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Article

PEG-8 Laurate Fermentation of Staphylococcus epidermidis Reduces the Required Dose of Clindamycin Against Cutibacterium acnes

by
Shinta Marito
1,
Sunita Keshari
2 and
Chun-Ming Huang
1,*
1
Department of Biomedical Sciences and Engineering, National Central University, Taoyuan 32001, Taiwan
2
Department of Life Sciences, National Central University, Taoyuan 32001, Taiwan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(14), 5103; https://doi.org/10.3390/ijms21145103
Submission received: 12 June 2020 / Revised: 12 July 2020 / Accepted: 17 July 2020 / Published: 19 July 2020
(This article belongs to the Special Issue Skin Microbiome)
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Abstract

:
The probiotic activity of skin Staphylococcus epidermidis (S. epidermidis) bacteria can elicit diverse biological functions via the fermentation of various carbon sources. Here, we found that polyethylene glycol (PEG)-8 Laurate, a carbon-rich molecule, can selectively induce the fermentation of S. epidermidis, not Cutibacterium acnes (C. acnes), a bacterium associated with acne vulgaris. The PEG-8 Laurate fermentation of S. epidermidis remarkably diminished the growth of C. acnes and the C. acnes-induced production of pro-inflammatory macrophage-inflammatory protein 2 (MIP-2) cytokines in mice. Fermentation media enhanced the anti-C. acnes activity of a low dose (0.1%) clindamycin, a prescription antibiotic commonly used to treat acne vulgaris, in terms of the suppression of C. acnes colonization and MIP-2 production. Furthermore, PEG-8 Laurate fermentation of S. epidermidis boosted the activity of 0.1% clindamycin to reduce the sizes of C. acnes colonies. Our results demonstrated, for the first time, that the PEG-8 Laurate fermentation of S. epidermidis displayed the adjuvant effect on promoting the efficacy of low-dose clindamycin against C. acnes. Targeting C. acnes by lowering the required doses of antibiotics may avoid the risk of creating drug-resistant C. acnes and maintain the bacterial homeostasis in the skin microbiome, leading to a novel modality for the antibiotic treatment of acne vulgaris.

1. Introduction

The skin is the largest organ, and also home to an ecosystem of a diverse milieu of aerobic and anaerobic microorganisms, most of which are harmless or even beneficial to their host. Microorganisms in the skin microbiome play a chief role in educating the immune system by modulating the cutaneous innate and adaptive immune system [1]. Dysbiosis by microbial imbalance can largely influence its colonization niche by altering the functions of innate and adaptive immunity [2]. A robust evidence for a dysbiotic microbiome is well-documented in inflammatory skin conditions, such as psoriasis, where patients exhibit substantial increases in Staphylococcus species on lesional skin. A temporal shift in the composition of the skin microbiome was characterized by significant increases in Staphylococcus levels in the skin of atopic dermatitis (AD) patients [3]. Overgrowth of skin commensal Cutibacterium acnes (C. acnes) (formally named as Propionibacterium acnes) has been linked to the cause of inflammatory acne vulgaris, which affects an estimated 80% of Americans. About 94–95% of the pubertal population, 20–40% of adults and <25% of women suffered from acne vulgaris [4]. The study evidenced that C. acnes may activate Toll-like receptor (TLR)-2 and TLR-4 on membranes of inflammatory cells releasing inflammatory cytokines, including tumor necrosis factor (TNF)-α and interleukin (IL)-1, IL-8, and IL-12 [5].
Bacterial interference has been found in commensal bacteria, which mediated fermentation to prevent the colonization of pathogens on the host. For example, short-chain fatty acids (SCFAs) or ethanol produced from microbial fermentation of sugars on and inside fruits inhibited the growth of bacterial competitors within ripe fruits [6]. Therapeutic application of bacterial interference by active colonization using Staphylococcus epidermidis (S. epidermidis), a commensal bacterium in humans, was successful in counteracting the infection of Staphylococcus aureus (S. aureus) [7,8,9]. Published results from our lab showed that commensal S. epidermidis in the skin can ferment glycerol, sucrose or polyethylene glycol (PEG) to produce SCFAs, such as acetic, butyric, lactic, and succinic acids, and successfully inhibited growth of opportunistic C. acnes [10,11,12,13]. Although antibiotics have been a common treatment for acne vulgaris for many years, they exert selection pressure on non-target bacteria in skin, running a risk of developing antibiotic-resistant C. acnes [14,15]. The incidence of C. acnes antibiotic resistance has increased from 20% to 72.5% in 1995, showing that widespread resistance has become a major dermatological issue [16,17]. Major factors, like the chronicity of the disease, antimicrobial administration route, effective penetration in the skin, the duration of therapy, poor treatment compliance, and easy access to therapeutic agents without medical supervision have contributed to the development of antibiotic resistance C. acnes. Further, antibiotic-resistant C. acnes may also spread from patients to close contacts and is found to be associated with a poor treatment outcome [18,19,20,21]. To reduce the dose of antibiotic, the combination of antibiotic with its adjuvant may be a better strategy to suppress the emergence of resistance and rescue the activity of existing drugs, with little or no antibiotic activity themselves [22]. Poly ethylene glycol (PEG) are most widely used stealth polymer in drug delivery and cosmetics, due to their easy solubility and higher viscosity [23]. PEG has The International Nomenclature of Cosmetic Ingredients (INCI) name, and is thus regulated as safe for use in cosmetics. Moreover, PEGs have a wide variety of PEG-derived mixtures due to their readily linkable terminal primary hydroxyl groups in combination with many possible compounds or complexes, such as ethers or fatty acids [24]. A study revealed that α-PEG Abs was elicited by immunization with PEG coated or PEGylated proteins. However, a little or no immunogenicity was detected by administration with PEG alone suggesting induction of PEG-specific immunity can occur in the absence of adjuvants [25,26]. Moreover, research investigated PEG as a safe adjuvant for HIV and hepatitis B virus DNA vaccines [26,27].
In this study, the polyoxyethylene glycol 400 monolaurate labeled as PEG-8 Laurate (C28H56O10) by INCI was used as a carbon source to induce the fermentation of S. epidermidis. We found that PEG-8 Laurate fermentation of S. epidermidis can lower the required dose of clindamycin against C. acnes, demonstrating the adjuvant effect of PEG-8 Laurate on the anti-C. acnes activity of clindamycin.

