Boosting the Intra-Articular Efficacy of Low Dose Corticosteroid through a Biopolymeric Matrix: An In Vivo Model of Osteoarthritis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Material Formulations
2.2. Experimental Design and OA Induction
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- Group 1: TA (Kenacort® 40 mg/mL).
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- Group 2: HY-CTL.
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- Group 3: RV2 (HY-CTL + 2.0 mg/mL TA).
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- Group 4: RV4.5 (HY-CTL + 4.5 mg/mL TA).
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- Group 5: NaCl (0.9% sterile saline solution).
2.3. Local Pain Sensitivity and Gait Measurements
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- Paw area: the total floor area contacted by the paw during the stance phase.
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- Stand: the duration in seconds of contact of a paw with the glass plate.
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- Swing: the duration in seconds of no contact of a paw with the glass plate.
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- Single stance: the duration in seconds of contact with the glass plate with only one paw.
2.4. Histology
2.5. Immunohistochemistry
2.6. Statistical Analysis
3. Results
3.1. Clinical Observations
3.2. Local Pain Sensitivity and Gait Measurements
3.3. Histology
3.4. Immunohistochemistry
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Tschon, M.; Salamanna, F.; Martini, L.; Giavaresi, G.; Lorenzini, L.; Calzà, L.; Fini, M. Boosting the Intra-Articular Efficacy of Low Dose Corticosteroid through a Biopolymeric Matrix: An In Vivo Model of Osteoarthritis. Cells 2020, 9, 1571. https://doi.org/10.3390/cells9071571
Tschon M, Salamanna F, Martini L, Giavaresi G, Lorenzini L, Calzà L, Fini M. Boosting the Intra-Articular Efficacy of Low Dose Corticosteroid through a Biopolymeric Matrix: An In Vivo Model of Osteoarthritis. Cells. 2020; 9(7):1571. https://doi.org/10.3390/cells9071571
Chicago/Turabian StyleTschon, Matilde, Francesca Salamanna, Lucia Martini, Gianluca Giavaresi, Luca Lorenzini, Laura Calzà, and Milena Fini. 2020. "Boosting the Intra-Articular Efficacy of Low Dose Corticosteroid through a Biopolymeric Matrix: An In Vivo Model of Osteoarthritis" Cells 9, no. 7: 1571. https://doi.org/10.3390/cells9071571