Carbonic Anhydrase IV Selective Inhibitors Counteract the Development of Colitis-Associated Visceral Pain in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemistry
2.2. Synthesis of 4-(2-(6-Amino-7H-purin-7-yl)ethoxy)benzenesulfonamide AB-118
2.3. Animals
2.4. Induction of Colitis and Drug Administration
2.5. Assessment of Visceral Sensitivity by Viscero-Motor Response (VMR)
2.6. Assessment of Visceral Sensitivity by Abdominal Withdrawal Reflex (AWR)
2.7. Histological Analysis of Colonic Damage
2.8. Kinetic Determination of CA IV Activity in Colon Samples and AB-118 Inhibition Profile
2.9. Immunohistochemical Analysis of CA IV Expression
2.10. Statistics
3. Results
3.1. The CA IV Inhibitors AB-118 and NIK-67 Counteracted the Development of Visceral Pain Induced by Colitis in Rats
3.2. Effect of the Repeated Administration of AB2-118 and NIK-67 on Colon Damage
3.3. Effect of the Repeated Administration of AB2-118 and NIK-67 on Intestinal CA IV Activity
3.4. The Expression of CA IV Resulted Increased through the Colon Wall of DNBS-Treated Rats
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lucarini, E.; Nocentini, A.; Bonardi, A.; Chiaramonte, N.; Parisio, C.; Micheli, L.; Toti, A.; Ferrara, V.; Carrino, D.; Pacini, A.; et al. Carbonic Anhydrase IV Selective Inhibitors Counteract the Development of Colitis-Associated Visceral Pain in Rats. Cells 2021, 10, 2540. https://doi.org/10.3390/cells10102540
Lucarini E, Nocentini A, Bonardi A, Chiaramonte N, Parisio C, Micheli L, Toti A, Ferrara V, Carrino D, Pacini A, et al. Carbonic Anhydrase IV Selective Inhibitors Counteract the Development of Colitis-Associated Visceral Pain in Rats. Cells. 2021; 10(10):2540. https://doi.org/10.3390/cells10102540
Chicago/Turabian StyleLucarini, Elena, Alessio Nocentini, Alessandro Bonardi, Niccolò Chiaramonte, Carmen Parisio, Laura Micheli, Alessandra Toti, Valentina Ferrara, Donatello Carrino, Alessandra Pacini, and et al. 2021. "Carbonic Anhydrase IV Selective Inhibitors Counteract the Development of Colitis-Associated Visceral Pain in Rats" Cells 10, no. 10: 2540. https://doi.org/10.3390/cells10102540