NF-κB Signaling Is Regulated by Fucosylation in Metastatic Breast Cancer Cells
Abstract
:1. Introduction
2. Experimental Section
2.1. Cell Culture
(Full Details for Reagents and Global Proteomics in Supplemental File S2)
2.2. Protein Analysis
2.3. RNA Analysis
2.4. Quantitative Global Proteomic Comparison of Protein Levels
3. Results
3.1. Pharmacological Inhibition of Fucosylation Alters N-Glycan Processing
3.2. NF-κB Activity Is Reduced in 2FF-Treated 4T1 Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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KEGG Pathways | Reactome Pathways | ||||
---|---|---|---|---|---|
Decreased | |||||
Description | Count in network | FDR | Description | Count in network | FDR |
NF-κB signaling pathway | 9 or 93 | 0.0002 | Vesicle-mediated transport | 23 of 553 | 1.39 × 10−5 |
RIG-1-like receptor signaling pathway | 6 of 68 | 0.0062 | Membrane trafficking | 22 of 523 | 1.39 × 10−5 |
Tight Junction | 9 of 165 | 0.0062 | ER to Golgi Anterograde Transport | 12 of 147 | 1.45 × 10−5 |
Protein processing in the ER | 9 of 161 | 0.0062 | Asparagine N-linked glycosylation | 15 of 269 | 3.02 × 10−5 |
TNF signaling pathway | 7 of 108 | 0.0068 | Immune system | 38 of 1523 | 4.78 × 10−5 |
Increased | |||||
Spliceosome | 16 of 130 | 1.73 × 10−11 | Metabolism of RNA | 33 of 448 | 4.42 × 10−18 |
Ribosome biogenesis in eukaryotes | 7 of 76 | 5 × 10−4 | Processing of Capped Intron-containing Pre-mRNA | 23 of 212 | 1.02 × 10−15 |
Huntington’s disease | 8 of 187 | 0.0134 | mRNA splicing – Major Pathway | 19 of 156 | 8.94 × 10−14 |
DNA replication | 4 of 35 | 0.0134 | Gene Expression (transcription) | 30 of 858 | 7.30 × 10−9 |
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Doud, E.H.; Shetty, T.; Abt, M.; Mosley, A.L.; Corson, T.W.; Mehta, A.; Yeh, E.S. NF-κB Signaling Is Regulated by Fucosylation in Metastatic Breast Cancer Cells. Biomedicines 2020, 8, 600. https://doi.org/10.3390/biomedicines8120600
Doud EH, Shetty T, Abt M, Mosley AL, Corson TW, Mehta A, Yeh ES. NF-κB Signaling Is Regulated by Fucosylation in Metastatic Breast Cancer Cells. Biomedicines. 2020; 8(12):600. https://doi.org/10.3390/biomedicines8120600
Chicago/Turabian StyleDoud, Emma H., Trupti Shetty, Melissa Abt, Amber L. Mosley, Timothy W. Corson, Anand Mehta, and Elizabeth S. Yeh. 2020. "NF-κB Signaling Is Regulated by Fucosylation in Metastatic Breast Cancer Cells" Biomedicines 8, no. 12: 600. https://doi.org/10.3390/biomedicines8120600