The Antifungal Activity of Cinnamon-Litsea Combined Essential Oil against Dominant Fungal Strains of Moldy Peanut Kernels
Abstract
:1. Introduction
2. Results
2.1. Dominant Fungal Strain from Moldy Peanut Kernels
2.2. Antifungal Activity of Five Single EOs against Strain YQM
2.3. Antifungal Activity of Combined EOs against Strain YQM
2.4. Minimum Inhibitory Concentration (MIC) Value of CLCEO against Strain YQM
2.5. Component of CLCEO with Strongest Antifungal Effect against Strain YQM
2.6. Chemical Composition of EOs
2.7. Effect of EOs on the Microstructure of Strain YQM Mycelia
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Isolation and Identification of the Dominant Fungal Strain from Moldy Peanut Kernels
4.3. Determination of the Antifungal Activity of EOs against Strain YQM
4.4. Determination of MIC Value of CLCEO against Strain YQM
4.5. Determination of the Component of CLCEO with Strongest Antifungal Effect against Strain YQM
4.6. Determination of the Chemical Composition of EOs
4.7. Determination of the Effect of EOs on the Microstructure of Strain YQM Mycelia
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Li, Y.; Zhang, R.; Qin, X.; Liao, Y.; Siddique, K.H.M.; Prasad, D.M. Changes in the protein and fat contents of peanut (Arachis hypogaea L.) cultivars released in China in the last 60 years. Plant Breed. 2018, 137, 746–756. [Google Scholar] [CrossRef]
- Lavrinenko, I.A.; Donskikh, A.O.; Minakov, D.A.; Sirota, A.A. Analysis and classification of peanuts with fungal diseases based on real-time spectral processing. Food Addit. Contam. A 2022, 39, 1–11. [Google Scholar] [CrossRef] [PubMed]
- Juhaimi, F.A.; Ghafoor, K.; Babiker, E.E.; Ozcan, M.M.; Aadiamo, O.Q.; Alsawmahi, O.N. Influence of storage and roasting on the quality properties of kernel and oils of raw and roasted peanuts. J. Oleo. Sci. 2018, 67, 755–762. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Groot, S.P.C.; van Litsenburg, M.J.; Kodde, J.; Hall, R.D.; de Vos, R.C.H.; Mumm, R. Analyses of metabolic activity in peanuts under hermetic storage at different relative humidity levels. Food Chem. 2022, 373, 131020. [Google Scholar] [CrossRef]
- Norlia, M.; Jinap, S.; Nor-Khaizura, M.A.R.; Son, R.; Chin, C.K. Sardjono Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia. Int. J. Food Microbiol. 2018, 282, 9–15. [Google Scholar] [CrossRef]
- Yang, B.; Geng, H.; Nie, C.; Wang, G.; Xiang, F.; Zhang, C.; Liu, Y. The relationship between Aspergillus flavus in peanut soil and aflatoxin contamination of peanut in China. J. Nucl. Agric. Sci. 2021, 35, 863–869. (In Chinese) [Google Scholar]
- Bryson, R. Evaluating the contribution of synthetic fungicides to cereal plant health and CO2 reduction targets against the backdrop of the increasingly complex regulatory environment in Europe. Plant Pathol. 2021, 71, 170–186. [Google Scholar] [CrossRef]
- El-Baky, N.A.; Amara, A. Recent approaches towards control of fungal diseases in plants: An Updated Review. J. Fungi 2021, 7, 900. [Google Scholar] [CrossRef]
- Spengler, G.; Gajdacs, M.; Donadu, M.G.; Usai, M.; Marchetti, M.; Ferrari, M.; Mazzarello, V.; Zanetti, S.; Nagy, F.; Kovacs, R. Evaluation of the antimicrobial and antivirulent potential of essential oils isolated from Juniperus oxycedrus L. ssp. macrocarpa aerial parts. Microorganisms 2022, 10, 758. [Google Scholar] [CrossRef]
