Transcriptome Analysis Reveals Sexual Disparities between Olfactory and Immune Gene Expression in the Olfactory Epithelium of Megalobrama amblycephala
Abstract
:1. Introduction
2. Results
2.1. The Structure of the Olfactory Epithelium
2.2. The SEM of the Olfactory Epithelium
2.3. Transcriptome Analysis
2.4. Differentially Expressed Genes
2.5. The Olfactory Receptor Repertoires
2.6. Regulatory Network of Immune Genes
2.7. Analysis of Differentially Expressed Transcription Factors
2.8. Quantitative Real-Time PCR (qPCR)
2.9. Fluorescence In Situ Hybridization
3. Discussion
4. Materials and Methods
4.1. Sample Collection
4.2. RNA Extraction and BGISEQ Library Construction
4.3. Analysis of RNA-Seq Data
4.4. Data Mining for the Olfactory Receptor Genes
4.5. Gene Network Analyses
4.6. Quantitative Real-Time PCR (qPCR)
4.7. Fluorescence In Situ Hybridization
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lv, M.; Chen, X.; Huang, X.; Liu, N.; Wang, W.; Liu, H. Transcriptome Analysis Reveals Sexual Disparities between Olfactory and Immune Gene Expression in the Olfactory Epithelium of Megalobrama amblycephala. Int. J. Mol. Sci. 2021, 22, 13017. https://doi.org/10.3390/ijms222313017
Lv M, Chen X, Huang X, Liu N, Wang W, Liu H. Transcriptome Analysis Reveals Sexual Disparities between Olfactory and Immune Gene Expression in the Olfactory Epithelium of Megalobrama amblycephala. International Journal of Molecular Sciences. 2021; 22(23):13017. https://doi.org/10.3390/ijms222313017
Chicago/Turabian StyleLv, Maolin, Xiuli Chen, Xin Huang, Ning Liu, Weimin Wang, and Han Liu. 2021. "Transcriptome Analysis Reveals Sexual Disparities between Olfactory and Immune Gene Expression in the Olfactory Epithelium of Megalobrama amblycephala" International Journal of Molecular Sciences 22, no. 23: 13017. https://doi.org/10.3390/ijms222313017