Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using Lactobacillus rhamnosus GG Bacteria
Abstract
:1. Introduction
2. Materials and Methods
2.1. General Instrumentation and Materials
2.2. Coating Glass with Gold Nanoparticles
2.3. Surface Inoculation with Lactobacillus rhamnosus GG Bacteria
2.4. Formation of the Final Coating
2.5. Microscopic Imaging of Surfaces Obtained at Various Stages of the Synthesis
2.5.1. Scanning Electron Microscopy (SEM)
2.5.2. Optical Microscopy and Counting Mammalian Cells
2.6. DLS and Zeta Potential Measurements
2.7. Evaluation of Bacterial Counts by Crystal Violet Staining
2.8. Study of Cell Cultures Growth on the New Coating
3. Results and Discussion
3.1. Formation of the Gold Nanoparticle Layer on Glass
3.2. Lactobacillus rhamnosus GG Bacteria on the Obtained Surface
3.3. Chemical Analysis and Imaging of the Final Coating
3.4. Aplication of Obtained Coatings for Cell Cultures
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kaminski, K.; Syrek, K.; Grudzień, J.; Obloza, M.; Adamczyk, M.; Sulka, G.D. Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using Lactobacillus rhamnosus GG Bacteria. Coatings 2021, 11, 1410. https://doi.org/10.3390/coatings11111410
Kaminski K, Syrek K, Grudzień J, Obloza M, Adamczyk M, Sulka GD. Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using Lactobacillus rhamnosus GG Bacteria. Coatings. 2021; 11(11):1410. https://doi.org/10.3390/coatings11111410
Chicago/Turabian StyleKaminski, Kamil, Karolina Syrek, Joanna Grudzień, Magdalena Obloza, Monika Adamczyk, and Grzegorz D. Sulka. 2021. "Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using Lactobacillus rhamnosus GG Bacteria" Coatings 11, no. 11: 1410. https://doi.org/10.3390/coatings11111410