Toxic Metals (As, Cd, Ni, Pb) Impact in the Most Common Medicinal Plant (Mentha piperita)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plants and Soil Characteristics
2.2. Experiment Characteristics
2.3. Sampling of Soil and Chemical Analyses
2.4. Sampling of Plants and Metal Analyses
2.5. Biometrical Measurements and Chlorophyll Detection
2.6. Data Analyses
2.7. Statistical Analysis
3. Results
3.1. Physical and Chemical Properties of Substrate and Watering Media
3.2. Metals Concentration in Soil Samples
3.3. Metals Concentration in Mint Samples
3.4. Biometrical Measurements and Chlorophyll Detection
3.5. Transfer Coefficient (TC) and Translocation Factor (TF)
4. Discussion
4.1. Metals Concentration in Mint Samples
4.2. Biometrical Measurements and Chlorophyll Detection
4.3. Transfer Coefficient (TC) and Translocation Factor (TF)
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Metals | Control Soil (M) ± SD (mg/kg d.w.) | Reference Values for Soils with Sensitive Uses (mg/kg d.w.) * | ||
---|---|---|---|---|
Normal Value | Alert Threshold | Intervention Threshold | ||
As | 1.29 ± 0.33 | 5 | 15 | 25 |
Cd | <0.08 ** | 1 | 3 | 5 |
Ni | 13.4 ± 0.12 | 20 | 75 | 150 |
Pb | 4.8 ± 0.56 | 20 | 50 | 100 |
Metals | LOD, mg/kg | Precision, % | Uncertainty, % | BCR-482 | ||
---|---|---|---|---|---|---|
Certified Value ± Uncertainty, mg/kg | Determined Value ± Uncertainty, mg/kg | Recovery, % | ||||
As | 0.25 | 7.50 | 15.3 | 0.85 ± 0.07 | 0.88 ± 0.13 | 103.5 |
Cd | 0.02 | 5.65 | 12.5 | 0.56 ± 0.02 | 0.54 ± 0.07 | 96.43 |
Cr | 0.02 | 4.55 | 13.1 | 4.12 ± 0.15 | 4.23 ± 0.55 | 102.7 |
Cu | 0.02 | 5.30 | 12.6 | 7.03 ± 0.19 | 6.94 ± 0.87 | 98.72 |
Ni | 0.04 | 4.80 | 12.7 | 2.47 ± 0.07 | 2.56 ± 0.33 | 103.7 |
Pb | 0.50 | 5.70 | 14.6 | 40.9 ± 1.4 | 39.6 ± 5.8 | 96.82 |
Zn | 0.03 | 5.35 | 12.7 | 100.6 ± 2.2 | 96.4 ± 12.2 | 95.81 |
Plant Organ System | Experiment | p Values | ||
---|---|---|---|---|
Cd | Ni | Pb | ||
Root–stem | M | ** (0.002) | ** (0.008) | ns (0.135) |
AsCd | ** (0.0001) | * (0.014) | * (0.043) | |
AsCdNiPb | * (0.022) | ** (0.003) | ns (0.110) | |
Root–leaves | M | ns (0.062) | * (0.018) | ns (0.256) |
AsCd | ** (0.0001) | ns (0.255) | * (0.036) | |
AsCdNiPb | * (0.010) | ** (0.002) | * (0.038) | |
Stem–leaves | M | ns (0.445) | ns (0.276) | ns (0.280) |
AsCd | ns (0.303) | ns (0.159) | ns (0.186) | |
AsCdNiPb | ns (0.169) | ns (0.306) | * (0.030) |
Plant Organ/Experiment | p Values | ||
---|---|---|---|
Cd | Ni | Pb | |
Root | |||
M/AsCd | ** (0.001) | ns (0.327) | ns (0.404) |
M/AsCdNiPb | ** (0.009) | ** (0.0005) | * (0.048) |
AsCd/AsCdNiPb | ns (0.234) | ** (0.005) | ns (0.074) |
Stem | |||
M/AsCd | ns (0.191) | ns (0.381) | ns (0.179) |
M/AsCdNiPb | * (0.030) | * (0.012) | * (0.040) |
AsCd/AsCdNiPb | * (0.049) | ** (0.009) | * (0.040) |
Leaves | |||
M/AsCd | ns (0.491) | ns (0.197) | ns (0.103) |
M/AsCdNiPb | ns (0.247) | ns (0.089) | ns (0.188) |
AsCd/AsCdNiPb | ns (0.220) | ns (0.171) | ns (0.095) |
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Dinu, C.; Gheorghe, S.; Tenea, A.G.; Stoica, C.; Vasile, G.-G.; Popescu, R.L.; Serban, E.A.; Pascu, L.F. Toxic Metals (As, Cd, Ni, Pb) Impact in the Most Common Medicinal Plant (Mentha piperita). Int. J. Environ. Res. Public Health 2021, 18, 3904. https://doi.org/10.3390/ijerph18083904
Dinu C, Gheorghe S, Tenea AG, Stoica C, Vasile G-G, Popescu RL, Serban EA, Pascu LF. Toxic Metals (As, Cd, Ni, Pb) Impact in the Most Common Medicinal Plant (Mentha piperita). International Journal of Environmental Research and Public Health. 2021; 18(8):3904. https://doi.org/10.3390/ijerph18083904
Chicago/Turabian StyleDinu, Cristina, Stefania Gheorghe, Anda Gabriela Tenea, Catalina Stoica, Gabriela-Geanina Vasile, Roxana Luisa Popescu, Ecaterina Anca Serban, and Luoana Florentina Pascu. 2021. "Toxic Metals (As, Cd, Ni, Pb) Impact in the Most Common Medicinal Plant (Mentha piperita)" International Journal of Environmental Research and Public Health 18, no. 8: 3904. https://doi.org/10.3390/ijerph18083904