Effects of Dietary Coated Lysozyme on the Growth Performance, Antioxidant Activity, Immunity and Gut Health of Weaned Piglets
Abstract
:1. Introduction
2. Results
2.1. Growth Performance
2.2. Serum Biochemical Index Analysis
2.3. Immunoglobulin
2.4. Antioxidant Indexes
2.5. Digestive Enzymes
2.6. Morphological Analysis of the Small Intestine
2.7. Quantification of Short-Chain Fatty Acids
2.8. 16S rRNA Sequencing of the Cecal Microflora
3. Discussion
4. Materials and Methods
4.1. Animals, Treatment, and Designation
4.2. Experimental Design
4.3. Sample Collection
4.4. Growth Performance Evaluation
4.5. Determination of Serum Biochemical Index and Immunoglobulin Levels
4.6. Determination of Antioxidant Indexes
4.7. Digestive Enzymes
4.8. Morphological Analysis of the Small Intestine
4.9. Determination of Short-Chain Fatty Acid Levels
4.10. 16S rRNA Sequencing of the Cecal Microflora
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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CN | CL-L | CL-M | CL-H | UL | Abs | SEM | p Value | |
---|---|---|---|---|---|---|---|---|
(d 0) Initial weight (kg) | 8.66 ± 0.95 | 8.71 ± 0.31 | 9.18 ± 0.24 | 8.91 ± 0.25 | 8.62 ± 0.34 | 9.08 ± 0.49 | 85.07 | 0.299 |
(d 14) Middle weight (kg) | 12.74 ± 0.80 c | 13.11 ± 0.25 c | 14.26 ± 0.51 abc | 14.60 ± 0.31 ab | 13.78 ± 1.12 bc | 16.06 ± 0.19 a | 222.54 | 0.007 |
(d 28) Final weight (kg) | 16.88 ± 1.30 b | 1.81 ± 0.19 b | 19.07 ± 0.77 b | 20.84 ± 0.67 a | 18.47 ± 1.60 b | 21.98 ± 0.47 a | 373.22 | 0.000 |
ADG (g) | ||||||||
Days 0 to 14 | 339.13 ± 49.85 bc | 313.87 ± 14.54 c | 362.67 ± 35.29 bc | 406.24 ± 4.50 ab | 368.33 ± 61.36 bc | 451.23 ± 21.60 a | 13.08 | 0.009 |
Days 14 to 28 | 295.71 ± 40.92 c | 358.77 ± 22.72 abc | 343.62 ± 18.86 bc | 445.74 ± 68.57 a | 334.91 ± 81.18 bc | 422.80 ± 41.55 ab | 16.09 | 0.029 |
Days 0 to 28 | 317.42 ± 24.74 b | 336.32 ± 8.75 b | 353.14 ± 25.86 b | 425.99 ± 32.48 a | 351.62 ± 55.86 b | 437.01 ± 26.31 a | 12.64 | 0.003 |
ADFI (g) | 672.57 ± 4.92 a | 613.50 ± 11.54 b | 605.60 ± 10.66 b | 649.26 ± 22.38 a | 544.97 ± 12.35 c | 673.90 ± 21.50 a | 11.31 | 0.000 |
F/G | 2.13 ± 0.15 a | 1.82 ± 0.05 b | 1.72 ± 0.11 b | 1.53 ± 0.06 c | 1.58 ± 0.28 b | 1.54 ± 0.05 c | 0.06 | 0.002 |
CN | CL-L | CL-M | CL-H | UL | Abs | SEM | p Value | |
---|---|---|---|---|---|---|---|---|
CAT (U/mL) | 169.01 ± 56.16 b | 242.58 ± 51.59 a | 263.70 ± 50.18 a | 281.09 ± 44.31 a | 280.33 ± 54.93 a | 291.10 ± 50.60 a | 10.77 | 0.003 |
GSH-Px (U/L) | 80.03 ± 11.93 d | 105.68 ± 23.53 c | 103.72 ± 13.89 c | 135.71 ± 18.13 ab | 145.50 ± 14.28 a | 118.44 ± 20.24 bc | 4.62 | 0.000 |
MDA (nmol/mL) | 5.97 ± 1.20 a | 5.61 ± 1.12 ab | 4.20 ± 1.04 bc | 4.39 ± 1.38 bc | 3.25 ± 1.37 c | 5.39 ± 1.04 ab | 0.25 | 0.005 |