- Matrose, N.A.; Obikeze, K.; Belay, Z.A.; Caleb, O.J. Plant extracts and other natural compounds as alternatives for post-harvest management of fruit fungal pathogens: A review. Food Biosci. 2021, 41, 100840. [Google Scholar] [CrossRef]
- Sellitto, V.M.; Zara, S.; Fracchetti, F.; Capozzi, V.; Nardi, T. Microbial biocontrol as an alternative to synthetic fungicides: Boundaries between pre- and postharvest applications on vegetables and fruits. Fermentation 2021, 7, 60. [Google Scholar] [CrossRef]
- Brito, V.D.; Achimón, F.; Dambolena, J.S.; Pizzolitto, R.P.; Zygadlo, J.A. Trans-2-hexen-1-ol as a tool for the control of Fusarium verticillioides in stored maize grains. J. Stored Prod. Res. 2019, 82, 123–130. [Google Scholar] [CrossRef]
- Lin, H.-J.; Lin, Y.-L.; Huang, B.-B.; Lin, Y.-T.; Li, H.-K.; Lu, W.-J.; Lin, T.-C.; Tsui, Y.-C.; Lin, H.-T.V. Solid- and vapour-phase antifungal activities of six essential oils and their applications in postharvest fungal control of peach (Prunus persica L. Batsch). LWT-Food Sci. Technol. 2022, 156, 113031. [Google Scholar] [CrossRef]
- Reyes-Jurado, F.; Barcena-Massberg, Z.; Ramirez-Corona, N.; Lopez-Malo, A.; Palou, E. Fungal inactivation on Mexican corn tortillas by means of thyme essential oil in vapor-phase. Curr. Res. Food Sci. 2022, 5, 629–633. [Google Scholar] [CrossRef]
- Strelkova, T.; Nemes, B.; Kovacs, A.; Novotny, D.; Bozik, M.; Kloucek, P. Inhibition of fungal strains isolated from cereal grains via vapor phase of essential oils. Molecules 2021, 26, 1313. [Google Scholar] [CrossRef]
- Ju, J.; Xu, X.; Xie, Y.; Guo, Y.; Cheng, Y.; Qian, H.; Yao, W. Inhibitory effects of cinnamon and clove essential oils on mold growth on baked foods. Food Chem. 2018, 240, 850–855. [Google Scholar] [CrossRef]
- Anžlovar, S.; Likar, M.; Koce, J.D. Antifungal potential of thyme essential oil as a preservative for storage of wheat seeds. Acta Bot. Croat. 2017, 76, 64–71. [Google Scholar] [CrossRef] [Green Version]
- Raveau, R.; Fontaine, J.; Soltani, A.; Mediouni Ben Jemaa, J.; Laruelle, F.; Lounes-Hadj Sahraoui, A. In vitro potential of clary sage and coriander essential oils as crop protection and post-harvest decay control products. Foods 2022, 11, 312. [Google Scholar] [CrossRef]
- Ju, J.; Xie, Y.; Yu, H.; Guo, Y.; Cheng, Y.; Qian, H.; Yao, W. Analysis of the synergistic antifungal mechanism of eugenol and citral. LWT-Food Sci. Technol. 2020, 123, 109128. [Google Scholar] [CrossRef]
- Purkait, S.; Bhattacharya, A.; Bag, A.; Chattopadhyay, R.R. Synergistic antibacterial, antifungal and antioxidant efficacy of cinnamon and clove essential oils in combination. Arch. Microbiol. 2020, 202, 1439–1448. [Google Scholar] [CrossRef]
- Yuan, W.; Teo, C.H.M.; Yuk, H.-G. Combined antibacterial activities of essential oil compounds against Escherichia coli O157:H7 and their application potential on fresh-cut lettuce. Food Control. 2019, 96, 112–118. [Google Scholar] [CrossRef]
- Hlebová, M.; Lukas Hleba, J.M.; Kováčik, A.; Čuboň, J.; Ivana, C.; Uzsáková, V.; Božik, M.; Klouček, P. Antifungal and synergistic activities of some selected essential oils on the growth of significant indoor fungi of the genus Aspergillus. J. Environ. Sci. Health A Tox. Hazard. Subst. Environ. Eng. 2021, 56, 1335–1346. [Google Scholar] [CrossRef] [PubMed]
- Fierascu, R.C.; Fierascu, I.C.; Dinu-Pirvu, C.E.; Fierascu, I.; Paunescu, A. The application of essential oils as a nextgeneration of pesticides: Recent developments and future perspectives. Z. Naturforsch. 2020, 75, 183–204. [Google Scholar] [CrossRef] [PubMed]