2. Results

2.1. PEG-8 Laurate as a Selective Carbon Source for Fermentation of S. epidermidis

To investigate whether S. epidermidis ATCC 12228 and C. acnes ATCC 6919 can ferment PEG-8 Laurate, bacteria (105 CFU) were incubated with and without 2% PEG-8 Laurate in rich media containing phenol red for 12 h. Rich media with PEG-8 Laurate alone or bacteria alone served as controls. 2% PEG-8 Laurate did not influence the bacterial growth during a 12 h incubation (Figure S1). The color of phenol red in rich media containing S. epidermidis, but not C. acnes, in the presence of PEG-8 Laurate changed from red to yellow after 12 h incubation, indicating that PEG-8 Laurate is a selective carbon source for the fermentation of S. epidermidis (Figure 1a,c). The color change of phenol red was quantified by measuring the optical density at 560 nm (OD560) (Figure 1b,d).

2.2. PEG-8 Laurate Fermentation of S. epidermidis Against C. acnes In Vivo

To evaluate whether PEG-8 Laurate fermentation of S. epidermidis can influence the growth of C. acnes in vivo, S. epidermidis (107 CFU) and C. acnes (107 CFU), with or without 2% PEG-8 Laurate, were co-injected intradermally into ear of Institute for Cancer Research (ICR) mice for five days. In Figure 2, compared to those mice injected with two bacteria plus H2O, mice injected with two bacteria plus PEG-8 Laurate showed a decrease in both ear redness and thickness (Figure 2a,b). The number (CFU/mL) of C. acnes in mouse ears injected with two bacteria in the presence of PEG-8 Laurate was approximately one log10 lower than that in ears injected with two bacteria in the absence of PEG-8 Laurate (Figure 2c). Since acne vulgaris is an inflammatory skin disorder [5], we next determined whether PEG-8 Laurate fermentation of S. epidermidis can ameliorate the production of C. acnes-induced pro-inflammatory cytokines. The mouse ears were excised and homogenized five days after bacterial injection. The level of macrophage-inflammatory protein-2 (MIP-2), a murine counterpart of human IL-8 [13], in ear homogenates was measured by an enzyme-linked immunosorbent assay (ELISA). As shown in Figure 2d, the level of MIP-2 in the ear injected with two bacteria plus PEG-8 Laurate was significantly lower than that in the ear injected with two bacteria and H2O (Figure 2d). Furthermore, in terms of the number of C. acnes and level of MIP-2, we found that there were no differences in mouse ears injected, with or without 2% PEG-8 Laurate, alone in the absence of bacteria, demonstrating that PEG-8 Laurate also have no impact on C. acnes growth and MIP-2 production (Figure S2). The results in Figure 2 suggested that S. epidermidis mediated PEG-8 Laurate for fermentation, to mitigate the growth of C. acnes and inflammation.

2.3. Reduction of the Effective Dose of Clindamycin Against C. acnes by Fermentation Media

Clindamycin is one of the common antibiotics for acne vulgaris [28]. However, the antibiotic single-agent therapy can result in the rapid development of clinically significant antibiotic resistance [28]. Several carbohydrates, for example sucrose, have been added into antibiotic (amoxicillin) formulae as ingredients [29]. An adjuvant is a pharmacological or immunological agent that modifies the effect of a drug or vaccine [29,30]. To evaluate the effect of PEG-8 Laurate fermentation on the efficacies of anti-C. acnes antibiotics, we injected the ears of ICR mice intradermally with C. acnes (107 CFU) with 0.1%, 1% clindamycin or H2O, along with media collected from the culture of S. epidermidis in the presence or absence of 2% PEG-8 Laurate. Six days after injection, ears were excised and homogenized for the quantification of the number of C. acnes and the level of MIP-2. As shown in Figure 3, the injection of 1%, but not 0.1% clindamycin, along with culture media of S. epidermidis, caused a remarkable decrease in the number of C. acnes and level of MIP-2. However, the 0.1% clindamycin became effective in terms of reduction of the number of C. acnes (Figure 3b) and level of MIP-2 (Figure 3c), when it was co-injected with fermentation media of S. epidermidis in the presence of PEG-8 Laurate. The number of C. acnes in ears injected with C. acnes/0.1% clindamycin and media of S. epidermidis plus PEG-8 Laurate was one log10 lower than that in ears injected with C. acnes/0.1% clindamycin and media of S. epidermidis. The levels of MIP-2 in ears injected with C. acnes/0.1% clindamycin and media of S. epidermidis with or without PEG-8 Laurate are 3469 ± 26.61 and 7685 ± 561.5 ng/mL, respectively. These results indicated that molecules in fermentation media could enhance the efficacy of clindamycin against C. acnes.

