Inhibition of Recruitment and Activation of Neutrophils by Pyridazinone-Scaffold-Based Compounds
Abstract
:1. Introduction
2. Results
2.1. Pyridazinone-Scaffold-Based Compounds Decrease the Release of Pro-Inflammatory Mediators by Activated PMNs
2.2. Pyridazinone-Scaffold-Based Compounds Do Not Impact Beneficial Functions in Activated PMNs
2.3. Pyridazinone-Scaffold-Based Compounds Modulate PMN Phenotype after Transepithelial Migration
2.4. Pyridazinone-Scaffold-Based Compounds Decrease PMNs Recruitment In Vivo
3. Discussion
4. Materials and Methods
4.1. Media and Reagents
4.2. Compound Preparation
4.3. Collection of Blood Samples, Cell Isolation and Culture
4.4. ELISA
4.5. Gelatin Zymography
4.6. RNA Purification and Reverse-Transcription
4.7. Phagocytosis
4.8. Oxidative Stress Quantification
4.9. Transepithelial Migration
4.10. Air Pouch Model of PMN-Driven Tissue Inflammation
4.11. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Target Gene | Sequences | Efficiency | |
---|---|---|---|
Forward Primer (5′⟶3′) | Reverse Primer (5′⟶3′) | ||
MMP9 | GAACCAATCTCACCGACAGG | GCCACCCGAGTGTAACCATA | 1.94 |
CXCL8 | AGACAGCAGAGCACACAAGC | CTCCTTGGCAAAACTGCAC | 1.94 |
TNF | CAGCCTCTTCTCCTTCCTGAT | GCCAGAGGGCTGATTAGAGA | 1.95 |
RPS18 | TGCGAGTACTCAACACCAACA | GCATATCTTCGGCCCACA | 1.96 |
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Moniot, A.; Braux, J.; Siboni, R.; Guillaume, C.; Audonnet, S.; Allart-Simon, I.; Sapi, J.; Tirouvanziam, R.; Gérard, S.; Gangloff, S.C.; et al. Inhibition of Recruitment and Activation of Neutrophils by Pyridazinone-Scaffold-Based Compounds. Int. J. Mol. Sci. 2022, 23, 7226. https://doi.org/10.3390/ijms23137226
Moniot A, Braux J, Siboni R, Guillaume C, Audonnet S, Allart-Simon I, Sapi J, Tirouvanziam R, Gérard S, Gangloff SC, et al. Inhibition of Recruitment and Activation of Neutrophils by Pyridazinone-Scaffold-Based Compounds. International Journal of Molecular Sciences. 2022; 23(13):7226. https://doi.org/10.3390/ijms23137226
Chicago/Turabian StyleMoniot, Aurélie, Julien Braux, Renaud Siboni, Christine Guillaume, Sandra Audonnet, Ingrid Allart-Simon, Janos Sapi, Rabindra Tirouvanziam, Stéphane Gérard, Sophie C. Gangloff, and et al. 2022. "Inhibition of Recruitment and Activation of Neutrophils by Pyridazinone-Scaffold-Based Compounds" International Journal of Molecular Sciences 23, no. 13: 7226. https://doi.org/10.3390/ijms23137226