Rice PIN Auxin Efflux Carriers Modulate the Nitrogen Response in a Changing Nitrogen Growth Environment
Abstract
:1. Introduction
2. Results
2.1. Identification and Functional Assignment of Rice PIN Family Members in Various Tissues/Organs
2.2. Morphological Appearance of Rice Seedlings under Conditions of Nitrogen Depletion and Ammonium Supplementation
2.3. OsPIN-Family Genes Are Closely Associated with Ammonium Treatment in Rice
2.4. Ammonium Resupply Decreases Auxin Response in Root Tips and Lateral Roots via the DR5::VENUS System
2.5. The Ospin1b Mutant (3A-04335) Displayed No Response to Ammonium Supplementation after Long-Term N Deficiency
2.6. Mutation of OsPIN1b Alters the Expression Pattern of Ammonium-Response-Related Genes
3. Discussion
3.1. Roles of PIN-Family Genes in Rice
3.2. Auxin Is Involved in the Ammonium-Dependent Response Mediated by Rice PIN-Family Genes Encoding Auxin Efflux Carriers
3.3. OsPIN1b Is Involved in Ammonium Assimilation
4. Conclusions
5. Materials and Methods
5.1. Multiple Sequence Alignment and Phylogenetic Analysis
5.2. Meta-Analysis of Organ-Specific Expression Profiles
5.3. Plant Material and Stress Treatment
5.4. RNA extracTion and Real-Time PCR
5.5. Genotyping of T-DNA Insertional Lines for the OsPIN1b Gene
5.6. IAA Distribution Assay Using the DR5:GFP System (DR5::VENUS line) in Response to Ammonium
5.7. Measurement of Chlorophyll Content
5.8. Measurement of Ammonium
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
AXR1 | Auxin-Resistant 1 |
GDH | Glutamate Dehydrogenase |
GS-GOGAT | Glutamine Synthase-Glutamate Synthase |
IDD10 | Indeterminate Domain |
OsAMT1;1~3 | Rice Ammonium Transporter 1;1~3 |
OsAS2 | Rice Asparagine Synthetase |
OsAUX1 | Rice Auxin Influx Carrier |
OsGDH2 | Rice Glutamate Dehydrogenase |
OsGLU3 | Rice endo-1, 4-b-D-glucanase 3 |
OsGS1;2 | Rice GLUTAMINE SYNTHETASE 1;2 |
OsNADH-GOGAT1 | Rice NADH Glutamate Synthase |
OsPIN | Rice Auxin Efflux Carriers |
OsUBI5 | Rice Ubiquitin 5 |
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Gho, Y.-S.; Song, M.-Y.; Bae, D.-Y.; Choi, H.; Jung, K.-H. Rice PIN Auxin Efflux Carriers Modulate the Nitrogen Response in a Changing Nitrogen Growth Environment. Int. J. Mol. Sci. 2021, 22, 3243. https://doi.org/10.3390/ijms22063243
Gho Y-S, Song M-Y, Bae D-Y, Choi H, Jung K-H. Rice PIN Auxin Efflux Carriers Modulate the Nitrogen Response in a Changing Nitrogen Growth Environment. International Journal of Molecular Sciences. 2021; 22(6):3243. https://doi.org/10.3390/ijms22063243
Chicago/Turabian StyleGho, Yun-Shil, Min-Yeong Song, Do-Young Bae, Heebak Choi, and Ki-Hong Jung. 2021. "Rice PIN Auxin Efflux Carriers Modulate the Nitrogen Response in a Changing Nitrogen Growth Environment" International Journal of Molecular Sciences 22, no. 6: 3243. https://doi.org/10.3390/ijms22063243