2.4. Enhancement of Low Dose of Clindamycin for Inhibition of the Growth of C. acnes Colonies by PEG-8 Laurate Fermentation

To confirm that the PEG-8 Laurate fermentation of S. epidermidis can augment the activity of low dose of clindamycin against C. acnes, C. acnes in the presence of 0.1% or 1% clindamycin was added onto the top of a homogeneous lawn of S. epidermidis, with or without 2% PEG-8 Laurate in agar plates. As shown in Figure 4, the addition of 0.1% clindamycin with C. acnes onto the S. epidermidis lawn without PEG-8 Laurate can significantly reduce the size of a C. acnes colony. The C. acnes colony was nearly completely eliminated when 1% clindamycin with C. acnes was added onto the S. epidermidis lawn without PEG-8 Laurate. Moreover, 2% PEG-8 Laurate was supplemented into the S. epidermidis lawn to induce the S. epidermidis fermentation. An inhibition zone was observed at the border of a C. acnes colony on the S. epidermidis lawn with 2% PEG-8 Laurate (Figure 4a), illustrating the bacterial interference by PEG-8 Laurate fermentation of S. epidermidis. Supplementation of PEG-8 Laurate into the S. epidermidis lawn considerably enhanced the ability of 0.1% clindamycin to eradicate the C. acnes colony (Figure 4b). The result demonstrated that PEG-8 Laurate fermentation of S. epidermidis could boost the low dose of clindamycin against C. acnes.

3. Discussion

Different bacteria express distinct enzymes that metabolize carbon sources during fermentation [31]. Previous results from our laboratory have shown that S. epidermidis, but not C. acnes, can ferment sucrose to produce SCFAs [13]. Here, we found that PEG-8 Laurate could selectively induce fermentation of S. epidermidis, but not C. acnes (Figure 1). Thus, as carbon sources, sucrose and PEG-8 Laurate may function as prebiotics, which can specifically provoke the fermentation of S. epidermidis for the production of SCFAs. It has been reported that SCFAs such as succinic acid and acetic acid can effectively suppress the growth of C. acnes [13]. The fact that the production of SCFAs with anti-C. acnes activities in fermentation media of S. epidermidis may well explain why PEG-8 Laurate can lower the required dose of clindamycin for killing C. acnes. Our previous results have identified many SCFAs produced by PEG fermentation of S. epidermidis via gas chromatography mass spectrometry (GC-MS) analysis [12]. Future work will include the identification of SCFAs in the media of PEG-8 Laurate fermentation of S. epidermidis. Lauric acid or its derivative elicits antimicrobial activity against C. acnes [32]. However, our data demonstrated that 2% PEG-8 Laurate itself, as a lauric acid derivative, did not influence the growth of C. acnes (Figure S1). PEG-8 Laurate has been used in cosmetics and beauty products as a surfactant and emulsifying agent. Our study demonstrated, for the first time, that skin S. epidermidis bacteria can ferment PEG-8 Laurate to counteract C. acnes.
Adjuvants for antibiotics include small molecules, which can enhance the effects of antibiotics without killing the bacteria directly by themselves. Therapy using antibiotic adjuvants may be a potent way to suppress the formerly susceptible bacteria that have acquired resistance [33]. Furthermore, β-Lactamase inhibitors, in combination with β-lactam antibiotics, were found to be successful for the treatment of bacterial infections [34,35,36]. The successful inhibition of Acinetobacter baumannii, an extensively drug-resistant strain causing 50% mortality rates when causing ventilator-associated pneumonia, has been achieved by 2-aminoimidazole-based compounds, which disrupt two-component signaling, and anthracyclines, that potentiate the activity of rifampin and linezolid [37,38]. Antibiotic resistance is a major hurdle in the treatment of infectious diseases. Reduction of the frequency and intensity of antibiotic consumption may be a way to prevent the development of antibiotic resistant strains. Any product, pathway, or phenotype that contributes to decreased antibiotic susceptibility represents a potential adjuvant target, many of which have yet to be fully probed. Here, we demonstrated that the PEG-8 Laurate fermentation of S. epidermidis can reduce the effective dose of clindamycin for killing C. acnes (Figure 3 and Figure 4). PEG-8 Laurate, thus, functions as an adjuvant to promote the anti-C. acnes efficacy of clindamycin, by stimulating the probiotic activity of S. epidermidis. Detection of C. acnes induced inflammatory cytokines provides a validation on effective inhibition on C. acnes growth. CXCL1/keratinocyte-derived chemokine (KC), CXCL2/MIP2 and CXCL5-6/lipopolysaccharide-induced CXC chemokine (LIX) were regarded as functional homologues of IL-8, where CXCL1, CXCL2 and CXCL6 are identified as candidates associated with acne pathogenesis in humans [39,40]. Additionally, multiple studies in the mouse acne model showed a remarkable increase in the production of neutrophils, macrophages and MIP-2 upon C. acnes administration into mice [41,42,43]. In our study, we investigated that PEG-8 Laurate fermentation of S. epidermidis could potentially ameliorate the effective dose of clindamycin to reduce C. acnes-induced MIP-2 production (Figure 3). Future studies involving the detection of CXCL1 and CXCL6 in murine model of acne may provide additional information to correlate these inflammatory mediators with acne severity.
Over the years, an increased antibiotic resistance to C. acnes was detected to tetracycline and clindamycin. The prevalent rates of C. acnes with erythromycin or tetracycline resistance ranges from 5% to 26.4%, however 45% to 91% clindamycin resistance was detected in acne vulgaris [44]. Although at earlier phases, the efficacy of in acne treatment has been shown to be sustained, there was still an increase in the number of resistant bacteria in samples from patients using 1% clindamycin alone [45]. Although the spectrum of antimicrobial activity of clindamycin includes staphylococci, streptococci and pneumococci and most anaerobic bacteria [46], our result showed that 1% clindamycin can efficiently kill anaerobic C. acnes, but not S. epidermidis, a facultative anaerobic bacterium (Figure S3). The 0.1% clindamycin did not influence the interference of S. epidermidis with C. acnes in the absence of PEG-8 Laurate (Figure 3), although it can significantly reduce the size of a C. acnes colony in a S. epidermidis lawn (Figure 4). The supplementation of PEG-8 Laurate into a S. epidermidis lawn significantly enhanced the anti-C. acnes activity of 0.1% clindamycin. Clindamycin acts by inhibiting the protein synthesis of bacteria at the level of the 50S ribosome. PEG-8 Laurate initiated S. epidermidis fermentation and may induce the production of SCFAs, which can lower the intracellular pH values of bacteria [11], leading the lysis of C. acnes. The INCI name of PEG-8 and its efficacy as a potential prebiotic made it safer for extensive usage in cosmetics and drugs [12,24]. In this study, C. acnes ATCC 6919, a phylotype IA1, was used to demonstrate the adjuvant effect of PEG-8 Laurate fermentation of S. epidermidis against C. acnes. The phylotype IA1 of C. acnes was predominantly associated with acne vulgaris. The phylotype IA1 of C. acnes displayed clindamycin sensitivity, whereas phylotype IA2 frequently associated with severe acne vulgaris and phylotype IB associated with commensal C. acnes exhibited high clindamycin resistance [47]. Future studies will thus investigate the effect of PEG-8 Laurate fermentation of S. epidermidis on clindamycin against phylotypes IA2 and IB of C. acnes.
In summary, a “drug repurposing” strategy unveils many compounds that have the unexpected potential to treat different diseases. Although PEG-8 Laurate is currently used as an emulsifying agent in cosmetic products, we repurpose it as a prebiotic, which can induce the fermentation of skin probiotic S. epidermidis. PEG-8 Laurate fermentation of S. epidermidis reduced the therapeutically effective dose of clindamycin, demonstrating the potential of PEG-8 Laurate as an antibiotic adjuvant for treatments of acne vulgaris and other skin infections.

