Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development
Abstract
:1. Introduction
2. Results
2.1. Hand1 Expression Decreases in a Dose-Sensitive Manner in Response to Bmp2 and Bmp4 Deficiency during Heart Development
2.2. Hand1 is Upregulated in Bmp4 OE Embryos
2.3. Hand1 Is a Direct Target Activated by the Canonical Bmp/Smad Signaling
2.4. Bmp Induces Hand1 Expression during Cardiomyogenesis in P19 Cells
3. Discussion
4. Materials and Methods
4.1. Mouse Alleles and Transgenic Lines
4.2. Antibodies and Reagents
4.3. Whole-Mount In Situ Hybridization
4.4. Quantitative Real Time RT-PCR
4.5. Chromatin Immunoprecipitation
4.6. Luciferase Reporter Assays
4.7. Western Blotting
4.8. Cell Culture
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zheng, M.; Erhardt, S.; Ai, D.; Wang, J. Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development. Int. J. Mol. Sci. 2021, 22, 9835. https://doi.org/10.3390/ijms22189835
Zheng M, Erhardt S, Ai D, Wang J. Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development. International Journal of Molecular Sciences. 2021; 22(18):9835. https://doi.org/10.3390/ijms22189835
Chicago/Turabian StyleZheng, Mingjie, Shannon Erhardt, Di Ai, and Jun Wang. 2021. "Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development" International Journal of Molecular Sciences 22, no. 18: 9835. https://doi.org/10.3390/ijms22189835