SOD (U/mL) | 195.03 ± 45.01 d | 258.78 ± 39.32 ab | 300.52 ± 51.47 abc | 329.88 ± 52.14 ab | 343.96 ± 53.61 a | 270.14 ± 63.65 bc | 11.79 | 0.000 |
T-AOC (μmol/mL) | 1.54 ± 0.31 c | 2.05 ± 0.23 b | 2.19 ± 0.35 ab | 2.49 ± 0.54 a | 2.58 ± 0.25 a | 2.07 ± 0.35 b | 0.08 | 0.000 |
CN | CL-L | CL-M | CL-H | UL | Abs | SEM | p Value | |
---|---|---|---|---|---|---|---|---|
acetic acid | 22.54 ± 8.77 a | 14.64 ± 2.30 c | 13.42 ± 2.90 c | 15.74 ± 2.41 ab | 14.47 ± 2.75 c | 17.53 ± 4.72 ab | 1.04 | 0.113 |
propionic acid | 8.71 ± 2.85 a | 6.34 ± 0.66 ab | 5.40 ± 1.34 b | 6.39 ± 0.76 ab | 5.87 ± 0.58 b | 7.80 ± 2.42 ab | 0.39 | 0.100 |
isobutyric acid | 0.66 ± 0.15 a | 0.56 ± 0.09 a | 0.44 ± 0.12 a | 0.61 ± 0.14 a | 0.60 ± 0.16 a | 0.63 ± 0.29 a | 0.03 | 0.521 |
butyric acid | 4.16 ± 1.05 a | 2.60 ± 0.61 bc | 1.92 ± 0.74 c | 2.69 ± 0.43 bc | 2.13 ± 1.07 bc | 3.37 ± 0.62 ab | 0.21 | 0.008 |
isovaleric acid | 1.11 ± 0.25 a | 0.90 ± 0.16 a | 0.72 ± 0.18 a | 1.05 ± 0.20 a | 1.05 ± 0.35 a | 1.15 ± 0.41 a | 0.06 | 0.284 |
valeric acid | 0.80 ± 0.16 ab | 0.68 ± 0.08 ab | 0.59 ± 0.10 b | 0.85 ± 0.09 a | 0.71 ± 0.14 ab | 0.87 ± 0.21 a | 0.03 | 0.075 |
Diet Composition | Percentage (%) | Nutrient Levels | Content |
---|---|---|---|
Corn | 62.12 | Total (MJ/kg) | 14.55 |
Bean meal | 24.88 | Crude protein | 17.4 |
Wheat bran | 3 | Total phosphorus | 0.51 |
Fatty powder | 2.0 | Lysine | 1.158 |
Limestone | 1.2 | Methionine+cysteine | 0.612 |
Calcium hydrogen phosphate | 0.8 | Threonine | 0.679 |
Soybean oil | 0.07 | ||
Fish meal | 1 | ||
NaCl | 0.3 | ||
Choline chloride | 0.1 | ||
Potassium magnesium sulfate | 0.2 | ||
Premix * | 1 |
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Xu, X.; Huang, P.; Cui, X.; Li, X.; Sun, J.; Ji, Q.; Wei, Q.; Huang, Y.; Li, Z.; Bao, G.; et al. Effects of Dietary Coated Lysozyme on the Growth Performance, Antioxidant Activity, Immunity and Gut Health of Weaned Piglets. Antibiotics 2022, 11, 1470. https://doi.org/10.3390/antibiotics11111470
Xu X, Huang P, Cui X, Li X, Sun J, Ji Q, Wei Q, Huang Y, Li Z, Bao G, et al. Effects of Dietary Coated Lysozyme on the Growth Performance, Antioxidant Activity, Immunity and Gut Health of Weaned Piglets. Antibiotics. 2022; 11(11):1470. https://doi.org/10.3390/antibiotics11111470
Chicago/Turabian StyleXu, Xiangfei, Pan Huang, Xuemei Cui, Xuefeng Li, Jiaying Sun, Quanan Ji, Qiang Wei, Yee Huang, Zhefeng Li, Guolian Bao, and et al. 2022. "Effects of Dietary Coated Lysozyme on the Growth Performance, Antioxidant Activity, Immunity and Gut Health of Weaned Piglets" Antibiotics 11, no. 11: 1470. https://doi.org/10.3390/antibiotics11111470