4. Materials and Methods

4.1. Ethics Statement

This research was carried out in strict accordance with an approved Institutional Animal Care and Use Committee (IACUC) protocol at National Central University (NCU), Taiwan (NCU-106-016, 19 December 2017). ICR mice (8–9-week-old females) obtained from National Laboratory Animal Centre, Taipei, Taiwan, were sacrificed in dry ice (solid carbon dioxide) in a closed box.

4.2. Bacterial Culture

S. epidermidis ATCC 12228 bacteria were cultured in tryptic soy broth (TSB) (Sigma, St. Louis, MO, USA) overnight at 37 °C. C. acnes ATCC 6919 bacteria were cultured on Reinforced Clostridium Medium (RCM) (ThermoFisher Scientific, Waltham, MA, USA), and cultured under anaerobic conditions using a Gas-Pak (BD Biosciences, San Jose, CA, USA) at 37 °C, until the logarithmic growth phase. The cultures were diluted at 1:100 and OD was measured at 600 nm (OD600) = 1.0. Bacterial pellets were harvested by centrifugation at 5000× g for 10 min, washed with phosphate-buffered saline (PBS), and suspended in PBS for further experiments.

4.3. Fermentation of Bacteria

S. epidermidis or C. acnes (105 CFU/mL) was incubated in 10 mL rich media (10 g/L yeast extract (Biokar Diagnostics, Beauvais, France), 3 g/L TSB, 2.5 g/L K2HPO4, and 1.5 g/L KH2PO4), in the presence and absence of 20 g/L PEG-8 Laurate (Taiwan NJC Corporation), under aerobic/anaerobic conditions, at 37 °C overnight shaking at 200 rpm. In some experiments, 20 g/L PEG-8 Laurate was added into media without bacteria. The 0.002% (w/v) phenol red (Sigma) in rich media was served as a fermentation indicator. A color change from red to yellow indicated the occurrence of bacterial fermentation, and it was detected by measuring OD at 560 nm (OD560).

4.4. PEG-8 Laurate Fermentation of S. epidermidis Against C. acnes In Vivo

ICR mice were anesthetized by isoflurane (Panion and BF Biotech Inc., Taiwan). Three mice per group were used in each experiment. The ears of ICR mice were injected intradermally with C. acnes ATCC 6919 (107 CFU) and S. epidermidis ATCC 12228 (107 CFU), with or without 2% PEG-8 Laurate for 5 days. In some experiments, mouse ears were injected intradermally with C. acnes (107 CFU) and 0.1 or 1% clindamycin, along with media collected from the culture of S. epidermidis (107 CFU), in the presence or absence of 2% PEG-8 Laurate for 6 days. Thickness of mouse ear was measured every day with an electronic digital caliper (Mitutoyo, Kanagawa, Japan). Ear redness or erythema was photographed using a USB digital microscope (GM019-#1, Global Camera Manufacturer Corp, Taipei, Taiwan). Mouse ears were excised and homogenized for detection of MIP-2 and bacterial counts 5 or 6 days after bacterial injection.

4.5. Bacterial Loads in Mouse Ears

After excising mouse ears, tissue homogenates were made by a tissue grinder in 200 μL of sterile PBS. CFUs of C. acnes in ear homogenates were enumerated by plating serial dilutions (1:100–1:105) of homogenates on C. acnes selective agar plate containing RCM and 10µg/mL of furazolidone (PubChem, Rockville Pike, Bethesda, MD, USA). Plates were incubated for 3 days at 37 °C under an anaerobic condition, using a Gas-Pak.