- Mutlu-Ingok, A.; Devecioglu, D.; Dikmetas, D.N.; Karbancioglu-Guler, F.; Capanoglu, E. Antibacterial, antifungal, antimycotoxigenic, and antioxidant activities of essential oils: An Updated Review. Molecules 2020, 25, 4711. [Google Scholar] [CrossRef] [PubMed]
- Xia, S.; Lin, H.; Zhu, P.; Wang, P.; Liao, S.; Chen, S.; Wang, Z.; Fan, G.; Rengasamy, K.R.R. Inhibitory effects of Litsea cubeba oil and Its active components on Aspergillus flavus. J. Food Qual. 2020, 2020, 1–9. [Google Scholar] [CrossRef]
- Xiang, F.; Zhao, Q.; Zhao, K.; Pei, H.; Tao, F. The efficacy of composite essential oils against aflatoxigenic fungus Aspergillus flavus in maize. Toxins 2020, 12, 562–578. [Google Scholar] [CrossRef] [PubMed]
- Huang, Y.; Kuang, Z.; Deng, Z.; Zhang, R.; Cao, L. Endophytic bacterial and fungal communities transmitted from cotyledons and germs in peanut (Arachis hypogaea L.) sprouts. Environ. Sci. Pollut. Res. 2017, 24, 16458–16464. [Google Scholar] [CrossRef]
- Mamo, F.T.; Shang, B.; Selvaraj, J.N.; Wang, Y.; Liu, Y. Isolation and characterization of Aspergillus flavus strains in China. J. Microbiol. 2018, 56, 119–127. [Google Scholar] [CrossRef]
- Norlia, M.; Jinap, S.; Nor-Khaizura, M.A.R.; Radu, S.; Samsudin, N.I.P.; Azri, F.A. Aspergillus section Flavi and aflatoxins: Occurrence, detection, and identification in raw peanuts and peanut-based products along the supply chain. Front. Microbiol. 2019, 10, 2602. [Google Scholar] [CrossRef] [Green Version]
- Mou, L.; Du, X.; Lu, X.; Lu, Y.; Li, G.; Li, J. Component analysis and antifungal activity of three Chinese herbal essential oils and their application of postharvest preservation of peach fruit. LWT-Food Sci. Technol. 2021, 151, 112089. [Google Scholar] [CrossRef]
- Denkova-Kostova, R.; Teneva, D.; Tomova, T.; Goranov, B.; Denkova, Z.; Shopska, V.; Slavchev, A.; Hristova-Ivanova, Y. Chemical composition, antioxidant and antimicrobial activity of essential oils from tangerine (Citrus reticulata L.), grapefruit (Citrus paradisi L.), lemon (Citrus lemon L.) and cinnamon (Cinnamomum zeylanicum Blume). Z. Naturforsch. 2021, 76, 175–185. [Google Scholar] [CrossRef] [PubMed]
- Sun, Q.; Li, J.; Sun, Y.; Chen, Q.; Zhang, L.; Le, T. The antifungal effects of cinnamaldehyde against Aspergillus niger and its application in bread preservation. Food Chem. 2020, 317, 126405. [Google Scholar] [CrossRef] [PubMed]
- Wei, J.; Bi, Y.; Xue, H.; Wang, Y.; Zong, Y.; Prusky, D. Antifungal activity of cinnamaldehyde against Fusarium sambucinum involves inhibition of ergosterol biosynthesis. J. Appl. Microbiol. 2020, 129, 256–265. [Google Scholar] [CrossRef]
- Achar, P.N.; Quyen, P.; Adukwu, E.C.; Sharma, A.; Msimanga, H.Z.; Nagaraja, H.; Sreenivasa, M.Y. Investigation of the antifungal and anti-aflatoxigenic potential of plant-based essential oils against Aspergillus flavus in peanuts. J. Fungi 2020, 6, 383–402. [Google Scholar] [CrossRef] [PubMed]
- Arasu, M.V.; Viayaraghavan, P.; Ilavenil, S.; Al-Dhabi, N.A.; Choi, K.C. Essential oil of four medicinal plants and protective properties in plum fruits against the spoilage bacteria and fungi. Ind. Crop. Prod. 2019, 133, 54–62. [Google Scholar] [CrossRef]
- Sawadogo, I.; Pare, A.; Kabore, D.; Montet, D.; Durand, N.; Bouajila, J.; Zida, E.P.; Sawadogo-Lingani, H.; Nikiema, P.A.; Nebie, R.H.C.; et al. Antifungal and antiaflatoxinogenic effects of Cymbopogon citratus, Cymbopogon nardus, and Cymbopogon schoenanthus essential oils alone and in combination. J. Fungi 2022, 8, 117. [Google Scholar] [CrossRef]
