Dapagliflozin Mitigated Elevated Disomic and Diploid Sperm in a Mouse Model of Diabetes and Recover the Disrupted Ogg1, Parp1, and P53 Gene Expression
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Experimental Diabetes Inductions
2.3. Drug Administration
2.4. Sperm FISH Analysis
2.5. Markers of Redox Imbalance
2.6. Gene Expression Analysis
2.7. Statistics
3. Results
3.1. Dapagliflozin Mitigated Diabetes-Induced Disomy and Diploidy
3.2. Dapagliflozin Abridged Diabetes-Caused Redox Imbalance
3.3. Alterations in Gene Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Forward Primer | Reverse Primer |
---|---|---|
Parp1 | 5′-GGAAAGGGATCTACTTTGCCG-3′ | 5′-TCGGGTCTCCCTGAGATGTG-3′ |
Ogg1 | 5′-GATTGGACAGTGCCGTAA-3′ | 5′-GGAAGTGGGAGTCTACAG-3′ |
P53 | 5′-CACAGCGTGGTGGTACCTTA-3′ | 5′-TCTTCTGTACGGCGGTCTCT-3′ |
GAPDH | 5′-TGCCGCCTGGAGAAACC-3′ | 5′-TGAAGTCGCAGGAGACAACC-3′ |
Non-Diabetic | Non-Diabetic + Dapagliflozin | Diabetes | Diabetes + Dapagliflozin | Non-Diabetic + Colchicine | |
---|---|---|---|---|---|
No. of mice | 6 | 6 | 6 | 6 | 6 |
Total sperm scored | 60,580 | 61,100 | 60,820 | 60,280 | 61,000 |
Y bearing sperm | 30,260 | 30,499 | 30,841 | 29,991 | 30,909 |
X bearing sperm | 30,320 | 30,601 | 29,979 | 30,289 | 30,091 |
Y/X ratio | 1.001 | 1.003 | 0.972 | 1.009 | 0.973 |
Disomy | |||||
X-X-8 | 11 | 10 | 15 | 11 | 14 |
Y-Y-8 | 7 | 6 | 13 | 10 | 13 |
X-Y-8 | 2 | 3 | 9 | 7 | 8 |
X-8-8 | 4 | 6 | 10 | 5 | 10 |
Y-8-8 | 7 | 9 | 13 | 8 | 10 |
Total | 31 | 34 | 60 | 41 | 55 |
% Disomy ± SD | 0.051 ± 0.003 | 0.056 ± 0.003 | 0.10 ± 0.005 ** | 0.068 ± 0.003 b | 0.091 ± 0.006 ## |
Diploidy | |||||
X-Y-8-8 | 1 | 1 | 3 | 1 | 1 |
X-X-8-8 | 1 | 1 | 3 | 2 | 1 |
Y-Y-8-8 | 0 | 1 | 4 | 1 | 1 |
Total | 2 | 3 | 10 | 4 | 3 |
% Diploidy ± SD | 0.0033 ± 0.002 | 0.005 ± 0.002 | 0.0166 ± 0.002 * | 0.006 ± 0.002 a | 0.005 ± 0.002 |
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Albekairi, N.A.; Al-Hamamah, M.A.; Alshamrani, A.A.; Attia, M.S.M.; Nadeem, A.; Ansari, M.A.; Ahmad, S.F.; Bakheet, S.A.; Attia, S.M. Dapagliflozin Mitigated Elevated Disomic and Diploid Sperm in a Mouse Model of Diabetes and Recover the Disrupted Ogg1, Parp1, and P53 Gene Expression. Biomedicines 2023, 11, 2980. https://doi.org/10.3390/biomedicines11112980
Albekairi NA, Al-Hamamah MA, Alshamrani AA, Attia MSM, Nadeem A, Ansari MA, Ahmad SF, Bakheet SA, Attia SM. Dapagliflozin Mitigated Elevated Disomic and Diploid Sperm in a Mouse Model of Diabetes and Recover the Disrupted Ogg1, Parp1, and P53 Gene Expression. Biomedicines. 2023; 11(11):2980. https://doi.org/10.3390/biomedicines11112980
Chicago/Turabian StyleAlbekairi, Norah A., Mohammed A. Al-Hamamah, Ali A. Alshamrani, Mohamed S. M. Attia, Ahmed Nadeem, Mushtaq A. Ansari, Sheikh F. Ahmad, Saleh A. Bakheet, and Sabry M. Attia. 2023. "Dapagliflozin Mitigated Elevated Disomic and Diploid Sperm in a Mouse Model of Diabetes and Recover the Disrupted Ogg1, Parp1, and P53 Gene Expression" Biomedicines 11, no. 11: 2980. https://doi.org/10.3390/biomedicines11112980