4.6. An Overlay Assay

S. epidermidis ATCC 12228 (108 CFU) in 300 µL PBS was poured into the plates, containing 2% RCM agar, with or without 2% PEG -8 Laurate, to produce a homogeneous lawn of S. epidermidis. Furthermore, C. acnes ATCC6919 (106 CFU), with or without 0.1 and 1% clindamycin in 10 µL water, was added on the top of the lawn of S. epidermidis before incubation at 37 °C for 5 days. Addition of C. acnes without clindamycin was included as a control.

4.7. ELISA

Mouse ears were excised and homogenized with T-PERTM Tissue Protein Extraction Reagent (ThermoFisher Scientific), supplemented with ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Sigma). Ear homogenates were centrifuged at 15,000 rpm for 30 min at 4 °C and supernatant was collected to measure MIP-2 concentration by sandwich ELISA, using a Quantikine mouse MIP-2 set (R&D Systems, Minneapolis, MN, USA).

4.8. Statistical Analysis

Data analysis was performed by unpaired Student’s t-test or by 1-way ANOVA using Prism software (GraphPad Software, La Jolla, CA, USA). The bacterial inhibition zone in the overlay assay was measured using ImageJ software (National Institutes of Health, Bethesda, MD, USA). The p-values of <0.05 (*), <0.01 (**), and <0.001 (***) were considered as significant. The mean ± SD for at least three independent experiments was calculated.

Supplementary Materials

The following are available online at https://www.mdpi.com/1422-0067/21/14/5103/s1.

Author Contributions

S.M. and S.K. performed experiments and wrote this manuscript. C.-M.H. designed all experiments, wrote and edited this manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This study was supported by 106/107/108-Landseed Hospital-NCU joint grants and Ministry of Science and Technology (MOST) Grants 108-2622-B-008-001-CC1; 108-2314-B-008-003-MY3, and 107-2923-B-008-001-MY3.

Conflicts of Interest

All co-authors do not have any financial conflicts of interest.

