Effects of Moringa oleifera Leaf Extract on Diabetes-Induced Alterations in Paraoxonase 1 and Catalase in Rats Analyzed through Progress Kinetic and Blind Docking
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of the Extract
2.2. Ethics Statement
2.3. Diabetic Model and Treatment
2.4. Separation of Serum
2.5. Isolation of Liver Cytosol
2.6. Paraoxonase 1 Activity
2.7. Catalase Activity
2.8. Protein Determination
2.9. Kinetics Data Processing
2.10. Rat Paraoxonase 1 and Catalase Homology Modeling
2.11. Molecular Docking
2.12. Statistical Analysis
3. Results and Discussion
3.1. Effect of M. oleifera Leaves Extract on rPON1 and rCAT Kinetics Parameters
3.2. In-Silico Identification of Potential Interactions between rPON1 and rCAT with Phytochemicals of M. oleifera
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameters | Control (C) | Diabetic (D) | Diabetic + MOE (DM) |
---|---|---|---|
Km | 0.4146 ± 0.04 * μM | 0.325 ± 0.097 *,† μM | 0.0043 ± 0.0026 μM |
Vm | 33.15 ± 4.61 U/(min L) | 17.00 ± 0.99 U/(min L) * | 10.32 ± 3.36 U/(min L) *,† |
Vm/Km | 80.07 ± 2.8 * min−1 | 52.30 ± 9.66 * min−1 | 2400.0 ± 41.74 min−1 |
Parameters | Control (C) | Diabetic (D) | Diabetic + MOE (DM) |
---|---|---|---|
Km | 2.152 ± 0.19 mM | 6.557 ± 0.42 * mM | 60.7 ± 0.03 * mM |
Km | 652.4 ± 8 U/(min L) | 1074 ± 17 U/(min L) * | 6110 ± 22 U/(min L) * |
Vm/Km | 303.16 ± 7.8 min−1 | 1637.94 ± 15.63 min−1 * | 1006.58 ± 10.1 min−1 * |
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Sierra-Campos, E.; Valdez-Solana, M.; Avitia-Domínguez, C.; Campos-Almazán, M.; Flores-Molina, I.; García-Arenas, G.; Téllez-Valencia, A. Effects of Moringa oleifera Leaf Extract on Diabetes-Induced Alterations in Paraoxonase 1 and Catalase in Rats Analyzed through Progress Kinetic and Blind Docking. Antioxidants 2020, 9, 840. https://doi.org/10.3390/antiox9090840
Sierra-Campos E, Valdez-Solana M, Avitia-Domínguez C, Campos-Almazán M, Flores-Molina I, García-Arenas G, Téllez-Valencia A. Effects of Moringa oleifera Leaf Extract on Diabetes-Induced Alterations in Paraoxonase 1 and Catalase in Rats Analyzed through Progress Kinetic and Blind Docking. Antioxidants. 2020; 9(9):840. https://doi.org/10.3390/antiox9090840
Chicago/Turabian StyleSierra-Campos, Erick, Mónica Valdez-Solana, Claudia Avitia-Domínguez, Mara Campos-Almazán, Ismael Flores-Molina, Guadalupe García-Arenas, and Alfredo Téllez-Valencia. 2020. "Effects of Moringa oleifera Leaf Extract on Diabetes-Induced Alterations in Paraoxonase 1 and Catalase in Rats Analyzed through Progress Kinetic and Blind Docking" Antioxidants 9, no. 9: 840. https://doi.org/10.3390/antiox9090840