-2 on Modulation of Platelet Functions and Thrombus Formation via Thromboxane A2 Inhibition and cAMP Production. Int. J. Mol. Sci. 2022, 23, 14545. https://doi.org/10.3390/ijms232314545
Kwon H-W, Kim SD, Rhee MH, Shin J-H. Pharmacological Actions of 5-Hydroxyindolin-2 on Modulation of Platelet Functions and Thrombus Formation via Thromboxane A2 Inhibition and cAMP Production. International Journal of Molecular Sciences. 2022; 23(23):14545. https://doi.org/10.3390/ijms232314545
Chicago/Turabian StyleKwon, Hyuk-Woo, Sung Dae Kim, Man Hee Rhee, and Jung-Hae Shin. 2022. "Pharmacological Actions of 5-Hydroxyindolin-2 on Modulation of Platelet Functions and Thrombus Formation via Thromboxane A2 Inhibition and cAMP Production" International Journal of Molecular Sciences 23, no. 23: 14545. https://doi.org/10.3390/ijms232314545