References

  1. Grice, E.A.; Segre, J.A. The skin microbiome. Nat. Rev. Microbiol. 2011, 9, 244. [Google Scholar] [CrossRef] [PubMed]
  2. Levy, M.; Kolodziejczyk, A.A.; Thaiss, C.A.; Elinav, E. Dysbiosis and the immune system. Nat. Rev. Immunol. 2017, 17, 219. [Google Scholar] [CrossRef] [PubMed]
  3. Johnson, M.E.; Franks, J.M.; Cai, G.; Mehta, B.K.; Wood, T.A.; Archambault, K.; Pioli, P.A.; Simms, R.W.; Orzechowski, N.; Arron, S. Microbiome dysbiosis is associated with disease duration and increased inflammatory gene expression in systemic sclerosis skin. Arthritis Res. Ther. 2019, 21, 49. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Kanwar, I.L.; Haider, T.; Kumari, A.; Dubey, S.; Jain, P.; Soni, V. Models for acne: A comprehensive study. Drug Discov. Ther. 2018, 12, 329–340. [Google Scholar] [CrossRef] [Green Version]
  5. Dreno, B.; Gollnick, H.; Kang, S.; Thiboutot, D.; Bettoli, V.; Torres, V.; Leyden, J.; Global Alliance to Improve Outcomes in Acne. Understanding innate immunity and inflammation in acne: Implications for management. J. Eur. Acad. Dermatol. Venereol. 2015, 29, 3–11. [Google Scholar] [CrossRef] [Green Version]
  6. Dudley, R. Ethanol, fruit ripening, and the historical origins of human alcoholism in primate frugivory. Integr. Comp. Biol. 2004, 44, 315–323. [Google Scholar] [CrossRef] [Green Version]
  7. Iwase, T.; Uehara, Y.; Shinji, H.; Tajima, A.; Seo, H.; Takada, K.; Agata, T.; Mizunoe, Y. Staphylococcus epidermidis Esp inhibits Staphylococcus aureus biofilm formation and nasal colonization. Nature 2010, 465, 346. [Google Scholar] [CrossRef]
  8. Lai, Y.; Di Nardo, A.; Nakatsuji, T.; Leichtle, A.; Yang, Y.; Cogen, A.L.; Wu, Z.-R.; Hooper, L.V.; Schmidt, R.R.; Von Aulock, S. Commensal bacteria regulate Toll-like receptor 3–dependent inflammation after skin injury. Nat. Med. 2009, 15, 1377. [Google Scholar] [CrossRef]
  9. Otto, M. Staphylococcus epidermidis—The’accidental’pathogen. Nat. Rev. Microbiol. 2009, 7, 555. [Google Scholar] [CrossRef] [Green Version]
  10. Yang, A.-J.; Marito, S.; Yang, J.-J.; Keshari, S.; Chew, C.-H.; Chen, C.-C.; Huang, C.-M. A Microtube Array Membrane (MTAM) encapsulated live fermenting staphylococcus epidermidis as a skin probiotic patch against cutibacterium acnes. Int. J. Mol. Sci. 2019, 20, 14. [Google Scholar] [CrossRef] [Green Version]
  11. Shu, M.; Wang, Y.; Yu, J.; Kuo, S.; Coda, A.; Jiang, Y.; Gallo, R.L.; Huang, C.-M. Fermentation of Propionibacterium acnes, a commensal bacterium in the human skin microbiome, as skin probiotics against methicillin-resistant Staphylococcus aureus. PLoS ONE 2013, 8, e55380. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Kao, M.S.; Huang, S.; Chang, W.L.; Hsieh, M.F.; Huang, C.J.; Gallo, R.L.; Huang, C.M. Microbiome precision editing: Using PEG as a selective fermentation initiator against methicillin-resistant Staphylococcus aureus. Biotechnol. J. 2017. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Wang, Y.; Kao, M.-S.; Yu, J.; Huang, S.; Marito, S.; Gallo, R.; Huang, C.-M. A precision microbiome approach using sucrose for selective augmentation of Staphylococcus epidermidis fermentation against Propionibacterium acnes. Int. J. Mol. Sci. 2016, 17, 1870. [Google Scholar] [CrossRef] [Green Version]
  14. Schmidt, N.W.; Agak, G.W.; Deshayes, S.; Yu, Y.; Blacker, A.; Champer, J.; Xian, W.; Kasko, A.M.; Kim, J.; Wong, G.C. Pentobra: A potent antibiotic with multiple layers of selective antimicrobial mechanisms against Propionibacterium acnes. J. Investig. Dermatol. 2015, 135, 1581–1589. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Keshari, S.; Kumar, M.; Balasubramaniam, A.; Chang, T.-W.; Tong, Y.; Huang, C.-M. Prospects of acne vaccines targeting secreted virulence factors of Cutibacterium acnes. Expert Rev. Vaccines 2019, 18, 433–437. [Google Scholar] [CrossRef] [PubMed]
  16. Humphrey, S. Antibiotic resistance in acne treatment. Skin Ther. Lett. 2012, 17, 1–3. [Google Scholar]
  17. Williams, H.C.; Dellavalle, R.P.; Garner, S. Acne vulgaris. Lancet 2012, 379, 361–372. [Google Scholar] [CrossRef]
  18. Eady, E.; Gloor, M.; Leyden, J. Propionibacterium acnes resistance: A worldwide problem. Dermatology 2003, 206, 54–56. [Google Scholar] [CrossRef]
  19. Ross, J.; Snelling, A.; Carnegie, E.; Coates, P.; Cunliffe, W.; Bettoli, V.; Tosti, G.; Katsambas, A.; Galvan Perez Del Pulgar, J.; Rollman, O. Antibiotic-resistant acne: Lessons from Europe. Br. J. Dermatol. 2003, 148, 467–478. [Google Scholar] [CrossRef]
  20. EADY, E.A.; Cove, J.; Holland, K.; Cunliffe, W. Erythromycin resistant propionibacteria in antibiotic treated acne patients: Association with therapeutic failure. Br. J. Dermatol. 1989, 121, 51–57. [Google Scholar] [CrossRef]
  21. Patel, M.; Bowe, W.P.; Heughebaert, C.; Shalita, A.R. The development of antimicrobial resistance due to the antibiotic treatment of acne vulgaris: A review. J. Drugs Dermatol. 2010, 9, 655–664. [Google Scholar] [PubMed]
  22. Wright, G.D. Antibiotic adjuvants: Rescuing antibiotics from resistance. Trends Microbiol. 2016, 24, 862–871. [Google Scholar] [CrossRef] [PubMed]
  23. Fruijtier, C. Safety assessment on polyethylene glycols (PEGs) and their derivatives as used in cosmetic products. Toxicology 2005, 214, 1–38. [Google Scholar] [CrossRef]
  24. Jang, H.-J.; Shin, C.Y.; Kim, K.-B. Safety Evaluation of Polyethylene Glycol (PEG) compounds for cosmetic use. Toxicol. Res. 2015, 31, 105–136. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Yang, Q.; Lai, S.K. Anti-PEG immunity: Emergence, characteristics, and unaddressed questions. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 2015, 7, 655–677. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  26. Liu, Y.; Balachandran, Y.L.; Li, D.; Shao, Y.; Jiang, X. Polyvinylpyrrolidone–poly(ethylene glycol) modified silver nanorods can be a safe, noncarrier adjuvant for HIV vaccine. ACS Nano 2016, 10, 3589–3596. [Google Scholar] [CrossRef] [PubMed]
