Mitochondrial Permeability Transition Causes Mitochondrial Reactive Oxygen Species- and Caspase 3-Dependent Atrophy of Single Adult Mouse Skeletal Muscle Fibers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Surgical Methods
2.2. Single Fiber Isolation and Culture
2.3. mROS Emission and Caspase-3 Activity
2.4. Acute Inhibition of MPT, mROS and Caspase-3 Activity
2.5. ‘Disuse’ Atrophy Experiments
2.6. Statistics
3. Results
3.1. Acute Myofiber Culture Experiments
3.2. ‘Disuse’ Muscle Atrophy Model Experiments
4. Discussion
4.1. mROS Emission and Caspase-3 Activation Increase Following MPT in Single Isolated Mouse Muscle Fibers, and Are Accompanied by a Decrease in Myofiber Diameter
4.2. MPT, mROS, and Caspase-3 in a Single Muscle Fiber Model of ‘Disuse’
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Skinner, S.K.; Solania, A.; Wolan, D.W.; Cohen, M.S.; Ryan, T.E.; Hepple, R.T. Mitochondrial Permeability Transition Causes Mitochondrial Reactive Oxygen Species- and Caspase 3-Dependent Atrophy of Single Adult Mouse Skeletal Muscle Fibers. Cells 2021, 10, 2586. https://doi.org/10.3390/cells10102586
Skinner SK, Solania A, Wolan DW, Cohen MS, Ryan TE, Hepple RT. Mitochondrial Permeability Transition Causes Mitochondrial Reactive Oxygen Species- and Caspase 3-Dependent Atrophy of Single Adult Mouse Skeletal Muscle Fibers. Cells. 2021; 10(10):2586. https://doi.org/10.3390/cells10102586
Chicago/Turabian StyleSkinner, Sarah K., Angelo Solania, Dennis W. Wolan, Michael S. Cohen, Terence E. Ryan, and Russell T. Hepple. 2021. "Mitochondrial Permeability Transition Causes Mitochondrial Reactive Oxygen Species- and Caspase 3-Dependent Atrophy of Single Adult Mouse Skeletal Muscle Fibers" Cells 10, no. 10: 2586. https://doi.org/10.3390/cells10102586