- Yan, J.; Niu, Y.; Wu, C.; Shi, Z.; Zhao, P.; Naik, N.; Mai, X.; Yuan, B. Antifungal effect of seven essential oils on bamboo. Adv. Compos. Hybrid. Ma. 2021, 4, 552–561. [Google Scholar] [CrossRef]
- Hassan, H.A.; Genaidy, M.M.; Kamel, M.S.; Abdelwahab, S.F. Synergistic antifungal activity of mixtures of clove, cumin and caraway essential oils and their major active components. J. Herb. Med. 2020, 24, 100399. [Google Scholar] [CrossRef]
- Nikkhah, M.; Hashemi, M.; Habibi Najafi, M.B.; Farhoosh, R. Synergistic effects of some essential oils against fungal spoilage on pear fruit. Int. J. Food Microbiol. 2017, 257, 285–294. [Google Scholar] [CrossRef]
- Aimad, A.; Youness, E.A.; Sanae, R.; El Moussaoui, A.; Bourhia, M.; Salamatullah, A.M.; Alzahrani, A.; Alyahya, H.K.; Albadr, N.A.; Nafidi, H.A.; et al. Chemical composition and antifungal, insecticidal and repellent activity of essential oils from Origanum compactum Benth. used in the mediterranean diet. Front. Plant Sci. 2022, 13, 798259. [Google Scholar] [CrossRef]
- Dassanayake, M.K.; Chong, C.H.; Khoo, T.J.; Figiel, A.; Szumny, A.; Choo, C.M. Synergistic field crop pest management properties of plant-derived essential oils in combination with synthetic pesticides and bioactive molecules: A Review. Foods 2021, 10, 2016. [Google Scholar] [CrossRef] [PubMed]
- Gadban, L.C.; Camiletti, B.X.; Bigatton, E.D.; Distéfano, S.G.; Lucini, E.I. Combinations of Tagetes filifolia Lag. essential oil with chemical fungicides to control Colletotrichum truncatum and their effects on the biocontrol agent Trichoderma harzianum. J. Plant Prot. Res. 2020, 60, 41–50. [Google Scholar]
- Glamočlija, J.; Soković, M.; Tešević, V.; Linde, G.A.; Colauto, N.B. Chemical characterization of Lippia alba essential oil: An alternative to control green molds. Braz. J. Microbiol. 2011, 42, 1537–1546. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Altun, M.; Yapici, B.M. Determination of chemical compositions and antibacterial effects of selected essential oils against human pathogenic strains. An. Acad. Bras. Cienc. 2022, 94, e20210074. [Google Scholar] [CrossRef] [PubMed]
- Hlebova, M.; Hleba, L.; Medo, J.; Uzsakova, V.; Kloucek, P.; Bozik, M.; Hascik, P.; Cubon, J. Antifungal and antitoxigenic effects of selected essential oils in vapors on green coffee beans with impact on consumer acceptability. Foods 2021, 10, 2993–3011. [Google Scholar] [CrossRef]
- Zhao, J.; Wang, Q.; Ma, J. Chemical composition and anti-arthritic activity of the essential oil from Litsea cubeba against Type II collagen rheumatoid arthritis in rat collagen. Trop. J. Pharm. Res. 2020, 19, 645–650. [Google Scholar] [CrossRef]
- Liang, Y.; Li, Y.; Sun, A.; Liu, X. Chemical compound identification and antibacterial activity evaluation of cinnamon extracts obtained by subcritical n-butane and ethanol extraction. Food Sci. Nutr. 2019, 7, 2186–2193. [Google Scholar] [CrossRef] [Green Version]
- Correa, A.N.R.; Ferreira, C.D. Essential oil for the control of fungi, bacteria, yeasts and viruses in food: An overview. Crit. Rev. Food Sci. Nutr. 2022, 62, 1–15. [Google Scholar] [CrossRef]
- Tang, X.; Shao, Y.L.; Tang, Y.J.; Zhou, W.W. Antifungal activity of essential oil compounds (geraniol and citral) and inhibitory mechanisms on grain pathogens (Aspergillus flavus and Aspergillus ochraceus). Molecules 2018, 23, 2108. [Google Scholar] [CrossRef] [Green Version]