  27. Wang, J.; Lu, L.; Song, H.; Yang, Y.; Ma, Y. Effect of polyethylene glycol as adjuvant on hepatitis B virus DNA vaccine in vitro. Acta Microbiol. Sin. 2010, 50, 949–954. [Google Scholar]
  28. Karadag, A.S.; Kayıran, M.A.; Wu, C.Y.; Chen, W.; Parish, L.C. Antibiotic resistance in acne: Changes, consequences and concerns. J. Eur. Acad. Dermatol. Venereol. 2020. [Google Scholar] [CrossRef]
  29. Soares, D.N.; Antonio, A.G.; Iorio, N.L.; Pierro, V.S.; Santos, K.R.; Maia, L.C. Does the presence of sucrose in pediatric antibiotics influence the enamel mineral loss and the Streptococcus mutans counts in dental biofilm? Braz. Dent. J. 2015, 26, 249–257. [Google Scholar] [CrossRef] [Green Version]
  30. Paul, M.; Carmeli, Y.; Durante-Mangoni, E.; Mouton, J.W.; Tacconelli, E.; Theuretzbacher, U.; Mussini, C.; Leibovici, L. Combination therapy for carbapenem-resistant Gram-negative bacteria. J. Antimicrob. Chemother. 2014, 69, 2305–2309. [Google Scholar] [CrossRef] [Green Version]
  31. Robbins, G.B.; Lewis, K.H. Fermentation of sugar acids by bacteria. J. Bacteriol. 1940, 39, 399. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  32. Nakatsuji, T.; Kao, M.C.; Fang, J.-Y.; Zouboulis, C.C.; Zhang, L.; Gallo, R.L.; Huang, C.-M. Antimicrobial property of lauric acid against Propionibacterium acnes: Its therapeutic potential for inflammatory acne vulgaris. J. Investig. Dermatol. 2009, 129, 2480–2488. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Melander, R.J.; Melander, C. The challenge of overcoming antibiotic resistance: An adjuvant approach? ACS Infect. Dis. 2017, 3, 559–563. [Google Scholar] [CrossRef]
  34. Papp-Wallace, K.M.; Bonomo, R.A. New beta-lactamase inhibitors in the clinic. Infect. Dis. Clin. N. Am. 2016, 30, 441–464. [Google Scholar] [CrossRef] [PubMed]
  35. Drawz, S.M.; Bonomo, R.A. Three decades of beta-lactamase inhibitors. Clin. Microbiol. Rev. 2010, 23, 160–201. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Bush, K. A resurgence of beta-lactamase inhibitor combinations effective against multidrug-resistant Gram-negative pathogens. Int. J. Antimicrob. Agents 2015, 46, 483–493. [Google Scholar] [CrossRef]
  37. Thompson, R.J.; Bobay, B.G.; Stowe, S.D.; Olson, A.L.; Peng, L.; Su, Z.; Actis, L.A.; Melander, C.; Cavanagh, J. Identification of BfmR, a response regulator involved in biofilm development, as a target for a 2-Aminoimidazole-based antibiofilm agent. Biochemistry 2012, 51, 9776–9778. [Google Scholar] [CrossRef] [Green Version]
  38. Cox, G.; Koteva, K.; Wright, G.D. An unusual class of anthracyclines potentiate Gram-positive antibiotics in intrinsically resistant Gram-negative bacteria. J. Antimicrob. Chemother. 2014, 69, 1844–1855. [Google Scholar] [CrossRef] [Green Version]
  39. Li, X.; Jia, Y.; Wang, S.; Meng, T.; Zhu, M. Identification of genes and pathways associated with acne using integrated bioinformatics methods. Dermatology 2019, 235, 445–455. [Google Scholar] [CrossRef]
  40. Kelhala, H.L.; Palatsi, R.; Fyhrquist, N.; Lehtimaki, S.; Vayrynen, J.P.; Kallioinen, M.; Kubin, M.E.; Greco, D.; Tasanen, K.; Alenius, H.; et al. IL-17/Th17 pathway is activated in acne lesions. PLoS ONE 2014, 9, e105238. [Google Scholar] [CrossRef]
  41. Nakatsuji, T.; Shi, Y.; Zhu, W.; Huang, C.P.; Chen, Y.R.; Lee, D.Y.; Smith, J.W.; Zouboulis, C.C.; Gallo, R.L.; Huang, C.M. Bioengineering a humanized acne microenvironment model: Proteomics analysis of host responses to Propionibacterium acnes infection in vivo. Proteomics 2008, 8, 3406–3415. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  42. Achermann, Y.; Goldstein, E.J.; Coenye, T.; Shirtliff, M.E. Propionibacterium acnes: From commensal to opportunistic biofilm-associated implant pathogen. Clin. Microbiol. Rev. 2014, 27, 419–440. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Itakura, M.; Tokuda, A.; Kimura, H.; Nagai, S.; Yoneyama, H.; Onai, N.; Ishikawa, S.; Kuriyama, T.; Matsushima, K. Blockade of secondary lymphoid tissue chemokine exacerbates Propionibacterium acnes-induced acute lung inflammation. J. Immunol. 2001, 166, 2071. [Google Scholar] [CrossRef] [Green Version]
  44. Dumont-Wallon, G.; Moyse, D.; Blouin, E.; Dreno, B. Bacterial resistance in French acne patients. Int. J. Dermatol. 2010, 49, 283–288. [Google Scholar] [CrossRef] [PubMed]
  45. Leyden, J.; Levy, S. The development of antibiotic resistance in Propionibacterium acnes. Cutis 2001, 67, 21–24. [Google Scholar] [PubMed]
  46. Smieja, M. Current indications for the use of clindamycin: A critical review. Can. J. Infect. Dis. Med. Microbiol. 1998, 9, 22–28. [Google Scholar] [CrossRef] [Green Version]
  47. Dréno, B.; Pécastaings, S.; Corvec, S.; Veraldi, S.; Khammari, A.; Roques, C. Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: A brief look at the latest updates. J. Eur. Acad. Dermatol. Venereol. 2018, 32, 5–14. [Google Scholar] [CrossRef] [Green Version]
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Figure 1. PEG-8 Laurate as a carbon source for fermentation of S. epidermidis, but not C. acnes. (a,b) S. epidermidis (S. epi) or (c,d) C. acnes (105 CFU/mL) was incubated with and without 2% PEG-8 Laurate in rich media (M) for 12 h. Rich media plus PEG-8 Laurate alone or bacteria alone served as controls. Bacterial fermentation was indicated by the color change of phenol red to yellow (arrow) and quantified by measurement of OD560 (b,d). Results were illustrated as the mean ± standard deviation (SD) of three independent experiments. *** p < 0.001 (two-tailed t-tests); ns = non-significant.
