Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer
Abstract
:1. Introduction
2. Results
2.1. Tumor Microenvironment Influences EC Morphology, Proliferation and Expression of BCL6/p53
2.2. HBH.MEC and HBCa.MEC Lines Display ECs Phenotype
2.3. Hypoxia Induces the Production of VEGF-A by HBH.MEC and HBCa.MEC
2.4. Proangiogenic Response In Vitro Depends on the Pathological State of ECs
2.5. The Transcriptome Analysis of Breast Tumor-Derived ECs Compared with Healthy ECs Revealed a Distinct Expression Profile Implicated in Vascular Development and Angiogenesis
3. Discussion
4. Materials and Methods
4.1. Endothelial Cell Lines Culture
4.2. Cell Viability Assay
4.3. Flow Cytometry Analysis
4.4. Western Blotting
4.5. Next Generation Sequencing
4.6. Secretion of VEGF-A
4.7. In Vitro Angiogenesis Assay
4.8. Permeability Test
4.9. Cell Proliferation Assay
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wilkus, K.; Brodaczewska, K.; Kajdasz, A.; Kieda, C. Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer. Int. J. Mol. Sci. 2021, 22, 8862. https://doi.org/10.3390/ijms22168862
Wilkus K, Brodaczewska K, Kajdasz A, Kieda C. Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer. International Journal of Molecular Sciences. 2021; 22(16):8862. https://doi.org/10.3390/ijms22168862
Chicago/Turabian StyleWilkus, Kinga, Klaudia Brodaczewska, Arkadiusz Kajdasz, and Claudine Kieda. 2021. "Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer" International Journal of Molecular Sciences 22, no. 16: 8862. https://doi.org/10.3390/ijms22168862