- Oliveira, R.C.; Carvajal-Moreno, M.; Correa, B.; Rojo-Callejas, F. Cellular, physiological and molecular approaches to investigate the antifungal and anti-aflatoxigenic effects of thyme essential oil on Aspergillus flavus. Food Chem. 2020, 315, 126096. [Google Scholar] [CrossRef]
- Wang, D.; Zhang, J.; Jia, X.; Xin, L.; Zhai, H. Antifungal effects and potential mechanism of essential oils on collelotrichum gloeosporioides in vitro and in vivo. Molecules 2019, 24, 3386–3398. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Sarathambal, C.; Rajagopal, S.; Viswanathan, R. Mechanism of antioxidant and antifungal properties of Pimenta dioica (L.) leaf essential oil on Aspergillus flavus. J. Food Sci. Technol. 2020, 58, 2497–2506. [Google Scholar] [CrossRef] [PubMed]
- Brandão, R.M.; Ferreira, V.R.F.; Batista, L.R.; Alves, E.; Lira, N.d.A.; Bellete, B.S.; Scolforo, J.R.S.; Cardoso, M.d.G. Antifungal and antimycotoxigenic effect of the essential oil of Eremanthus erythropappus on three different Aspergillus species. Flavour Fragr. J. 2020, 35, 524–533. [Google Scholar] [CrossRef]
- Cai, J.; Yan, R.; Shi, J.; Chen, J.; Long, M.; Wu, W.; Kuca, K. Antifungal and mycotoxin detoxification ability of essential oils: A review. Phytother. Res. 2021, 36, 1–11. [Google Scholar] [CrossRef] [PubMed]
- Sharma, R.; Rao, R.; Kumar, S.; Mahant, S.; Khatkar, S. Therapeutic Potential of Citronella Essential Oil: A Review. Curr. Drug Discov. Technol. 2019, 16, 330–339. [Google Scholar] [CrossRef]
- El Euony, O.I.; Elblehi, S.S.; Abdel-Latif, H.M.; Abdel-Daim, M.M.; El-Sayed, Y.S. Modulatory role of dietary Thymus vulgaris essential oil and Bacillus subtilis against thiamethoxam-induced hepatorenal damage, oxidative stress, and immunotoxicity in African catfish (Clarias garipenus). Environ. Sci. Pollut. Res. 2020, 27, 23108–23128. [Google Scholar] [CrossRef]
- Additives, E.P.O.; Products or Substances used in Animal, F.; Bampidis, V.; Azimonti, G.; Bastos, M.L.; Christensen, H.; Fasmon Durjava, M.; Kouba, M.; Lopez-Alonso, M.; Lopez Puente, S.; et al. Safety and efficacy of a feed additive consisting of an essential oil from the fruits of Litsea cubeba (Lour.) Pers. (litsea berry oil) for use in all animal species (FEFANA asbl). EFSA J. 2021, 19, 6623. [Google Scholar]
- Khorram, F.; Ramezanian, A. Cinnamon essential oil incorporated in shellac, a novel bio-product to maintain quality of ‘Thomson navel’ orange fruit. J. Food Sci. Technol. 2021, 58, 2963–2972. [Google Scholar] [CrossRef]
- Sarengaowa; Hu, W.; Jiang, A.; Xiu, Z.; Feng, K. Effect of thyme oil-alginate based coating on quality and microbial safety of fresh-cut apples. J. Sci. Food Agric. 2018, 98, 2302–2311. [Google Scholar] [CrossRef]
- Jia, B.; Xu, L.; Guan, W.; Lin, Q.; Brennan, C.; Yan, R.; Zhao, H. Effect of citronella essential oil fumigation on sprout suppression and quality of potato tubers during storage. Food Chem. 2019, 284, 254–258. [Google Scholar] [CrossRef] [PubMed]
- Kumar, S.; Stecher, G.; Li, M.; Knyaz, C.; Tamura, K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol. Biol. Evol. 2018, 35, 1547–1549. [Google Scholar] [CrossRef] [PubMed]
- Ma, W.; Zhao, L.; Zhao, W.; Xie, Y. (E)-2-hexenal, as a potential natural antifungal compound, inhibits Aspergillus flavus spore germination by disrupting mitochondrial energy metabolism. J. Agric. Food Chem. 2019, 67, 1138–1145. [Google Scholar] [CrossRef] [PubMed]