Figure 1. PEG-8 Laurate as a carbon source for fermentation of S. epidermidis, but not C. acnes. (a,b) S. epidermidis (S. epi) or (c,d) C. acnes (105 CFU/mL) was incubated with and without 2% PEG-8 Laurate in rich media (M) for 12 h. Rich media plus PEG-8 Laurate alone or bacteria alone served as controls. Bacterial fermentation was indicated by the color change of phenol red to yellow (arrow) and quantified by measurement of OD560 (b,d). Results were illustrated as the mean ± standard deviation (SD) of three independent experiments. *** p < 0.001 (two-tailed t-tests); ns = non-significant.
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Figure 2. Inhibition of C. acnes growth and MIP-2 production by PEG-8 Laurate fermentation of S. epidermidis in vivo. The ears of ICR mice were intradermally injected with C. acnes (107 CFU) and S. epidermidis (S. epi), (107 CFU) with 2% PEG-8 Laurate or H2O. Ear morphology 5 d after injection (a) and changes in ear thickness (mm) (b) were shown. Scale bar = 1 mm. (c) The CFUs (Log10 CFU/mL) were assessed by enumerating plating serial dilution (1:100–1:105) of the ear homogenates on agar plates. (d) The levels of MIP-2 cytokines in ear homogenates were measured by ELISA. Data are represented as mean ± SD of three separate experiments, using three mice per group. *** p < 0.001 (two-tailed t-tests).
Figure 2. Inhibition of C. acnes growth and MIP-2 production by PEG-8 Laurate fermentation of S. epidermidis in vivo. The ears of ICR mice were intradermally injected with C. acnes (107 CFU) and S. epidermidis (S. epi), (107 CFU) with 2% PEG-8 Laurate or H2O. Ear morphology 5 d after injection (a) and changes in ear thickness (mm) (b) were shown. Scale bar = 1 mm. (c) The CFUs (Log10 CFU/mL) were assessed by enumerating plating serial dilution (1:100–1:105) of the ear homogenates on agar plates. (d) The levels of MIP-2 cytokines in ear homogenates were measured by ELISA. Data are represented as mean ± SD of three separate experiments, using three mice per group. *** p < 0.001 (two-tailed t-tests).
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Figure 3. Reduction of the anti-C. acnes dose of clindamycin in the presence of fermentation media. The ears of ICR mice were intradermally injected with C. acnes (107 CFU), plus 0.1 or 1% of clindamycin (CDM) or H2O, along with media collected from culture of S. epidermidis (S. epi), with or without 2% PEG-8 Laurate. (a) Ears thickness (mm) was measured for 6 days after injection. (b) The CFUs were counted after plating serial dilution (1:100–1:105) of ear homogenates agar plates. (c) The levels of MIP-2 cytokines in ear homogenates were quantified by ELISA. Data are represented as mean ± SD of three independent experiments using three mice per group. ** p < 0.05; *** p < 0.001 (two-tailed t-tests); ns = non-significant.
Figure 3. Reduction of the anti-C. acnes dose of clindamycin in the presence of fermentation media. The ears of ICR mice were intradermally injected with C. acnes (107 CFU), plus 0.1 or 1% of clindamycin (CDM) or H2O, along with media collected from culture of S. epidermidis (S. epi), with or without 2% PEG-8 Laurate. (a) Ears thickness (mm) was measured for 6 days after injection. (b) The CFUs were counted after plating serial dilution (1:100–1:105) of ear homogenates agar plates. (c) The levels of MIP-2 cytokines in ear homogenates were quantified by ELISA. Data are represented as mean ± SD of three independent experiments using three mice per group. ** p < 0.05; *** p < 0.001 (two-tailed t-tests); ns = non-significant.
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Figure 4. Enhancement of anti-C. acnes activity and reduction of required doses of clindamycin by PEG-8 fermentation of S. epidermidis in an overlay assay. (a) C. acnes (106 CFU) in the presence or absence of 1 or 0.1% clindamycin (CDM) was overlaid on top of a lawn of S. epidermidis (108 CFU), with or without 2% PEG-8 Laurate. Scale bar = 1 cm (b) Graphical presentation of quantification of area (arbitrary unit, a.u.) of a C. acnes colony. Results display as mean ± SD in triplicate. ** p < 0.05 (two-tailed t-tests); ns = non-significant.
Figure 4. Enhancement of anti-C. acnes activity and reduction of required doses of clindamycin by PEG-8 fermentation of S. epidermidis in an overlay assay. (a) C. acnes (106 CFU) in the presence or absence of 1 or 0.1% clindamycin (CDM) was overlaid on top of a lawn of S. epidermidis (108 CFU), with or without 2% PEG-8 Laurate. Scale bar = 1 cm (b) Graphical presentation of quantification of area (arbitrary unit, a.u.) of a C. acnes colony. Results display as mean ± SD in triplicate. ** p < 0.05 (two-tailed t-tests); ns = non-significant.
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Marito, S.; Keshari, S.; Huang, C.-M. PEG-8 Laurate Fermentation of Staphylococcus epidermidis Reduces the Required Dose of Clindamycin Against Cutibacterium acnes. Int. J. Mol. Sci. 2020, 21, 5103. https://doi.org/10.3390/ijms21145103

AMA Style

Marito S, Keshari S, Huang C-M. PEG-8 Laurate Fermentation of Staphylococcus epidermidis Reduces the Required Dose of Clindamycin Against Cutibacterium acnes. International Journal of Molecular Sciences. 2020; 21(14):5103. https://doi.org/10.3390/ijms21145103

Chicago/Turabian Style

Marito, Shinta, Sunita Keshari, and Chun-Ming Huang. 2020. "PEG-8 Laurate Fermentation of Staphylococcus epidermidis Reduces the Required Dose of Clindamycin Against Cutibacterium acnes" International Journal of Molecular Sciences 21, no. 14: 5103. https://doi.org/10.3390/ijms21145103

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