Phytochemical Screening and Antioxidant Activity of Trichosanthes cucumerina, Momordica charantia var muricata and Luffa acutangula †
by
,
Vikas Gautam
1,*
,
Anandika Suryavanshi
1, 
Naushad Ahmad Shah
2,
Kumar Gaurav Bajpai
2,*,
Syed Shabihe Raza Baqri
2,
Taiyab Shamshad Naqvi
2 and
Anand Murari Saxena
1 1
Department of Zoology, University of Lucknow, Lucknow 226007, India
2
Department of Zoology, Shia P.G. College, Sitapur Road, Lucknow 226020, India
*
Authors to whom correspondence should be addressed.
†
Presented at the 2nd International Electronic Conference on Biomolecules: Biomacromolecules and the Modern World Challenges, 1–15 November 2022; Available online: https://iecbm2022.sciforum.net/ .
Biol. Life Sci. Forum 2022, 20(1), 32; https://doi.org/10.3390/IECBM2022-13694
Published: 17 November 2022
(This article belongs to the Proceedings of The 2nd International Electronic Conference on Biomolecules: Biomacromolecules and the Modern World Challenges)
Background—TC, LA and MCM plants(family: Cucurbitaceae) are widely used in traditional medicine and are important sources of vegetables in the world. Many of these are known to have important medicinal properties and have been recommended in traditional medicine for various ailments [1]. Objectives—This study aimed to evaluate the phytochemical constituents of test plants (TA, LA and MCM) of 95% ethanolic whole-plant extracts, as well as to study the antioxidant activity using DPPH assay.Materials and Methods—The plants were powdered and mechanically extracted using the soxhlet apparatus [2], which was followed by phytochemical screening of the extracts. Various classes of phytochemicals (viz., alkaloids, phenols, steroids, glycosides and saponins) were screened using standard methods [3,4,5]. The antioxidant activity was determined using DPPH assay [6]. Results—The phytochemical screening revealed the presence of glycosides in TC, saponins in MCM and LA and alkaloids in TC and MC plants. However, phenols and steroids were found in all three plant species (TC, LA and MCM). The DPPH assay to test the antioxidant activity involved the measurement of IC50 and percentage inhibition with respect to AA. Results showed that the DPPH free radicals were scavenged by all the extracts in a concentration-dependent manner. Conclusions—These dietary cucurbits showed appreciable antioxidant activity and are good sources of natural antioxidants. Future pharmaceutical uses can be deduced from these findings.
Supplementary Materials
The presentation material of this work is available online at https://www.mdpi.com/article/10.3390/IECBM2022-13694/s1.
Author Contributions
Conceptualization, V.G.; methodology, V.G. and A.S.; software, V.G.; validation, V.G., A.S. and N.A.S.; formal analysis, S.S.R.B.; investigation, V.G.; resources, A.M.S.; data curation, N.A.S. and K.G.B.; writing—original draft preparation, V.G.; writing—review and editing, S.S.R.B. and T.S.N.; visualization, V.G. and A.S.; supervision, A.M.S.; project administration, V.G. and K.G.B. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
The data presented in this study are available in Supplementary Material.
Conflicts of Interest
The authors declare no conflict of interest.
Abbreviations
TC: Trichosanthes cucumerina, LA: Luffa acutangula, MCM: Momordica charantia-muricata, DPPH: 2,2-Diphenyl-1-picrylhydrazyl, AA: Ascorbic Acid.
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MDPI and ACS Style
Gautam, V.; Suryavanshi, A.; Shah, N.A.; Bajpai, K.G.; Baqri, S.S.R.; Naqvi, T.S.; Saxena, A.M. Phytochemical Screening and Antioxidant Activity of Trichosanthes cucumerina, Momordica charantia var muricata and Luffa acutangula. Biol. Life Sci. Forum 2022, 20, 32. https://doi.org/10.3390/IECBM2022-13694
AMA Style
Gautam V, Suryavanshi A, Shah NA, Bajpai KG, Baqri SSR, Naqvi TS, Saxena AM. Phytochemical Screening and Antioxidant Activity of Trichosanthes cucumerina, Momordica charantia var muricata and Luffa acutangula. Biology and Life Sciences Forum. 2022; 20(1):32. https://doi.org/10.3390/IECBM2022-13694
Chicago/Turabian StyleGautam, Vikas, Anandika Suryavanshi, Naushad Ahmad Shah, Kumar Gaurav Bajpai, Syed Shabihe Raza Baqri, Taiyab Shamshad Naqvi, and Anand Murari Saxena. 2022. "Phytochemical Screening and Antioxidant Activity of Trichosanthes cucumerina, Momordica charantia var muricata and Luffa acutangula" Biology and Life Sciences Forum 20, no. 1: 32. https://doi.org/10.3390/IECBM2022-13694