- Zhang, Z.; Qin, Q.; Ding, R.; Xia, Y.; Xiong, L.; Bi, Y.; Prusky, D. Acidolysis-dominated pretreatment elevates distillation yield and impacts composition, antioxidant and antifungal activities of essential oil from Cuminum cyminum seeds. RSC Adv. 2018, 8, 32283–32295. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Wang, D.; Meng, Y.; Zhao, X.; Fan, W.; Yi, T.; Wang, X. Sunflower oil flavored by essential oil from Punica granatum cv. Heyinshiliu peels improved its oxidative stability and sensory properties. LWT-Food Sci. Technol. 2019, 111, 55–61. [Google Scholar] [CrossRef]
EOs | Different Concentrations of EOs | MIC | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
2 | 1 | 0.5 | 0.25 | 0.125 | 0.0625 | 0.0313 | 0.0156 | Blank | (µL/mL) | |
CEO | — | − | − | − | − | − | + | + | + | 0.0625 |
LEO | − | − | — | w | w | + | + | + | + | 0.5 |
CLCEO | − | − | − | − | − | − | − | + | + | 0.0313 |
Compounds | Retention Time/min | CEO/% | LEO/% | CLCEO/% |
---|---|---|---|---|
2-Methylheptane | 5.517 | 0.31 | — | − |
n-Octane | 6.181 | 1.72 | − | − |
2,4-Dimethyl-heptane | 6.697 | 1.43 | − | − |
4-Methyloctane | 7.846 | 0.19 | − | − |
Cyclofenchene | 10.065 | 0.18 | − | − |
Pinene | 10.537 | 1.53 | 4.47 | 0.59 |
Camphene | 11.215 | 1.04 | 1.07 | 0.10 |
Benzaldehyde | 11.945 | 17.20 | − | − |
Sabinene | 12.260 | − | 9.43 | 0.46 |
trans-pinocarveol | 12.432 | − | 3.90 | − |
Myrcene | 13.028 | − | 2.96 | 0.21 |
Decane | 13.437 | 1.29 | − | − |
α-Terpinene | 14.423 | − | 0.13 | − |
4-Methyldecane | 14.594 | 0.31 | − | − |
Cineole | 15.254 | 23.48 | − | − |
limonene | 15.318 | − | 30.56 | 2.83 |
γ-Terpinene | 16.760 | − | 0.34 | − |
Sabinene hydrate | 17.542 | − | 0.37 | − |
Isogeranialdehyde | 17.75 | − | 3.87 | 0.76 |
Linalool | 19.492 | − | 3.13 | 0.21 |
Phenethyl alcohol | 20.844 | 4.75 | − | − |
7-methyl-3-methylene-6-octena | 21.974 | − | 1.79 | − |
Citronellal | 22.535 | 0.54 | 1.14 | 0.19 |
Dodecane | 25.287 | 3.76 | − | − |
α-Terpineol | 25.592 | − | 0.77 | − |
Cinnamaldehyde | 27.202 | 28.48 | − | 49.33 |
Citral | 28.266 | 0.35 | 31.27 | 34.77 |
1,3-Di-tert-butylbenzene | 28.849 | 0.99 | − | − |
2,4-Dimethyldodecane | 29.346 | 0.51 | − | − |
4,6-Dimethyldodecane | 30.303 | 1.01 | − | − |
γ-Elemene | 34.300 | − | 0.13 | − |
2-Methyl-3-phenyl-2-propenal | 34.605 | 0.98 | − | − |
α-Terpineyl Acetate | 35.247 | − | 0.22 | − |
Piperitene | 36.839 | − | 0.37 | − |
2,6-di-tert-butyl-4-methylphenol | 37.11 | − | − | 0.87 |
Caryophyllene | 39.453 | 3.52 | 2.53 | 0.56 |
Cinnamyl ester | 41.123 | 2.58 | − | − |
Eugenol | 47.633 | 3.55 | − | 9.11 |
Total | − | 99.71 | 98.42 | 99.99 |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Liu, Y.; Wang, R.; Zhao, L.; Huo, S.; Liu, S.; Zhang, H.; Tani, A.; Lv, H. The Antifungal Activity of Cinnamon-Litsea Combined Essential Oil against Dominant Fungal Strains of Moldy Peanut Kernels. Foods 2022, 11, 1586. https://doi.org/10.3390/foods11111586
Liu Y, Wang R, Zhao L, Huo S, Liu S, Zhang H, Tani A, Lv H. The Antifungal Activity of Cinnamon-Litsea Combined Essential Oil against Dominant Fungal Strains of Moldy Peanut Kernels. Foods. 2022; 11(11):1586. https://doi.org/10.3390/foods11111586
Chicago/Turabian StyleLiu, Yijun, Ruolan Wang, Lingli Zhao, Shanshan Huo, Shichang Liu, Hanxiao Zhang, Akio Tani, and Haoxin Lv. 2022. "The Antifungal Activity of Cinnamon-Litsea Combined Essential Oil against Dominant Fungal Strains of Moldy Peanut Kernels" Foods 11, no. 11: 1586. https://doi.org/10.3390/foods11111586